依那普利拉对严重烧伤大鼠心肌损伤的保护作用及其机制探讨

发布时间：2018-06-19 20:21

  本文选题：依那普利拉 + 严重烧伤　； 参考：《安徽医科大学学报》2016年09期

【摘要】：目的探讨应用依那普利拉的早期干预对严重烧伤大鼠心肌损害的保护作用,并对其作用机制进行初步研究。方法 66只雌性清洁级SD大鼠,随机将大鼠分为对照组6只、烧伤组30只、治疗组30只。烧伤组和治疗组分为伤后1、3、6、12、24 h 5个时相点,每时相点6只大鼠。烧伤组与治疗组大鼠采用热水直接烫伤法制作严重烧伤模型,伤后予以腹腔注射补液抗休克,治疗组加用依那普利拉早期干预,均在伤后各时相点行腹主动脉采血,留取心脏组织,ELISA法检测血清心肌肌钙蛋白Ⅰ(cTnⅠ)、白介素1(IL-1)、肿瘤坏死因子α(TNF-α)、血管紧张素Ⅱ(AngⅡ)水平、心肌AngⅡ水平,部分心肌组织应用TUNEL法检测细胞凋亡情况。结果烧伤组与治疗组血清cTnI水平在伤后各时相点较对照组均有显著性升高(P0.05),治疗组各时相点血清cTnI水平升高幅度均低于烧伤组(P0.05);烧伤组与治疗组血清IL-1、TNF-α水平各时相点较对照组均有显著性升高(P0.05),治疗组各时相点血清IL-1、TNF-α水平升高幅度均低于烧伤组(P0.05);烧伤组心肌匀浆及血清中AngⅡ在伤后显著性升高(P0.05),治疗组各时相点血清及心肌匀浆中AngⅡ水平升高幅度均低于烧伤组,其中伤后1、12 h差异有统计学意义(P0.05);烧伤后心肌细胞凋亡数量在各个时相点显著增加,治疗组各个时相点凋亡数量显著少于烧伤组(P0.05)。结论严重烧伤大鼠早期即发生心肌损伤,依那普利拉对烧伤大鼠心肌损伤起保护作用,其保护作用机制可能与减少烧伤后IL-1、TNF-α、AngⅡ的释放,减少心肌细胞凋亡有关。
[Abstract]:Objective to investigate the protective effect of early intervention of enalapril on myocardial injury in severely burned rats and its mechanism. Methods 66 female SD rats of clean grade were randomly divided into control group (n = 6), burn group (n = 30) and treatment group (n = 30). The burn group and the treatment group were divided into 5 time points, 6 rats at each time point. Rats in burn group and treatment group were treated with hot water direct scalding method to make severe burn model. The rats in treatment group were treated with intraperitoneal injection of rehydration to resist shock. The treatment group was treated with early intervention of enalapril, and blood was collected from abdominal aorta at each time point after injury. The serum levels of cardiac troponin 鈪，

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