烧伤患者金黄色葡萄球菌分离株外毒素基因研究

发布时间：2018-06-04 12:50

本文选题：金黄色葡萄球菌 + 外毒素　；参考：《中华医院感染学杂志》2016年19期

【摘要】：目的了解烧伤患者金黄色葡萄球菌(SAU)分离株的各类外毒素基因携带状况,为临床SAU感染的治疗提供依据。方法收集2015年1-6月医院烧伤患者标本分离20株SAU,通过法国生物梅里埃公司VITEK-2compact全自动鉴定仪进行菌株鉴定,PCR方法分析外毒素基因(set1-26)和(set30-40),采用Q型类进行聚类分析。结果 20株SAU经37种外毒素基因检测,只有1株未检出基因;其余19株均有阳性检出;外毒素基因中set2、set4、set16、set23、set37、set39等无阳性检出,其余阳性检出率在5.0%~95.0%;其中检出率50.0%有24种基因,75.0%有15种基因;外毒素基因检测结果具多样性,可分17种模式;聚类分析发现20株SAU可分为大、小两个家族。结论 20株SAU外毒素基因阳性率高,并可能与烧伤伤口脓疱疮和蜂窝织炎相关。
[Abstract]:Objective to investigate the status of exotoxin genes carried by Staphylococcus aureus isolates isolated from burn patients and to provide evidence for the treatment of SAU infection. Methods Twenty strains of sau were collected from burn patients in hospitals from January to June 2015. The foreign toxin gene set1-26 and set30-40 were identified by VITEK-2compact automatic identification instrument of French Bio-Meridier Company, and cluster analysis was carried out by using Q-type cluster. Results only one of the 20 SAU strains was detected by 37 exotoxin genes, while none of the other 19 strains were positive, and no positive genes were detected in the foreign toxin gene, such as set2set4set16set16set23set37set39, and so on. The remaining positive rate was 5.00.Among them, 50.0% had 24 genes and 75.0% had 15 genes, and the results of exotoxin gene detection were diverse and could be divided into 17 patterns. Cluster analysis showed that 20 strains of SAU could be divided into two families: large and small. Conclusion the positive rate of 20 strains of SAU exotoxin gene is high and may be related to burn wound pustular ulcers and cellulitis.
【作者单位】：宁波市第二医院医院感染管理科;宁波市第二医院烧伤科;宁波市第二医院医院检验科;无锡市克隆遗传技术研究所;
【基金】：浙江省医药卫生科技计划基金资助项目(2014KYA197,2015KYB343)
【分类号】：R644

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