不同浓度的高渗钠盐复苏液对严重烫伤大鼠肠损伤的影响
发布时间：2018-07-17 04:18
【摘要】：一.研究目的本研究通过观察200mmol/L、300mmol/L与400mmol/L等不同浓度的高渗钠盐液(HS)和乳酸钠林格液(LR)对严重烫伤大鼠液体复苏的效果,并从炎症因子、氧化应激反应和信号转导通道等方面探讨不同浓度的高渗钠盐复苏液对严重烫伤大鼠肠损伤影响的初步机制。二.研究方法健康成年SD大鼠104只(购自安徽医科大学实验动物中心),雌雄不限,体重在200-250g。大鼠适应性饲养一周后随机分成五组:正常对照组(8只)、乳酸钠林格液组(LR组,24只)、200mmol/L高渗钠盐液组(HS200组,24只)、300mmol/L高渗钠盐液组(HS300组,24只)、400mmol/L高渗钠盐液组(HS400组,24只)。所有大鼠均尾静脉置管,剃除背部毛发,对照组的大鼠不予烫伤和补液,LR组与各HS组大鼠背部经98℃水烫伤12s造成30%体表面积III度烧伤。烫伤后分别采用LR、200 mmol/L HS、300mmol/L HS与400mmol/L HS进行复苏。LR组与各HS组的大鼠均在伤后2h、8h和24h处死取血和肠组织。第一部分:不同浓度的高渗钠盐复苏液对严重烫伤大鼠血钠、肠含水量以及炎性细胞因子表达的影响将抽取的血标本用生化分析仪测量血清钠浓度;分别称取对照组、LR组、HS200组、HS300组与HS400组的大鼠的肠湿重(W)与干重(D),并计算肠含水量(以“湿重/干重”表示);使用酶联免疫吸附法(ELISA)测量血浆中肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和高迁移率蛋白B1(HMGB1)含量。将取得数据进行统计分析。第二部分:不同浓度的高渗钠盐复苏液对严重烫伤大鼠肠道氧化应激反应以及肠血管内皮细胞的影响取对照组、LR组、HS200组、HS300组与HS400组大鼠的一部分肠组织进行丙二醛(MDA)含量、超氧化物歧化酶(SOD)和二胺氧化酶(DAO)活性等的测定。另一部分肠组织在使用10%甲醛固定后使用免疫组化法测定血管性血友病因子(v WF)的表达。第三部分:不同浓度的高渗钠盐复苏液对严重烫伤大鼠肠组织p38MAPK以及JNK通道的影响采用蛋白质印迹法(Western Blot)测定五组大鼠肠组织中p38MAPK以及JNK通道的活性并进行统计学分析。三.结果第一部分LR组血钠浓度各时间点明显低于HS200组、HS300组与HS400组和对照组,差异具有统计学意义(P0.01)。与对照组血钠浓度相比,HS200组在伤后2h、24h增高(P0.05),在伤后8h无明显差异(P0.05),HS300组和HS400组在各时间点均明显增高,差异具有统计学意义(P0.01)。与HS200组相比,HS300组血钠浓度各时间点无明显差异(P0.05),HS400组血钠浓度各时间点均明显增高,差异具有统计学意义(P0.01)。与HS300组相比,HS400组仅在伤后24h显著增高(P0.05),其他时间点无统计学差异。LR组和HS200组在各时间点的肠湿干重比(W/D)均高于对照组,差异具有统计学意义(P0.05或P0.01),HS300组的伤后8h、24h以及HS400组的伤后8h的W/D明显高于对照组(P0.05或P0.01),其他时间点无明显统计学差异。与LR组的肠W/D相比,HS200组和HS300组在伤后2h、8h明显降低(P0.05或P0.01),伤后24h无统计学差异,HS400组在各时间点均明显降低,差异具有统计学意义(P0.05或P0.01)。HS200组、HS300组和HS400组之间的肠W/D无明显差异(P0.05)。与对照组相比,LR组的各时间点的血TNF-α和IL-1β含量均显著增高,差异具有统计学意义(P0.05或P0.01);HS200组和HS300组在伤后2h、8h以及HS400组的伤后8h的血TNF-α含量均显著增高(P0.05或P0.01);三个HS组的各时间点的IL-1β含量均明显增高,差异具有统计学意义(P0.01);LR组、HS200组和HS300组在伤后8h、24h以及HS400组的伤后24h的血HMGB1含量均显著增高,差异具有统计学意义(P0.05或P0.01),它们在伤后2h的血HMGB1含量均无统计学差异。与LR组相比,HS200组、HS300组与HS400组在伤后2h、8h的血TNF-α含量均明显降低,差异具有统计学意义(P0.01);它们在各时间点的血IL-1β含量均显著减低(P0.01),它们在伤后8h和24h血中HMGB1含量均明显降低,差异具有统计学意义(P0.01);它们在伤后24h的血TNF-α含量以及在伤后2h的血HMGB1含量均无明显差异(P0.05)。除HS400组在伤后24h的血HMGB1的含量明显高于HS200组(P0.05)外,三个HS组在各时间点的血TNF-α、IL-1β、血HMGB1的含量均无明显差异(P0.05)。第二部分LR组、HS200组、HS300组与HS400组在各时间点的肠组织MDA含量均高于对照组,差异具有统计学意义(P0.05或P0.01)。与LR组的肠组织MDA含量相比,HS300组与HS400组在各时间点均显著降低,HS200组在伤后2h、8h明显减少,差异具有统计学意义(P0.05或P0.01)。与HS200组相比,HS300组在各时间点的肠组织MDA含量无明显差异(P0.05),HS400组在各时间点的肠组织MDA含量均明显降低(P0.05或P0.01);与HS300组相比,HS400组在各时间点的肠组织MDA含量无统计学差异(P0.05)。与对照组的肠组织SOD活性相比,LR组各时间点、HS200组和HS300组在伤后8h均明显增大,差异具有统计学意义(P0.05或P0.01)。HS300组和HS400组在伤后8h的肠组织SOD活性显著低于LR组,差异具有统计学意义(P0.05或P0.01)。HS200组、HS300组和HS400组之间在各时间点的肠组织SOD活性无明显差异(P0.05)。LR组、HS200组、HS300组与HS400组的肠组织DAO活性均低于对照组,差异具有统计学意义(P0.05或P0.01)。与LR组的肠组织DAO活性相比,HS200组在各时间点无统计学差异,HS300组在伤后2h、24h以及HS400组在各时间点均显著增高,差异具有统计学意义(P0.05或P0.01)。除HS400组和HS300组在伤后2h的DAO活性比HS200组增高(P0.05或P0.01)外,三个HS组之间在伤后其他时间点的DAO活性均无明显差异(P0.05)。肠组织v WF免疫组化结果显示:与LR组比较,HS200组、HS300组与HS400组在各时间点的肠组织v WF表达有不同程度的增强,且随着HS浓度的增加而更明显。第三部分与对照组相比,LR组和HS200组肠组织p38MAPK的活性在各时间点均明显增强(P0.05或P0.01),HS300组在伤后8h、24h增强,HS400组在伤后8h增强,差异具有统计学意义(P0.01)。与LR组相比,HS200组、HS300组与HS400组肠组织p38MAPK的活性在各时间点均明显降低,差异具有统计学意义(P0.01)。HS400组在各时间点的肠组织p38MAPK活性均显著低于HS200组,在伤后2h、8h明显低于HS300组,差异具有统计学意义(P0.01)。HS300组在伤后8h、24h的肠组织p38MAPK活性均明显低于HS200组,差异具有统计学意义(P0.01)。与对照组相比,LR组在伤后各时间点、HS200组在伤后8h、24h以及HS300组和HS400组的肠组织JNK活性在伤后8h均显著增强,差异具有统计学意义(P0.01)。采用三组HS复苏,在各时间点的肠组织JNK活性均明显低于LR组,差异具有统计学意义(P0.05或P0.01)。与HS200组的肠组织JNK活性比较,HS300组在HS伤后24h明显降低,HS400组在伤后8h、24h明显下降,差异具有统计学意义(P0.01);与HS300组比较,HS400组在伤后2h、8h的肠组织JNK活性明显降低,差异具有统计学意义(P0.05或P0.01)。四.结论1.三组高渗钠盐复苏液与LR比较,均能防止低钠血症的发生,减轻严重烫伤大鼠伤后的肠道水肿并能抑制相关炎性因子的表达,三组作用上无明显差异。但是,使用400mmol/L的HS进行液体复苏,其血钠浓度明显增高。2.三组高渗钠盐复苏液与LR比较,均能减轻严重烫伤大鼠伤后肠道的氧化应激反应,可以有效保护肠血管内皮细胞,其中使用400mmol/L的HS复苏的效果最好。3.三组高渗钠盐复苏液与LR比较,均能降低严重烫伤大鼠伤后肠组织中p38MAPK和JNK的活性表达,其中使用400mmol/L的HS复苏的效果最好。
