腹腔置管引流对重症急性胰腺炎大鼠腺泡细胞内钙超载的作用

发布时间：2018-06-05 00:27

本文选题：重症急性胰腺炎 + 置管引流　；参考：《重庆医科大学》2013年硕士论文

【摘要】：目的通过对重症急性胰腺炎（Severe acute pancreatitis,SAP）模型大鼠进行腹腔置管引流，探讨其对胰腺腺泡细胞内钙超载机制的作用。方法将72只雄性Wistar大鼠随机分为假手术组、SAP组和引流组，，各24只，后两组采用“经十二指肠逆行胰胆管注射牛磺胆酸钠”法诱导SAP大鼠模型，后对引流组进行腹腔置管引流出腹水，三组大鼠分别在术后6h、12h、24h定点处死，每个时间点8只，取静脉血和腹水检测白细胞介素(Interleukin,IL)-8、肿瘤坏死因子（Tumornecrosis factor,TNF）-ɑ、血栓素（Thromboxane,Tx）A_2等炎性因子和淀粉酶（Amylase,AMY）、磷脂酶((Phospholipase,PL)A_2的活性等，取胰腺组织检测腺泡细胞内[Ca~(2+)]i和HE染色病理切片。结果引流组在6h、12h、24h三个时相点的血清IL-8、TNF-ɑ、AMY、TxA_2、胰腺组织PLA_2活性和腺泡细胞内[Ca~(2+)]i相比SAP组都有明显降低（P0.05），SAP组和引流组都呈典型的SAP病理改变,但引流组的病理评分较SAP组显著减少（P0.05）。结论对SAP大鼠进行腹腔置管,引流出IL-8、TNF-ɑ等炎性因子，降低了胰腺组织中PLA_2的活性以及血清IL-8、TNF-ɑ、TxA_2、LT等的浓度，减轻了胰腺损伤，这可能和胰腺腺泡细胞内的钙超载缓解有关。
[Abstract]:Objective to investigate the mechanism of calcium overload in pancreatic acinar cells by intraperitoneal drainage in severe acute pancreatitis (SAP) model rats with severe acute pancreatitis (SAP). Methods Seventy-two male Wistar rats were randomly divided into sham-operation group (n = 24) and drainage group (n = 24). SAP rats were induced by injection of sodium taurocholate into duodenal retrograde pancreaticobiliary duct in the latter two groups. The rats in the three groups were killed at 24 hours after the operation, 8 rats at each time point. The levels of interleukin-8, Tumornecrosis factor (Tumornecrosis factor), Thromboxane TxA2, amylase Amylase and phospholipase PLA2 were measured in venous blood and ascites. The pancreatic tissues were taken to detect the Ca~(2 in acinar cells and the histopathological sections with HE staining. Results in the drainage group, the serum IL-8 TNF-AMYYTxA2was significantly lower than that in the SAP group at three phases at 6 h, 12 h and 24 h. Compared with the SAP group, both the pancreatic tissue PLA_2 activity and the acinar cell [Ca~(2] I showed typical SAP pathological changes. However, the pathological score of the drainage group was significantly lower than that of the SAP group, but the pathological score of the drainage group was significantly lower than that of the SAP group, and the pathological score of the drainage group was significantly lower than that of the SAP group. Conclusion Intraperitoneal catheterization was performed in SAP rats, and inflammatory factors such as IL-8 TNF-T were drained out, which decreased the activity of PLA_2 and the concentration of serum IL-8 TNF-TxA2LT in pancreatic tissue, and alleviated pancreatic injury, which may be related to the relief of calcium overload in pancreatic acinar cells.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R657.51

【参考文献】