弥漫性脑损伤后脑水肿和c-Fos、c-Jun蛋白的变化

发布时间：2018-07-06 10:06

本文选题：弥漫性脑损伤 + 脑水肿　；参考：《泰山医学院》2013年硕士论文

【摘要】：目的研究弥漫性脑损伤后不同时间内脑水肿和c-Fos、c-Jun蛋白的变化，探讨脑损伤后的生理变化为临床治疗奠定基础。方法采用Marmarou方法制作大鼠弥漫性脑损伤模型，在损伤后不同时间（1h,3h,6h,12h,1d,3d,7d），检测脑组织含水量的变化及用免疫组织化学技术和图像分析方法观察c-Fos和c-Jun的表达规律。结果伤后脑组织含水量，在1h即有所提高，1d达高峰，，至7d仍高于对照组水平。c-Fos和c-Jun在伤后1h即可见到大量表达；c-Fos在伤后3h达高峰（P0.05），随后有所降低，至3d再次达到高峰（P0.05）,7d明显降低但仍高于对照组（P0.05）。c-Jun阳性细胞数在伤后6h达高峰（P0.05），随后有所降低，至3d又达高峰（P0.05）,7d明显降低但仍高于对照组（P0.05）。结论弥漫性脑损伤后脑组织含水量和c-Fos/c-Jun的表达呈现出不同的规律性。
[Abstract]:Objective to study the changes of brain edema and c-Fosson-c-Jun protein in different time after diffuse brain injury, and to explore the physiological changes of brain injury in order to lay a foundation for clinical treatment. Methods the rat model of diffuse brain injury was established by Marmarou method. The changes of water content in brain tissue and the expression of c-Fos and c-Jun were observed by immunohistochemistry and image analysis at different time after injury (1 h ~ 3 h ~ 6 h ~ 12 h ~ 1 d ~ 3 d ~ (-1). Results the water content of brain tissue increased to the peak at 1 hour after injury, and reached the peak at 7 days after injury. The expression of c-Fos and c-Jun reached its peak at 3 hours after injury (P0.05), and then decreased. The number of c-Jun positive cells reached the peak at the 3rd day (P0.05) but still higher than that in the control group (P0.05). The number of c-Jun positive cells reached the peak at 6h after injury (P0.05), then decreased to the peak (P0.05) on the 3rd day (P0.05), but still higher than that of the control group (P0.05). Conclusion the water content of brain tissue and the expression of c Fos / c Jun in brain tissue after diffuse brain injury have different regularity.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R651.15

【参考文献】