硫化氢对组织因子诱导的弥散性血管内凝血家兔体外凝血活性的影响

发布时间：2018-05-11 13:57

本文选题：硫化氢 + 弥散性血管内凝血　；参考：《中国老年学杂志》2016年02期

【摘要】：目的探讨硫化氢对组织因子诱导的弥散性血管内凝血(DIC)家兔凝血活性的影响。方法健康青紫蓝家兔通过静脉注射组织因子复制DIC模型。采用全自动凝血分析仪检测活化部分凝血活酶时间(APTT)和凝血酶原时间(PT);凝聚法检测血浆纤维蛋白(原)降解产物(FDP)。结果注射组织因子至5 min时,APTT和PT较注射前缩短(P0.05),15、45 min时,APTT和PT较注射前延长(P0.05),注射组织因子后的血样品,FDP检测均出现乳胶颗粒凝集;经硫化氢处理,注射组织因子前血浆PT无明显变化,中、高剂量组APTT延长(P0.05),FDP检测未出现乳胶颗粒凝集;注射组织因子5 min时,中、高剂量组PT较对照组延长(P0.05),高剂量组APTT较对照组延长(P0.05);15、45 min时,PT仅在高剂量时较对照组延长(P0.05),APTT在中、高剂量时较对照组均延长(P0.05);FDP检测,5和15 min时中、高剂量组的乳胶颗粒凝集较对照组明显减弱,45 min时,各组的乳胶颗粒凝集均不明显。结论硫化氢对实验性DIC动物体外凝血有一定的抑制作用。
[Abstract]:Objective to investigate the effect of hydrogen sulfide on the coagulation activity of DICs induced by tissue factor. Methods DIC model was induced by intravenous injection of tissue factor in healthy turquoise rabbits. The activated partial thromboplastin time (APTT) and prothrombin time (PTT) were detected by automatic coagulation analyzer and the plasma fibrin (proto) degradation product was detected by coagulation method. Results APTT and PT at 5 min after injection were shorter than those at 1545 min before injection, and the blood samples after injection of tissue factor showed latex agglutination, and were treated with hydrogen sulfide. There was no significant change in plasma PT before injection of tissue factor, and no agglutination of latex particles was observed in high dose group (P 0.05), but at 5 min after injection of tissue factor, no agglutination of latex particles was observed in high dose group. PT in the high dose group was longer than that in the control group (P 0.05), and the APTT in the high dose group was longer than that in the control group at 1545 min. The PT in the high dose group was only longer than that in the control group at the middle and middle levels, and in the high dose group compared with the control group, it was also prolonged at 5 and 15 min. The agglutination of latex particles in the high dose group was less than that in the control group for 45 min. Conclusion hydrogen sulfide can inhibit the coagulation of experimental DIC animals in vitro.
【作者单位】：皖南医学院临床医学院;皖南医学院基础医学院;
【基金】：国家自然科学基金项目(81172790) 安徽省大学生创新创业训练项目(AH201310368098)
【分类号】：R554.8

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