小鼠烧伤后免疫细胞多功能紊乱的基因表达谱生物信息学分析

发布时间：2018-07-13 13:43

【摘要】：目的探讨小鼠烧伤后外周血免疫细胞基因表达差异,并对差异基因行生物信息分析,从分子水平全面探讨烧伤后免疫细胞功能变化情况,为临床治疗提供新靶点。方法在GEO数据库中下载基因芯片数据(GSE7404,小鼠,25%TBSA,3度),经过芯片质量评估后,获取差异基因,分别应用go-function数据集及DAVID Bioinformatics Resources 6.7对差异基因进行基因本体、生物学通路分析,获得差异基因相关功能的功能富集类。结果在烧伤后第1天筛选到1 825个差异基因,其中上调基因658个,下调基因1 167个。Gene Ontology分析显示小鼠烧伤后免疫细胞与免疫系统过程、细胞过程、代谢过程、应对刺激反应、生物调节及死亡等功能高度相关。KEGG通路分析显示上调基因主要富集到免疫系统、细胞生长与死亡及代谢相关通路;而下调基因则主要与免疫及代谢通路相关。结论烧伤是一个复杂的病理生理过程,会引起多种基因表达调控的改变。基因芯片表达谱的生物信息学分析可以在转录水平反映免疫细胞在烧伤后的分子生物学过程,有助于全面认识烧伤后免疫细胞功能紊乱的发生机制。
[Abstract]:Objective to investigate the difference of gene expression in peripheral blood immunocytes after burn in mice, and to analyze the biological information of the differentially expressed genes, so as to explore the changes of immune cell function at the molecular level in order to provide a new target for clinical treatment. Methods GeneChip data (GSE7404, mouse 25 TBSAA 3 degrees) were downloaded from GEO database. After chip quality assessment, differentially expressed genes were obtained. Gene ontology and biological pathway analysis were performed by using go-function dataset and david Bioinformatics Resources 6.7, respectively. Functional enrichment classes of differential gene related functions were obtained. Results on the first day after burn, 1 825 differentially expressed genes were screened, including 658 up-regulated genes, and 11167 down-regulated genes. Gene Ontology analysis showed that the immune cells and immune system, cellular processes, metabolic processes and response to stimuli were observed in mice after burn. KEGG pathway analysis showed that the up-regulated gene was mainly enriched into the immune system, cell growth was related to death and metabolism, while down-regulated gene was mainly related to immune and metabolic pathway. Conclusion Burn is a complex pathophysiological process, which can change the regulation of gene expression. Bioinformatics analysis of gene microarray expression profile can reflect the molecular biological process of immune cells after burn at the transcriptional level, which is helpful to fully understand the mechanism of immune cell dysfunction after burn.
【作者单位】：南方医科大学南方医院烧伤科;
【基金】：广东省科技计划项目(编号:2014A020212189) 南方医科大学南方医院院长基金(编号:2014B013)
【分类号】：R644

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