极化液对脓毒症休克兔肾脏保护作用研究

发布时间：2018-05-19 23:09

本文选题：脓毒症休克 + 肾损伤　；参考：《川北医学院》2017年硕士论文

【摘要】：目的:通过耳缘静脉注射脂多糖(lipopolysaccharide,LPS)建立新西兰兔脓毒症休克模型,以静脉持续泵注极化液干预,研究极化液对脓毒症休克兔肾脏损伤的影响。方法:30只新西兰大白兔随机分成三组。脂多糖组(LPS组,n=10)和极化液治疗组(GIK组,n=10):经耳缘静脉注射脂多糖0.5mg/kg,正常对照组(N组,n=10):耳缘静脉注射等剂量生理盐水。造模成功后,GIK组经耳缘静脉持续泵注极化液(5ml/kg/h),N组和LPS组以相同的速度泵注复方氯化钠溶液。连续监测三组兔的生命体征;分别在造模前、造模成功后以及治疗3、6小时后抽取血液标本检测血糖、乳酸、血钾、Cys-C水平;治疗6小时后处死动物,检测肾脏TNF-ɑ、MDA、Caspase-3水平,HE染色观察病理变化情况,电镜观察线粒体损伤情况。结果:1.生命体征改变:与N组相比,LPS组兔表现为呼吸加快、平均动脉压降低、体温升高等(P0.05),GIK组兔以上表现相较LPS组有所减轻。2.血气分析的变化:与N组相比,LPS组血糖升高、乳酸明显升高、PCO2降低、血钾升高(P0.05),GIK组与LPS组相比乳酸、血钾的水平降低(P0.05)。3.肾脏损伤指标的变化:与N组相比,LPS组与GIK组TNF-ɑ、Cys-C、MDA水平明显升高(P0.05),LPS组较GIK组更高(P0.05);HE染色显示GIK干预组肾脏病理损伤轻于LPS组,N组损伤轻于GIK组。4.细胞凋亡指标结果:与LPS组相比,GIK组Caspase-3的表达下调(P0.05)。与N组相比,GIK组Caspase-3的表达上调(P0.05)。5.电镜结果:与N组相比,LPS组肾小管上皮细胞内线粒体肿胀明显,而GIK组线粒体结构清晰可见,无膜断裂,肿胀较LPS组轻。结论:GIK能通过减轻线粒体损伤、减少肾组织细胞凋亡,对LPS诱导的脓毒症休克性兔肾损伤具有保护作用。
[Abstract]:Aim: to establish a septic shock model in New Zealand rabbits by intravenous injection of lipopolysaccharide and lipopolysaccharide (LPSs) at the ear edge, and to study the effect of pollination solution on renal injury in rabbits with septic shock. Methods 30 New Zealand white rabbits were randomly divided into three groups. Lipopolysaccharide (LPS) group (LPS-10) and Polarizate treatment group (GIK group): LPS-0.5 mg / kg was injected via ear margin, and normal control group (n group) was injected intravenously with normal saline. After the establishment of the model, the GIK group was injected with 5 ml / kg 路kg ~ (-1) 路h ~ (-1) of poling solution through the auricular vein at the same speed as the LPS group. The vital signs of the three groups of rabbits were continuously monitored, blood samples were collected before, after successful modeling and 3 hours after treatment for blood glucose, lactic acid, blood potassium and Cys-C levels, and the animals were killed after 6 hours of treatment. The level of Caspase-3 in renal TNF- MDA-Caspase-3 was detected by HE staining and mitochondrial damage was observed by electron microscope. The result is 1: 1. The changes of vital signs: compared with group N, lipopolysaccharide group showed faster respiration, lower mean arterial pressure and elevated body temperature, etc. The above manifestations of P0.05GIK group were lighter than those of LPS group. 2. The changes of blood gas analysis: compared with the N group, the blood glucose increased, lactic acid increased significantly and PCO _ 2 decreased in the LPS group, and the level of blood potassium in the GIK group was significantly lower than that in the LPS group, compared with that in the LPS group. The changes of renal injury index: compared with N group, the level of TNF-Cys-Cn MDA in LPS group and GIK group was significantly higher than that in GIK group. The pathological damage of kidney in GIK intervention group was lighter than that in LPS group and GIK group. 4. Results of apoptosis: compared with LPS group, the expression of Caspase-3 in GIK group was down-regulated (P 0.05). Compared with N group, the expression of Caspase-3 was up-regulated in GIK group. The results of electron microscope showed that the mitochondria swelling of tubular epithelial cells in lipopolysaccharide group was more obvious than that in N group, while the mitochondrial structure in GIK group was clearly visible, and there was no rupture of membrane, and the swelling was lighter than that in LPS group. ConclusionGIK can attenuate mitochondria injury and decrease apoptosis of renal tissue, and it has protective effect on septic shock injury induced by LPS in rabbits.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R459.7

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