乙酰半胱氨酸联合地塞米松对氯气引起的急性肺损伤的保护作用

发布时间：2018-06-01 09:06

本文选题：乙酰半胱氨酸 + 地塞米松　；参考：《中国新药与临床杂志》2017年10期

【摘要】：目的研究乙酰半胱氨酸联合地塞米松对氯气引起的急性肺损伤的保护作用。方法将60只小鼠随机分为5组,每组12只。模型组、地塞米松(3 mg·kg~(-1))组、乙酰半胱氨酸(40 mg·kg~(-1))组及联合治疗(地塞米松3 mg·kg~(-1)+乙酰半胱氨酸40 mg·kg~(-1))组小鼠给予2.536 g·m-3的氯气动态染毒,染毒时间为20 min,对照组给予空气处理。建模成功后给予地塞米松组、乙酰半胱氨酸组及联合治疗组小鼠相应的药物处理,对照组和模型组给予等体积生理盐水,6 h后检测各组小鼠肺组织湿干重比和病理学、支气管肺泡灌洗液中蛋白水平和白细胞计数、肺组织MDA含量。结果与对照组相比,模型组小鼠肺组织损伤明显,肺组织损伤评分和湿干重比、支气管肺泡灌洗液中蛋白水平和白细胞计数、肺组织MDA含量均升高(P0.05)。与模型组相比,地塞米松组、乙酰半胱氨酸组和联合治疗组小鼠肺组织损伤改善,肺组织损伤评分和湿干重比、支气管肺泡灌洗液中蛋白水平和白细胞计数、肺组织MDA含量均降低(P0.05);联合治疗组与地塞米松组和乙酰半胱氨酸组相比,以上指标降低更明显(P0.05)。结论对于氯气引起的小鼠急性肺损伤,乙酰半胱氨酸联合地塞米松治疗效果优于单药治疗。
[Abstract]:Objective to study the protective effect of acetylcysteine and dexamethasone on acute lung injury induced by chlorine. Methods 60 mice were randomly divided into 5 groups with 12 mice in each group. Model group, dexamethasone (3 mg / kg) group, acetylcysteine (40 mg / kg) group and combined treatment group (dexamethasone 3 mg / kg / kg) / acetylcysteine (40 mg / kg / kg) group were given 2.536 g / m ~ (-3) chlorine for 20 min, and the control group were given air treatment. Dexamethasone group, acetylcysteine group and combined treatment group were treated with dexamethasone, and the rats in the control group and the model group were treated with the same volume of saline for 6 h. The lung wet / dry weight ratio and pathology of each group were measured. Bronchoalveolar lavage fluid protein level, white blood cell count, lung tissue MDA content. Results compared with the control group, the lung injury score and wet dry weight ratio, the protein level in bronchoalveolar lavage fluid, the white blood cell count and the MDA content of lung tissue in the model group were higher than those in the control group (P 0.05). Compared with model group, dexamethasone group, acetylcysteine group and combined treatment group improved lung tissue injury, lung injury score and wet / dry weight ratio, protein level in bronchoalveolar lavage fluid and white blood cell count. Compared with dexamethasone group and acetylcysteine group, the content of MDA in lung tissue decreased significantly (P 0.05), and that in combined treatment group was significantly lower than that in dexamethasone group and acetylcysteine group. Conclusion acetylcysteine combined with dexamethasone is more effective than single drug in the treatment of acute lung injury induced by chlorine in mice.
【作者单位】：武汉市第三医院重症医学科;
【分类号】：R563.8

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