老年大鼠创伤性脑损伤后血管再生调节因子表达与肠黏膜、肠屏障功能的变化

发布时间：2018-05-11 07:22

本文选题：老年大鼠 + 创伤性脑损伤　；参考：《华中科技大学学报(医学版)》2016年04期

【摘要】：目的观察老年大鼠创伤性脑损伤后血管再生调节因子表达与肠黏膜、肠屏障功能的变化。方法选择体重为500~600g,17~20个月月龄的健康老年雄性SD大鼠,制备老年大鼠创伤性脑损伤模型,按损伤时间分为伤后3、12、24、72h和5d,假手术组为对照组,每组10只。记录和比较各组大鼠行为学、脑组织含水量、肠黏膜显微特征、肠屏障功能和脑组织中VEGF和CD34蛋白表达。结果与对照组比较,创伤性颅脑损伤3h~5d,随着损伤时间的延长,大鼠的行为学评分、脑组织含水量、脑组织中VEGF和CD34蛋白表达均明显增高(均P0.05),肠绒毛高度、绒毛中段直径、隐窝深度和绒毛表面积均明显降低(均P0.05);肠黏膜绒毛的破坏度明显,绒毛间质疏松,肠绒毛排列不规则甚至断裂,绒毛上皮坏死脱落,变矮,间距增宽,固有层出现不同程度水肿,上述异常在伤后24~72h达到高峰(均P0.05)。结论密切关注老年大鼠创伤性脑损伤后24~72h的病情转归对降低病残和致死率应有积极意义,增高VEGF和CD34表达,促进血管再生可能是治疗创伤性脑损伤后继发性脑损害的新思路。
[Abstract]:Objective to observe the expression of angiogenesis regulatory factors and the changes of intestinal mucosal and intestinal barrier function after traumatic brain injury in aged rats. Methods healthy male Sprague-Dawley (SD) rats aged from 17 to 20 months, weighing 500 ~ 600g / kg, were selected to establish traumatic brain injury model in old rats. The rats were divided into two groups according to the time of injury: the rats were divided into two groups: the control group (n = 10) and the sham-operated group (n = 10) for 72 hours and 5 days after injury. The behavior, water content of brain tissue, intestinal mucosal microscopic characteristics, intestinal barrier function and the expression of VEGF and CD34 protein in brain tissue were recorded and compared in each group. Results compared with the control group, the behavioral score, brain water content and the expression of VEGF and CD34 protein in brain tissue were significantly higher than those in the control group (P0.05, villi height, midvilli diameter) with the prolongation of the injury time. The depth of crypt and the surface area of villi decreased significantly (P0.05%), the degree of destruction of intestinal villi was obvious, the interstitial villi were loose, the villi arranged irregularly or even broken, the villi necrotic and shedding, the villi became shorter, and the spacing widened. Edema appeared in lamina propria to different degrees, and the above abnormality reached its peak at 2472 hours after injury (all P 0.05). Conclusion paying close attention to the outcome of traumatic brain injury 2472 h after traumatic brain injury in aged rats should have a positive significance in reducing disability and mortality. Increasing the expression of VEGF and CD34 and promoting vascular regeneration may be a new way to treat secondary brain damage after traumatic brain injury.
【作者单位】：江苏大学附属金坛医院神经外科;中国人民解放军101医院;
【分类号】：R651.15

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