荷负电气溶胶对大鼠烧伤创面表皮干细胞的影响

发布时间：2018-06-17 09:12

本文选题：烧伤创面 + 表皮干细胞　；参考：《中国老年学杂志》2017年21期

【摘要】：目的分析大鼠烧伤创面采用荷负电气溶胶对表皮干细胞的影响并分析作用机制。方法选取清洁级健康大鼠,在背部侧分别做烧伤创面,随机分为实验组和对照组,实验组采取荷负电气溶胶治疗,对照组大鼠不做任何治疗,分析两组大鼠创面愈合过程,通过免疫组织化学染色法分析干细胞含量,并分析表皮生长因子(EGF)、β1整合素表达变化。结果烧伤24 h后取创面组织染色观察为深Ⅱ度病理改变,第2天基底层细胞增殖,5 d表皮细胞增殖,排列紧密。对照大鼠第3天局部增殖,第7天增殖明显,分布稀疏。经过治疗,相同时间实验组组织切片内阳性单位含量显著大于对照组(P0.05)。实验组平均愈合时间[(7.1±1.2)d]显著短于对照组[(9.5±1.2)d]。实验组大鼠在伤后EGF和β1整合素该表达;在伤后第3天表达明显增高,第7天仍然维持较高表达,对照组大鼠EGF和β1整合素在伤后第3天有所表达,第7天表达高峰,两组大鼠EGF和β1整合素随着治疗时间不同存在显著差异(P0.05)。结论大鼠烧伤创面实施荷负电气溶胶治疗能够促进表皮干细胞的增殖,其机制可能与EGF、β1整合素表达上升有关。
[Abstract]:Objective to investigate the effect of negative charged aerosol on epidermal stem cells (ESC) in burn wounds of rats and its mechanism. Methods the clean grade healthy rats were randomly divided into experimental group and control group. The experimental group was treated with negative charge aerosol, and the control group was not treated with any treatment. The healing process of the wound in the two groups was analyzed. The content of stem cells and the expression of EGFN and 尾 1 integrin were analyzed by immunohistochemical staining. Results after 24 hours of burn, the wound tissue was stained with deep second-degree pathological changes, and the basal layer cells proliferated on the second day, and the epidermal cells proliferated closely on the second day. The control rats proliferated locally on the 3rd day, and on the 7th day, the proliferation was obvious and the distribution was sparse. After treatment, the content of positive units in the tissue sections of the experimental group was significantly higher than that in the control group at the same time. The mean healing time of the experimental group [7.1 卤1.2 days] was significantly shorter than that of the control group [9.5 卤1.2 days]. The expression of EGF and 尾 1 integrin in the experimental group was significantly increased on the 3rd day after injury, and remained high on the 7th day, while the expression of EGF and 尾 1 integrin in the control group was increased on the 3rd day after injury, and the peak expression was observed on the 7th day after injury, and the expression of EGF and 尾 1 integrin in the control group was higher than that in the control group on the 3rd day after injury. There were significant differences in EGF and 尾 1 integrin between the two groups with different treatment time (P 0.05). Conclusion the proliferation of epidermal stem cells can be promoted by applying negative charge aerosol therapy to burn wound in rats, and the mechanism may be related to the increased expression of EGF and 尾 1 integrin.
【作者单位】：暨南大学附属第一医院烧伤整形美容科;暨南大学附属第一医院神经外科;天门市第一人民医院外科;
【基金】：国家卫生计生委医药卫生科技发展研究中心“重大疾病防治科技行动计划-创伤修复专项”课题(ZX-01-C2016021) 2014年广州市花都区科技计划项目(HD14CXY001) 2014年暨南大学第一临床医学院护理科研专项基金立项项目(2014305)
【分类号】：R644

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