亚低温对心肺复苏后心肌线粒体氧化应激的影响

发布时间：2018-07-12 12:07

本文选题：线粒体 + 氧化应激　；参考：《广东医学》2016年20期

【摘要】：目的研究亚低温对心肺复苏后心肌线粒体氧化应激的影响。方法选取24只健康的成年新西兰大白兔,雌雄不拘。将这24只白兔随机分为室温组和低温组,每组12只。室温组自主循环恢复后置于室温状态下观察12 h。低温组经腹腔诱导亚低温下观察12 h。记录结果,建立模型后分别检测相关数据进行分析比较。结果对两组动物心肺复苏各项指标进行比较,低温组经腹腔诱导亚低温后心肺复苏的效果具有明显优势,差异有统计学意义(P0.05)。对两组动物心肌细胞线粒体内的活性氧、超氧化物歧化酶、丙二醛等氧化应激指标进行比较,发现低温组的氧化应激指标含量均低于室温组,差异有统计学意义(P0.05)。低温组心肌细胞的线粒体肿胀度、线粒体膜的流动性及电镜下心肌细胞线粒体超显微形态结构较室温组好,差异有统计学意义(P0.05)。结论亚低温下心肌细胞及线粒体内氧化应激反应都能得到有效的抑制,能够维护再灌注后的心功能。
[Abstract]:Objective to study the effect of mild hypothermia on oxidative stress of myocardial mitochondria after cardiopulmonary resuscitation (CPR). Methods 24 healthy adult New Zealand white rabbits were selected. The 24 rabbits were randomly divided into room temperature group (n = 12) and hypothermia group (n = 12). The rats in room temperature group were observed at room temperature for 12 hours after recovery of autonomic circulation. The hypothermia group was induced by abdominal cavity for 12 hours. The results were recorded, and the relevant data were analyzed and compared after the establishment of the model. Results the indexes of cardiopulmonary resuscitation in the two groups were compared. The effect of mild hypothermia induced cardiopulmonary resuscitation in hypothermia group was significant (P0.05). The contents of reactive oxygen species, superoxide dismutase and malondialdehyde in mitochondria of two groups were compared. The results showed that the content of oxidative stress index in hypothermia group was lower than that in room temperature group, the difference was statistically significant (P0.05). The degree of mitochondria swelling, the fluidity of mitochondrial membrane and the ultrastructure of myocardial mitochondria under electron microscope in hypothermia group were better than those in room temperature group (P0.05). Conclusion oxidative stress in myocardial cells and mitochondria can be effectively inhibited under mild hypothermia and can maintain cardiac function after reperfusion.
【作者单位】：广东省广州市第十二人民医院心血管内科;中山大学附属第一医院急诊科;广东省广州市第十二人民医院急诊科;广东省广州市第十二人民医院ICU科;
【基金】：广东省医学科研基金资助项目(编号:A2014595)
【分类号】：R459.7

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