粪便细菌移植对肝性脑病大鼠肝功能及血氨的影响

发布时间：2018-06-01 06:03

  本文选题：粪便细菌移植 + 肝性脑病　； 参考：《第三军医大学学报》2017年13期

【摘要】：目的建立大鼠肝性脑病及FMT细菌模型,确定粪便细菌移植对肝性脑病大鼠肝功能及血氨的影响,初步了解粪便细菌移植(fecal microbiota transplantation,FMT)的临床意义。方法选择32只SD大鼠,采用CCL4+酒精的方法建立大鼠肝性脑病模型及肠道埋管,分为4组(A组:正常对照组;B组:肝性脑病模型组;C组:益生菌组;D组:FMT组),对C组进行益生菌移植,200μL/d(200μL益生菌溶液中含益生菌2.7×10~9),持续2周;对D组进行FMT移植,200μL/d(200μL粪便细菌溶液中含粪便细菌2.7×10~9),持续2周。记录大鼠一般生存情况及大便情况,对移植前后体重进行比较,测定移植前、后肝功能(ALT、AST、ALB、TB及DB)和门静脉及尾静脉血氨。结果所有实验大鼠均未出现死亡;粪便细菌移植后肝性脑病大鼠体重移植前明显增加[D组移植前(225.18±14.81)g,移植后2周(266.83±33.40)g,P0.05];移植后大鼠肝功能较前明显好转[移植后2周ALT:D组与B组分别为(610.49±4.98)U/L和(990.07±4.80)U/L,P0.05;AST:D组与B组分别为(72.46±4.42)U/L和(101.58±2.19)U/L,P0.05;ALB:D组与B组比较ALB明显上升,分别为(24.48±0.12)g/L和(17.53±0.53)g/L,P0.05;TB:D组为(13.45±0.77)μmol/L,B组为(27.20±0.77)μmol/L,P0.05;DB:D组为(10.04±0.09)μmol/L,B组为(16.04±0.51)μmol/L,P0.05];门静脉及尾静脉血氨较移植前明显降低[D组门静脉及尾静脉血氨分别为(26.26±0.30)μmol/L和(23.10±0.35)μmol/L,B组分别为(36.28±1.32)μmol/L和(32.90±0.35)μmol/L,P0.05]。结论对大鼠进行人体粪便细菌移植是可行的;粪便细菌移植可改善肝性脑病大鼠的肝功能,降低血氨。
[Abstract]:Objective to establish a rat model of hepatic encephalopathy and FMT bacteria, to determine the effect of fecal bacterial transplantation on liver function and blood ammonia in rats with hepatic encephalopathy, and to understand the clinical significance of fecal bacterial transplantation. Methods 32 Sprague-Dawley rats were selected to establish hepatic encephalopathy model and intestinal canal embedding by CCL4 alcohol. The rats were divided into 4 groups: normal control group, group B: liver encephalopathy model group, group C: probiotics group D: FMT group, probiotics group C containing 2. 7 脳 10 ~ 9 probiotics in probiotic solution of 200 渭 L/d(200 渭 L of probiotics for 2 weeks. Group D was transplanted with FMT for 2 weeks in a 200 渭 L/d(200 渭 L fecal bacterial solution containing 2.7 脳 10 ~ (9) of fecal bacteria. The general survival and defecation of rats were recorded and the body weight was compared before and after transplantation. Before and after transplantation, the liver function of ALTAST ALB TB and DBB) and the blood ammonia of portal vein and caudal vein were measured. Results No death was found in all the experimental rats. The body weight of rats with hepatic encephalopathy after bacterial transplantation was significantly increased [group D: 225.18 卤14.81g before transplantation, 2 weeks after transplantation: 266.83 卤33.40g / g] and liver function was improved significantly after transplantation [610.49 卤4.98)U/L in ALT:D group and 610.49 卤4.98)U/L in group B and 990.07 卤4.80U / L P0.05 AST / P 0.05 in group B at 2 weeks after transplantation] Compared with group B, ALB increased significantly in group D (72.46 卤4.42)U/L) and in group B (101.58 卤2.19U / L). 鍒嗗埆涓，

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