脑肠肽Ghrelin对脓毒症大鼠小肠上皮短肽载体-1调节作用研究

发布时间：2018-06-29 02:02

本文选题：脓毒症 + 脑肠肽　；参考：《兰州大学》2013年硕士论文

【摘要】：目的探讨外源性脑肠肽Ghrelin对脓毒症大鼠小肠上皮短肽载体-1表达及功能的影响。方法雄性SD大鼠80只,随机分为4组,正常组、假手术组、脓毒症组和Ghrelin干预组,每组20只；采用盲肠结扎穿刺术模型,制模后立即给予Ghrelin静脉干预；模型建立后20h每组随机处理10只大鼠,Elisa方法检测TNF-α、IL-1β和Ghrelin水平,免疫组化、实时定量PCR和蛋白印迹分别检测PepTl分布、mRNA和蛋白表达,高效液相色谱法检测PepTl摄取功能；每组剩余10只大鼠观察生存时间(7d)。结果(1)与正常组和假手术组相比,脓毒症时小肠黏膜受损明显,血清及小肠黏膜TNF-α、IL-1β明显升高(P0.05)；小肠黏膜PepTl分布减少,PepTl mRNA表达、蛋白表达以及对底物摄取能力明显降低(P0.05)；(2)与脓毒症组比较,Ghrelin干预组大鼠小肠黏膜损伤较轻,血清及肠黏膜TNF-α、IL-1β(P0.05);小肠黏膜PepTl分布量增多,生存率、PepTl mRNA表达蛋白表达及对底物摄取能力显著升高(P0.05)；(3)正常组与假手术组各指标比较无显著差异(P0.05)。结论脓毒症时大鼠小肠上皮PepTl mRNA、蛋白表达以及PepTl在肠黏膜分布明显下降,机体在基因及蛋白水平下调了小肠上皮PepTl生物学功能；Ghrelin干预可降低炎性反应,对脓毒症小肠上皮PepTl mRNA、蛋白表达以及摄取能力均有上调作用。
[Abstract]:Objective to investigate the effects of exogenous brain gut peptide ghrelin on the expression and function of intestinal epithelial short peptide vector-1 in septic rats. Methods 80 male Sprague-Dawley rats were randomly divided into 4 groups: normal group, sham operation group, sepsis group and ghrelin intervention group with 20 rats in each group. The levels of TNF- 伪 -1 尾 and ghrelin were detected by Elisa, the mRNA and protein expression of PepTL were detected by immunohistochemistry, real-time quantitative PCR and Western blot, respectively. The uptake function of PepTL was detected by high performance liquid chromatography (HPLC). The survival time (7 days) of the remaining 10 rats in each group was observed. Results (1) compared with the normal group and the sham operation group, the intestinal mucosa was damaged significantly in sepsis, the TNF- 伪 -IL-1 尾 in serum and small intestinal mucosa was significantly increased (P0.05), the distribution of PepTl in intestinal mucosa decreased the expression of PepTl mRNA, the protein expression and the ability of uptake of substrate (P0.05). (2) compared with the sepsis group, the intestinal mucosal damage in ghrelin intervention group was lighter, TNF- 伪 -IL-1 尾 in serum and intestinal mucosa increased (P0.05), and PepTl mRNA expression and substrate uptake in small intestinal mucosa increased significantly (P0.05), while the survival rate of ghrelin intervention group was significantly higher than that in sepsis group. (3) there was no significant difference between normal group and sham operation group (P0.05). Conclusion PepTl mRNAs, protein expression and PepTl distribution in intestinal mucosa were significantly decreased in septic rats. The biological function of PepTl in intestinal epithelium was down-regulated by ghrelin intervention at gene and protein level. It can up-regulate PepTl mRNAs, protein expression and uptake ability in septic intestinal epithelium.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7

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