[Abstract]:The purpose of this study is to investigate the effect of high osmotic sodium salt solution (HS) and sodium lactate (LR) on the fluid resuscitation of severely scalded rats by observing the different concentrations of 200mmol/L, 300mmol/L and 400mmol/L, and to discuss the severe scald with different concentrations of hypertonic sodium salt resuscitation from the inflammatory factors, oxidative stress reactions and signal transduction pathways. The preliminary mechanism of the effect of intestinal injury in rats. Two. 104 healthy adult SD rats (purchased from the experimental animal center of Medical University Of Anhui) were randomly divided into five groups: normal control group (8 rats), sodium lactate solution group (group LR, 24), and 200mmol/L hypertonic sodium salt solution group (group HS200, 2). 4, 300mmol/L hypertonic sodium salt group (group HS300, 24), 400mmol/L hypertonic sodium salt solution group (group HS400, 24). All rats were treated with tail vein, shaving the back hair, the rats in the control group were not scalded and rehydration, and the back of the LR group and each HS group were scalded at 98 degrees centigrade to 30% body surface area III degree burn. LR, 200 mmol/ were used after the scald, respectively. L HS, 300mmol/L HS and 400mmol/L HS were used to resuscitate the.LR group and the rats of each HS group. The blood and intestinal tissue were killed in 2H, 8h and 24h after injury. Part 1: the effects of different concentrations of hypertonic sodium salt resuscitation on blood sodium, intestinal water content and expression of inflammatory cytokines in severely scalded rats were measured by biochemical analyzer. The concentration of sodium was called the intestinal wet weight (W) and dry weight (D) of the rats in the control group, the LR group, the HS200 group, the HS300 group and the HS400 group, and the calculation of the intestinal water content ("wet weight / dry weight"), and the measurement of the tumor necrosis factor alpha (TNF- a) in plasma with enzyme linked immunosorbent assay (ELISA), the content of interleukin 1 beta (IL-1 beta) and high mobility protein B1 (HMGB1). Statistical analysis of the data. The second part: the effect of different concentrations of hypertonic sodium salt resuscitation on the intestinal oxidative stress reaction and intestinal vascular endothelial cells in severely scalded rats, group LR, HS200, group HS300 and part of the rats of group HS400, the content of malondialdehyde (MDA), superoxide dismutase (SOD) and the oxidation of amines The determination of enzyme (DAO) activity. The other part of the intestinal tissue was immobilized with 10% formaldehyde to determine the expression of vascular hemophilia factor (V WF). The third part: the effect of different concentrations of hypertonic sodium salt resuscitation on the intestinal tissue p38MAPK and JNK channel in severely scalded rats was determined by western blot (Western Blot) five The activity of p38MAPK and JNK channel in the intestinal tissue of group rats was statistically analyzed. Three. Results the blood sodium concentration in group LR was significantly lower than that in group HS200. The difference between group HS300 and HS400 group and control group was statistically significant (P0.01). Compared with the concentration of sodium in the control group, HS200 group was 2h, 24h increased (P0.05) after injury, and 8h was no longer after injury. Significant difference (P0.05), group HS300 and HS400 groups were significantly higher at all time points, the difference was statistically significant (P0.01). Compared with the HS200 group, there was no significant difference in the time points of the concentration of sodium in the HS300 group (P0.05), and the concentration of sodium in the HS400 group increased significantly at each time point, and the difference was statistically significant (P0.01). Compared with the HS300 group, the HS400 group was only after the injury. 