臭氧气浴对深Ⅱ度烧伤大鼠创面病理变化及组织细胞因子表达的影响

发布时间：2018-07-03 19:50

本文选题：烧伤 + 臭氧气浴　；参考：《中国病理生理杂志》2017年11期

【摘要】：目的:探讨臭氧气浴干预对大鼠深Ⅱ度烧伤创面的病理变化及局部组织中细胞因子血小板源性生长因子(PDGF)、转化生长因子β_3(TGF-β_3)和肿瘤坏死因子α(TNF-α)表达的影响。方法:取80只雄性清洁级SD大鼠,随机分为臭氧气浴实验组和常规换药对照组各40只。建立背部深Ⅱ度烧伤模型,伤后3 d、7 d、14 d和21d,2组创面分别取材检测。创面常规换药,对照组的创面以生理盐水清洗和碘伏油纱包扎,隔日换药1次;臭氧气浴实验组在换药前将大鼠放入清洁泡沫盒内,打开臭氧发生器开关,以输出浓度为50 mg/L的臭氧熏蒸创面20min,关闭开关,再行创面常规换药,隔日1次,直至愈合。各时点每组大鼠创面打开时取1次创面中心组织标本,然后实验组行生理盐水棉球轻拭创面和臭氧气熏蒸,对照组仅用生理盐水棉球轻拭创面后再分别取材1次。标本行HE染色组织学观察和免疫组化染色半定量观察,结合图像数据分析及ELISA法检测2组创面组织中细胞因子PDGF、TGF-β_3和TNF-α含量。结果:创面大体观察可见,臭氧气浴实验组的创面平整,边缘清晰,炎性反应轻,肿胀和渗出程度均较对照组弱,创面愈合率高于常规换药对照组,2组比较差异有统计学意义。显微镜下观察HE染色组织可见臭氧气浴实验组的创面各时点炎性反应程度比常规换药对照组轻,而新生毛细血管、成纤维细胞和上皮细胞增生数量明显优于常规换药对照组。与对照组比较,臭氧气浴实验组各时点烧伤大鼠的创面组织匀浆上清液中PDGF和TGF-β_3表达量均高于常规换药对照组,而TNF-α表达量明显低于对照组,2组比较差异均有统计学意义。结论:臭氧气浴疗法干预大鼠深Ⅱ度烧伤创面,能改善局部病理变化,促进与创面愈合相关的细胞因子PDGF和TGF-β_3表达,同时减轻炎性介质TNF-α的表达。
[Abstract]:Objective: To investigate the effects of ozone gas bath on the pathological changes of deep second degree burn wounds in rats and the effect of the expression of cell factor platelet derived growth factor (PDGF), transforming growth factor beta _3 (TGF- beta _3) and tumor necrosis factor alpha (TNF- alpha) in local tissues. Methods: 80 male clean grade SD rats were randomly divided into the ozone gas bath experimental group and the normal group. The model of deep second degree burn on the back was established in 40 control groups. The wounds were 3 D, 7 d, 14 d and 21d after injury. The wounds of the 2 groups were measured separately. The wound surface was changed by conventional medicine. The wounds in the control group were treated with saline and iodophor dressing and 1 times the other day. The ozone bath experiment group put the rats in the clean foam box before changing the drug and opened the ozone occurrence. The apparatus was switched on, with the ozone fumigation of 50 mg/L of 20min, closed switch, and then routine dressing, 1 times every other day until healing. At each time point, the wound center tissue specimens were taken for 1 times in each group of rats, and the experimental group was treated with saline cotton swab and ozone fumigation, and the control group was only wiped with saline cotton ball. 1 times after the wound, HE staining and immunohistochemical staining were observed. The contents of cytokine PDGF, TGF- beta _3 and TNF- a were detected by image data analysis and ELISA method. Results: the surface of the wound surface was clear, the edge was clear, and the inflammatory reaction was light, The swelling and exudation degree was weaker than the control group, the wound healing rate was higher than that of the conventional control group, and the 2 groups were significantly different. Under the microscope, the HE staining tissue showed that the degree of inflammatory reaction in the ozone bath experiment group was lighter than the conventional change control group, while the new blood capillary, fibroblast and epithelial cells proliferated. Compared with the control group, the expression of PDGF and TGF- beta _3 in the wound tissue supernatant of the burned rats of the ozone bath experimental group was higher than that of the conventional control group, while the expression of TNF- a was significantly lower than that of the control group, and the difference between the 2 groups was statistically significant. Conclusion: ozone bath therapy intervention was significant. Deep second degree burn wound in rats can improve the local pathological changes, promote the expression of cytokine PDGF and TGF- beta _3 related to wound healing, and reduce the expression of TNF- alpha in inflammatory mediators.
【作者单位】：武汉大学同仁医院暨武汉市第三医院烧伤研究所;
【基金】：武汉市卫计委临床医学科研项目[武卫2014(92)号WX14C21]
【分类号】：R644

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