复方苹果酸钠对失血性休克大鼠小肠黏膜细胞凋亡的影响

发布时间：2018-07-04 13:31

  本文选题：复方苹果酸钠 + 液体复苏　； 参考：《临床麻醉学杂志》2017年06期

【摘要】：目的观察复方苹果酸钠对失血性休克大鼠小肠黏膜细胞凋亡的影响。方法雄性SD大鼠48只,体重280~320g,随机分为假手术组(SS组)、生理盐水组(NS组)、复方乳酸钠组(RL组)和复方苹果酸钠组(RM组),每组12只。SS组为空白对照组,其余三组制作失血性休克模型,放血至MAP降为(40±5)mm Hg,休克60min后进行对应液体复苏,复苏后3h处死大鼠。检测小肠黏膜中B淋巴细胞瘤-2基因(Bcl-2)表达量和Bcl-2相关蛋白(Bax)含量,采用TUNEL法检测黏膜细胞凋亡情况,计算凋亡指数。检测小肠组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)浓度。取小肠黏膜做病理切片,观察病理改变。结果 NS、RL、RM组Bcl-2表达量明显低于SS组,Bax含量及凋亡指数明显高于SS组(P0.05);RM组Bcl-2表达量明显高于NS组和RL组,Bax含量及凋亡指数明显低于NS组和RL组(P0.05)。NS、RL、RM组SOD活性明显低于SS组,MDA浓度明显高于SS组(P0.05);RM组SOD活性明显高于NS组和RL组,MDA浓度明显低于NS组和RL组(P0.05)。病理组织切片显示RM组小肠黏膜损伤轻于NS组和RL组。结论复方苹果酸钠对于失血性休克大鼠小肠黏膜具有良好的抗凋亡作用,为临床晶体液的应用带来新的选择。
[Abstract]:Objective to observe the effect of compound malate sodium on apoptosis of intestinal mucosal cells in hemorrhagic shock rats. Methods Forty-eight male SD rats, weighing 280 ~ 320g, were randomly divided into three groups: sham operation group (SS group), saline group (NS group), compound sodium lactate group (RL group) and compound malate sodium group (RM group). The model of hemorrhagic shock was made in the other three groups, and the bleeding to map was reduced to (40 卤5) mm Hg. The corresponding fluid resuscitation was performed after shock and 60min, and the rats were killed 3 hours after resuscitation. The expression of B lymphocyte tumor 2 gene (Bcl-2) and Bcl-2 associated protein (Bax) in small intestinal mucosa were detected. Tunel method was used to detect the apoptosis of mucosal cells and the apoptotic index was calculated. The activity of superoxide dismutase (SOD) and the concentration of malondialdehyde (MDA) in small intestine were measured. The intestinal mucosa was taken as pathological section and the pathological changes were observed. Results the expression of Bcl-2 in RM group was significantly lower than that in SS group (P0.05). The expression of Bcl-2 in RM group was significantly higher than that in NS group and RL group, and the apoptotic index was significantly lower than that in NS group and RL group (P0.05) .NSL RLM group was significantly lower than that in NS group and RL group (P0.05). The activity of SOD in RM group was significantly lower than that in RL group (P0.05). The concentration of MDA in SS group was significantly higher than that in SS group (P0.05). The activity of SOD in RM group was significantly higher than that in NS group and RL group (P0.05). Pathological sections showed that intestinal mucosal damage in RM group was less than that in NS and RL groups. Conclusion compound sodium malate has a good antiapoptotic effect on the intestinal mucosa of hemorrhagic shock rats, which brings a new choice for the application of clinical lens fluid.
【作者单位】： 暨南大学第二临床学院深圳市麻醉医学工程研究中心;华中科技大学同济医学院附属协和医院麻醉科;
【基金】：深圳市科创委基金(JCYJ20130402092657766) 深圳市卫生科研基金(201401004)
【分类号】：R605.971
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本文编号：2096230