bmp-2和vegf165共表达基因修饰的BMSCs对腱骨界面愈合的研究

发布时间：2018-05-29 13:59

本文选题：血管内皮生长因子 + 骨形态发生蛋白-　；参考：《安徽医科大学学报》2017年03期

【摘要】：目的探讨骨形态发生蛋白-2(bmp-2)和血管内皮生长因子165(vegf165)共表达基因修饰的骨髓间充质干细胞(BMSCs)对兔前交叉韧带重建术后腱骨界面的组织形态学和生物力学的影响。方法将66只大白兔,随机分成实验组、对照组及正常组。实验组术后在腱骨界面注入经慢病毒转染稳定表达bmp-2和vegf165的BMSCs和纤维蛋白胶(FG),对照组腱骨界面注入BMSCs和FG,分别在术后第3、8和12周进行组织学观察和生物力学检测。结果组织学病理显示,实验组新生血管和成纤维细胞浸润高于对照组,并在术后第12周形成典型的4层直接止点结构,其组织愈合情况优于同期对照组。生物力学显示相同时间点实验组最大载负荷明显高于对照组,差异有统计学意义(P0.05)。结论 bmp-2和vegf165共表达基因修饰的BMSCs对腱骨界面愈合有促进作用。
[Abstract]:Objective to investigate the effects of bone morphogenetic protein-2mbmp-2) and vascular endothelial growth factor 165vegf165 (VEGF-165) on the histomorphology and biomechanics of tendon bone interface after anterior cruciate ligament reconstruction in rabbits. Methods 66 rabbits were randomly divided into experimental group, control group and normal group. BMSCs and fibrin glue were injected into the tendon bone interface of the experimental group after operation, and BMSCs and FG were injected into the tendon bone interface of the control group. Histological observation and biomechanical examination were performed at the 8th and 12th week after operation. Results histopathological results showed that the infiltration of neovascularization and fibroblasts in the experimental group was higher than that in the control group, and a typical 4-layer direct stop point structure was formed at the 12th week after operation, and the tissue healing was better than that in the control group. Biomechanical analysis showed that the maximum load of the experimental group was significantly higher than that of the control group at the same time, and the difference was statistically significant (P 0.05). Conclusion BMSCs modified by bmp-2 and vegf165 can promote the healing of tendon bone interface.
【作者单位】：安徽医科大学附属省立医院骨科;
【分类号】：R686.5

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