C-sis基因对大鼠暴发性肝衰竭的抑制作用

发布时间：2018-05-29 01:37

本文选题：C-sis + 大鼠　；参考：《广东医学》2017年12期

【摘要】：目的探讨C-sis基因对暴发性肝衰竭(FHF)的影响。方法将重组质粒pc DNA3.1/C-sis注射入鼠尾静脉导入大鼠肝脏,48 h后用内毒素(LPS)联合D-半乳糖胺(D-GalN)处理大鼠建立FHF模型;实验分组:正常对照组,FHF+林格氏液注射组,FHF+空载质粒组,FHF+C-sis质粒组;利用荧光定量PCR和Western blot检测C-sis的表达;计算实验大鼠的死亡率;利用TUNEL来检测细胞凋亡;检测实验大鼠血清中丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)的含量。结果荧光定量PCR和Western blot结果显示,与正常对照组和FHF+空载质粒组比较,FHF+C-sis质粒组的C-sis mRNA和蛋白表达量明显增加(P均0.01)。在24 h观察期内,正常对照组大鼠均存活,在FHF+林格氏液注射组和FHF+空载质粒组24 h内分别有70.00%和80.00%的大鼠死亡;而FHF+C-sis质粒组在24 h的观察期内死亡率仅20.00%。TUNEL检测结果显示,与空白对照组相比,FHF+林格氏液注射组肝脏组织中细胞凋亡增多(P0.01);与FHF+空载质粒组相比,FHF+Csis质粒组肝脏组织中细胞凋亡减少(P0.01)。与正常对照组比较,FHF+林格氏液注射组的ALT(P0.01)和AST(P0.05)含量升高;与FHF+空载质粒组比较,FHF+C-sis质粒组的ALT(P0.01)和AST(P0.05)含量降低。结论 C-sis基因能抑制LPS联合D-GalN诱导的大鼠FHF。
[Abstract]:Objective to investigate the effect of C-sis gene on fulminant liver failure. Methods the recombinant plasmid PC DNA3.1/C-sis was injected into the rat caudal vein for 48 h. The rat model of FHF was established by the combination of lipopolysaccharide (LPS) and D-galactosamine D-GalN (D-GalN). The experiment was divided into three groups: the normal control group was injected with FHF-Ringerian solution, the FHF-free plasmid group was injected with FHF-free plasmid group, the expression of C-sis was detected by fluorescence quantitative PCR and Western blot, the death rate of experimental rats was calculated, and apoptosis was detected by TUNEL. Serum alanine aminotransferase (alt) and aspartate aminotransferase (AST) were measured. Results the results of fluorescence quantitative PCR and Western blot showed that the expression of C-sis mRNA and protein in FHF C-sis plasmid group was significantly higher than that in normal control group and FHF no-load plasmid group. During the 24 h observation period, all the normal control rats survived, 70.00% and 80.00% of the rats died within 24 h in the FHF injection group and the FHF no-loaded plasmid group, respectively, while the FHF C-sis plasmid group died only in the 24 h observation period. Compared with the blank control group, the apoptosis of liver tissue increased in the injection group of FHF Csis solution and decreased the apoptosis in the liver tissue of the control group compared with the control group. Compared with the normal control group, the levels of alt P 0.01) and ASTX P 0.05) in FHF C-sis group were significantly higher than those in control group, and those in FHF C-sis plasmid group were lower than those in control group. Conclusion C-sis gene can inhibit FHF induced by LPS combined with D-GalN in rats.
【作者单位】：南昌大学第二附属医院消化内科;
【基金】：国家自然科学基金资助项目(编号:81300348) 江西省青年科学基金资助项目(编号:20151BAB215006) 江西省教育厅科学技术研究项目(编号:GJJ150259) 江西省卫生计生委科技计划项目(编号:20165199)
【分类号】：R575.3

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