KLF4在创伤新生血管内皮细胞中的表达和对血管内皮细胞功能的影响

发布时间：2018-06-11 23:51

本文选题：皮肤创伤愈合 + 血管新生　；参考：《华中科技大学》2013年硕士论文

【摘要】：目的观察皮肤创伤愈合过程中KLF4（Kruppel-LikeFactor4）在新生血管内皮细胞中的表达及在体外对血管内皮细胞增殖、迁移及管腔形成能力的影响。方法用24只大鼠作皮肤创伤模型，分别于创伤后即刻、2、4、6、8、10、12、14d处死并取创缘组织，应用免疫组织化学技术和图像分析技术观察创伤模型大鼠在不同时间点皮肤创缘组织新生血管内皮细胞中KLF4的表达情况；在体外培养人脐静脉内皮细胞（HUVECs），，用KLF4小干扰RNA(siRNA)转染人脐静脉内皮细胞(HUVECs)48h后，应用CellCountingKit-8(CCK-8)、细胞划痕愈合实验以及体外血管形成实验分别检测转染前后HUVECs的增殖、迁移以及管腔形成能力的变化。结果与对照组相比，KLF4在肉芽组织新生血管内皮细胞中有表达；转染siRNA下调KLF4的表达后，HUVECs的增殖、迁移及管腔形成能力较对照组均明显下降，转染前HUVECs的吸光度（A）值、迁移距离和成管环数分别为1.396±0.025、497±6.2、32±0.7,转染后为0.948±0.043、206±5.6、12±0.7（P0.05）。结论本研究证实KLF4参与皮肤创伤修复过程，对皮肤创伤愈合过程中新生血管
[Abstract]:Objective to observe the expression of KLF4- Kruppel-LikeFactor4 in neovascularization endothelial cells and its effect on proliferation, migration and lumen formation of vascular endothelial cells in vitro. Methods 24 rats were used as skin trauma models. The wound tissue was killed immediately after trauma and the expression of KLF4 was observed by immunohistochemistry and image analysis at different time points in the neovascularization endothelial cells of the wound model rats. Human umbilical vein endothelial cells (HUVECs) were cultured in vitro. HUVECs were transfected with KLF4 small interference RNA-siRNAs for 48 hours. Cell counting Kit-8CCK-8 was used to detect the proliferation of HUVECs before and after transfection. Results compared with the control group, KLF4 was expressed in neovascularization endothelial cells, and the proliferation, migration and lumen formation of HUVECs were significantly decreased after transfection of siRNA down-regulated the expression of KLF4. Before transfection, the absorbance of HUVECs, the migration distance and the number of tubular rings were 1.396 卤0.025497 卤6.2, 32 卤0.7, and 0.948 卤0.043206 卤5.6, 12 卤0.7, respectively. Conclusion this study confirmed that KLF4 is involved in the repair of skin trauma and the neovascularization in the healing process of skin trauma.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R641

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