腺苷酸环化酶抑制剂和激动剂在脂多糖诱导的急性肺损伤中的作用

发布时间：2018-05-10 16:39

本文选题：急性肺损伤 + 内毒素　；参考：《中国药理学通报》2017年10期

【摘要】：目的探讨腺苷酸环化酶抑制剂(SQ22536)和激动剂(Forskolin)在脂多糖(LPS)诱导的急性肺损伤(acute lung injury,ALI)中的作用。方法 ICR小鼠随机分为生理盐水组(N组)、模型组(L组)、地塞米松组(D组)、SQ22536组(SQ组)和Forskolin组(F组),气道内滴入LPS制备ALI模型。6 h后观察肺组织病理改变,测定肺泡灌洗液(BALF)中白细胞、中性粒细胞和白蛋白含量,检测肺组织中髓过氧化物酶(MPO)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL~(-1)β)、白介素-6(IL-6)和c AMP含量。结果 Forskolin可明显改善肺组织病理变化,降低BALF中白细胞、中性粒细胞和蛋白含量,MPO活性和TNF-α含量明显降低,c AMP含量升高;SQ22536与M组相比差异均无显著性。结论 Forskolin对ALI的保护机制可能与升高c AMP水平、抑制中性粒细胞黏附和趋化及降低TNF-α等相关炎症因子含量有关。
[Abstract]:Objective to investigate the role of adenylate cyclase inhibitor SQ22536) and agonist Forskolin in acute lung injury induced by lipopolysaccharide (LPS). Methods ICR mice were randomly divided into normal saline group (n group), model group (L group), dexamethasone group (group D) and Forskolin group (group F). The pathological changes of lung tissue were observed after LPS was dripped into the airway for 6 h. The contents of leukocytes, neutrophils and albumin in alveolar lavage fluid (BALF), myeloperoxidase (MPO), tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 (IL-1) 尾, interleukin-6 (IL-6) and c AMP were measured. Results Forskolin could significantly improve the pathological changes of lung tissue and decrease the contents of leukocyte, neutrophil and protein in BALF. The activity of MPO and the content of TNF- 伪 in BALF were significantly decreased. There was no significant difference between SQ22536 and M group. Conclusion the protective mechanism of Forskolin on ALI may be related to increasing the level of c AMP, inhibiting the adhesion and chemotaxis of neutrophils and decreasing the content of inflammatory cytokines such as TNF- 伪.
【作者单位】：浙江中医药大学附属第二医院药剂科;浙江大学医学院;
【基金】：国家自然科学基金资助项目(No 81202980)
【分类号】：R563.8

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