盐酸水苏碱对实验性急性脑梗死大鼠的治疗作用

发布时间：2018-06-25 03:04

  本文选题：盐酸水苏碱 + 急性脑梗死　； 参考：《中国病理生理杂志》2017年10期

【摘要】：目的:观察盐酸水苏碱对实验性急性脑梗死大鼠的治疗效果,并探讨其可能作用机制。方法:将75只大鼠随机分为5组:假手术组、脑梗死组及不同剂量盐酸水苏碱治疗组,每组各15只。盐酸水苏碱治疗组大鼠在造模完成后分别给予盐酸水苏碱10 mg/kg、20 mg/kg和40 mg/kg灌胃,每天1次,连续给药14 d。给药完成后,对各组大鼠进行神经功能缺损评分,计算脑梗塞体积,干湿重法检测脑含水量,Western blot法检测脑组织中β-catenin、cyclin D1、糖原合成酶激酶3β(GSK-3β)和p-GSK-3β的蛋白水平。结果:与脑梗死组相比,盐酸水苏碱治疗后大鼠的神经功能缺损评分明显降低,脑梗死体积与脑含水量明显降低,β-catenin、cyclin D1和p-GSK-3β的蛋白水平明显升高。结论:盐酸水苏碱对实验性大鼠急性脑梗死具有一定的疗效,其机制可能与激活了Wnt/β-catenin信号通路有关。
[Abstract]:Aim: to observe the therapeutic effect of stachydrine hydrochloride on experimental acute cerebral infarction rats and explore its possible mechanism. Methods: 75 rats were randomly divided into 5 groups: sham operation group, cerebral infarction group and stachydrine hydrochloride treatment group with 15 rats in each group. Rats in the stachydrine hydrochloride treatment group were treated with 10 mg / kg of stachydrine hydrochloride for 20 mg/kg and 40 mg/kg, respectively, once a day for 14 days. After administration, the neurological impairment scores were evaluated, the volume of cerebral infarction was calculated, the brain water content and the protein levels of 尾 -catenin cyclin D1, glycogen synthase kinase 3 尾 (GSK-3 尾) and p-GSK-3 尾 in brain tissue were measured by dry and wet weight method. Results: compared with the cerebral infarction group, the neurological impairment score, cerebral infarction volume and cerebral water content were significantly decreased, and the protein levels of 尾 -cateninine cyclin D1 and p-GSK-3 尾 were significantly increased in rats treated with stachydrine hydrochloride. Conclusion: stachydrine hydrochloride has a certain therapeutic effect on experimental acute cerebral infarction in rats, and its mechanism may be related to the activation of Wnt- 尾 -catenin signaling pathway.
【作者单位】： 新疆石河子大学医学院第一附属医院神经内科;
【基金】：兵团应用基础研究计划(No.2015AG014)
【分类号】：R743.33
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本文编号：2064251