立体定向脑内移植神经干细胞改善颅脑损伤大鼠的神经运动功能

发布时间：2018-05-20 14:16

  本文选题：神经干细胞 + 颅脑损伤　； 参考：《中国组织工程研究》2016年10期

【摘要】：背景:细胞替代治疗是重建受损神经系统组织结构,恢复神经系统功能的一种有效策略,具有极为广阔的应用前景。目的:探讨立体定向脑内移植神经干细胞对颅脑损伤大鼠神经运动功能的影响。方法:将20只雄性SD大鼠随机分为研究组和对照组,每组10只,应用改良的自由落体方法制备脑损伤模型,伤后1 d研究组大鼠脑实质内移植胚胎神经干细胞,对照组大鼠注射等量不含干细胞的培养液。在脑损伤前1 d、伤后1 d、1周、2周运用神经学缺损评分法评价大鼠运动神经功能。移植后2周,取脑组织分别行苏木精-伊红染色、抗BrdU、胶质纤维酸性蛋白、β微管蛋白Ⅲ和酪氨酸羟化酶免疫组化染色。结果与结论:伤后1,2周研究组大鼠的神经学缺损评分均显著低于对照组(P0.05)。研究组大鼠受损脑组织中有较多的Brd U阳性神经干细胞,一部分细胞呈胶质纤维酸性蛋白、β微管蛋白Ⅲ和酪氨酸羟化酶阳性表达,对照组大鼠受损脑组织中未见有Brd U阳性细胞。实验结果表明,立体定向脑内移植神经干细胞可以在颅脑损伤灶中增殖分化,进而显著改善大鼠的神经运动功能。
[Abstract]:Background: cell replacement therapy is an effective strategy to reconstruct the injured nervous system and restore the function of the nervous system. Aim: to investigate the effect of stereotactic neural stem cells transplantation on neural motor function in rats with craniocerebral injury. Methods: twenty male Sprague-Dawley rats were randomly divided into study group and control group with 10 rats in each group. The model of brain injury was established by modified free-fall method. Embryonic neural stem cells were transplanted into the brain of the study group 1 day after injury. Rats in the control group were injected with the same amount of culture medium without stem cells. The motor nerve function of rats was evaluated 1 day before and 1 week and 2 weeks after brain injury. Two weeks after transplantation, the brain tissues were stained with hematoxylin-eosin, anti-BrdU, glial fibrillary acidic protein, 尾 -tubulin 鈪，

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