塞来昔布对颅脑创伤后大鼠的凋亡蛋白Bcl-2和Bax表达影响

发布时间：2018-05-24 10:58

本文选题：塞来昔布 + 颅脑损伤　；参考：《中国临床药理学杂志》2017年09期

【摘要】：目的研究选择性环氧合酶-2抑制剂——塞来昔布对大鼠颅脑创伤后凋亡蛋白Bcl-2和Bax表达的影响。方法按照体重将SD大鼠随机分为4组:实验组、模型组、假手术组和对照组,实验组和模型组构建大鼠闭合性颅脑创伤模型。在颅脑损伤模型构建后,实验组立即经腹腔注入塞来昔布250mg·kg-1;模型组、假手术组和对照组给于同等量的0.9%Na Cl腹腔注射。用免疫印迹法和免疫组织化学染色法检测凋亡蛋白Bcl-2和Bax的表达。结果实验组、模型组、假手术组和对照组的各组Bcl-2蛋白表达量分别为68.75±0.62,45.40±0.36,81.08±0.65,78.12±0.05,与假手术组和对照组比较,模型组Bcl-2蛋白表达量明显降低,差异有统计学意义(P0.05);与模型组比较,实验组Bcl-2蛋白表达量明显增加,差异有统计学意义(P0.05)。实验组、模型组、假手术组和对照组的各组Bax蛋白表达量分别为68.28±0.35,92.48±0.16,65.06±0.63,63.15±0.10,与假手术组和对照组比较,模型组Bax蛋白表达量明显增加,差异有统计学意义(P0.05);与模型组相比较,实验组Bax蛋白表达量明显降低,差异有统计学意义(P0.05)。结论塞来昔布可通过增加脑组织Bcl-2的表达、降低Bax的表达和抑制脑损伤后神经细胞的凋亡,对大鼠颅脑创伤具有保护作用。
[Abstract]:Objective to study the effect of selective cyclooxygenase -2 inhibitor - celecoxib on the expression of apoptotic protein Bcl-2 and Bax in rats after craniocerebral trauma. Methods SD rats were randomly divided into 4 groups according to body weight: experimental group, model group, sham operation group and control group, experimental group and model group constructed rat model of closed craniocerebral trauma. The model of brain injury in the experimental group and model group. After the construction, the experimental group was immediately injected with celecoxib 250mg kg-1; the model group, the sham operation group and the control group were given the same amount of 0.9%Na Cl intraperitoneally. The expression of Bcl-2 and Bax of apoptotic protein was detected by immunoblotting and immunohistochemistry. Results of the experimental group, model group, sham operation group and control group Bcl-2 protein table The amount of the expression was 68.75 + 0.62,45.40 + 0.36,81.08 + 0.65,78.12 + 0.05 respectively. Compared with the sham operation group and the control group, the expression of Bcl-2 protein in the model group decreased significantly (P0.05). Compared with the model group, the expression of Bcl-2 protein in the experimental group was significantly increased (P0.05). The experimental group, the model group, the sham operation group and the experimental group were statistically significant (P0.05). The expression of Bax protein in the control group was 68.28 + 0.35,92.48 + 0.16,65.06 + 0.63,63.15 + 0.10 respectively. Compared with the sham operation group and the control group, the expression of Bax protein in the model group was significantly increased, the difference was statistically significant (P0.05). Compared with the model group, the expression of Bax protein in the experimental group was significantly reduced, the difference was statistically significant (P0.05). Celecoxib can increase the expression of Bcl-2 in brain tissue, reduce the expression of Bax and inhibit the apoptosis of nerve cells after brain injury. It has protective effect on brain trauma in rats.
【作者单位】：河北医科大学附属邢台人民医院神经外科;邢台医学高等专科学校药学系;华北理工大学基础医学院;
【基金】：河北省自然科学基金资助项目(C2004000689) 河北省博士基金资助项目(05547008D-4) 河北省科学技术与社会发展计划基金资助项目(04276135)
【分类号】：R651.15

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