急性ST段抬高型心肌梗死患者的血清代谢组学研究

发布时间：2018-05-20 00:02

本文选题：急性ST段抬高型心肌梗死 + 代谢组学　；参考：《重庆医科大学学报》2016年09期

【摘要】：目的:分析急性ST段抬高型心肌梗死(acute ST-segment elevation myocardial infarction,ASTEMI)患者的血清,找出特异性的代谢产物。方法:运用超高效液相色谱与四级杆飞行时间串联质谱仪联用技术(ultraperformance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry,UPLC-Q-TOF-MS/MS)和多维统计分析结合单维统计分析的方法,比较ASTEMI患者组(n=15)和健康对照组(n=10)的血清。同时采用化学发光法检测ASTEMI患者组(n=15)和健康对照组(n=10)血清中的硫酸脱氢表雄酮(dehydroepiandrosterone sulfate,DHEAS)含量,进一步证实ASTEMI患者的部分代谢组学研究结果。结果:经UPLC-Q-TOF-MS/MS分析后发现ASTEMI患者组和健康对照组具有不同的代谢特征。在多维统计分析中,主成分分析(principal component analysis,PCA)和偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)结果显示ASTEMI患者组和健康对照组分布在不同区域。结合多维统计分析和单维统计分析方法,发现ASTEMI患者组血清中至少有9个代谢产物明显不同于健康对照组。经过代谢组学研究发现的部分差异(ASTEMI患者组血清中的DHEAS含量明显低于健康对照组),进一步采用化学发光法检测证实(P=0.000)。结论:通过代谢组学分析发现ASTEMI患者血清代谢水平明显不同于健康对照者。在ASTEMI患者血清中至少有9个代谢产物与该疾病相关。这些发现可能提供关于ASTEMI患者代谢改变的新见解。
[Abstract]:Aim: to analyze the serum of patients with acute St segment elevation myocardial infarction (St segment elevation myocardial infarction) and to find out the specific metabolites. Methods: ultrahigh performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectroscopy (UPLC-Q-TOF-MS / MS) and multidimensional statistical analysis combined with one-dimensional statistical analysis were used to compare the serum levels of ASTEMI patients (n = 15) and healthy controls (n = 10) by ultra-high performance liquid chromatography (HPLC) and time-of-flight mass spectrometer (TMS). At the same time, the contents of dehydroepiandrosterone sulfate sulfated DHEASA in the serum of patients with ASTEMI (n = 15) and healthy control group (n = 10) were determined by chemiluminescence assay. The results of some metabolic studies in patients with ASTEMI were further confirmed. Results: UPLC-Q-TOF-MS/MS analysis showed that ASTEMI patients and healthy controls had different metabolic characteristics. In multidimensional statistical analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DAA) showed that ASTEMI patients and healthy controls were distributed in different regions. Combined with multidimensional statistical analysis and one-dimensional statistical analysis, it was found that at least 9 metabolites in serum of ASTEMI patients were significantly different from those of healthy controls. The serum DHEAS content in ASTEMI group was significantly lower than that in healthy control group, and was further confirmed by chemiluminescence assay. Conclusion: the serum metabolic level of ASTEMI patients was significantly different from that of healthy controls. At least 9 metabolites in the serum of ASTEMI patients are associated with the disease. These findings may provide new insights into metabolic changes in patients with ASTEMI.
【作者单位】：重庆医科大学附属第一医院心血管内科;重庆医科大学生命科学院;美国俄亥俄州立大学心肺研究所;
【基金】：国家重点基础研究发展计划资助项目(编号:2014CB542400) 国家自然科学基金资助项目(编号:81170112、81270210) 国家临床重点专科建设经费资助项目(编号:财社(2011)170号) 重庆市科委资助项目(编号:cstc2012jj A10143)
【分类号】：R542.22

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