24h was significantly higher (P0.05). There was no statistical difference at other time points in.LR group and HS200 group at all time points (W/D). The difference was statistically significant (P0.05 or P0.01). The 8h in 8h, 24h and HS400 group after injury of HS300 group was significantly higher than that of the control group. There was no significant statistics at other time points. Difference. Compared with W/D in group LR, 2h, 8h decreased significantly after injury in group HS200 and HS300 (P0.05 or P0.01), and there was no significant difference in 24h after injury. The HS400 group decreased significantly at all time points, and the difference was statistically significant (P0.05 or P0.01), and there was no significant difference between the group and the group. The content of TNF- and IL-1 beta in the blood of the time point increased significantly, and the difference was statistically significant (P0.05 or P0.01), and the content of TNF- alpha in the 8h of 2h, 8h and HS400 groups after injury in HS200 and HS300 groups increased significantly (P0.05 or P0.01); the difference was statistically significant in each time point of the three groups. The serum HMGB1 content of 24h in 8h, 24h and HS400 groups after injury in group HS200 and group HS300 increased significantly (P0.05 or P0.01), and there was no statistical difference in the HMGB1 content of blood in 2h after injury. The content of blood IL-1 beta in all time points decreased significantly (P0.01), and their HMGB1 content in 8h and 24h blood decreased significantly after injury (P0.01), and there was no significant difference between the serum TNF- alpha content of 24h and the HMGB1 content of 2h in 2h after injury (P0.05). The content of MGB1 was significantly higher than that of group HS200 (P0.05). There was no significant difference in the content of TNF- alpha, IL-1 beta, and blood HMGB1 in the three HS groups at all time points (P0.05). The contents of the intestinal tissue in the second part of the LR group, HS200 group, HS300 group and HS400 group were higher than those of the control group. The content of A in group HS300 and group HS400 decreased significantly at all time points, and in group HS200, 2h, 8h decreased significantly after injury. The difference was statistically significant (P0.05 or P0.01). Compared with the HS200 group, there was no significant difference in the MDA content of intestinal tissue in the HS300 group at all time points (P0.05). 1): compared with the HS300 group, there was no significant difference in intestinal tissue MDA content in group HS400 at all time points (P0.05). Compared with the SOD activity of the intestinal tissue in the control group, the 8h of HS200 group and HS300 group increased significantly at each time point in group LR and HS300 group, and the difference was statistically significant (P0.05 or P0.01) and the activity of intestinal tissue was significantly lower in the.HS300 group and in the group after injury. In group LR, the difference was statistically significant (P0.05 or P0.01) group.HS200, and there was no significant difference in intestinal tissue SOD activity between HS300 and HS400 groups at all time points (P0.05).LR group, HS200 group, HS300 group and HS400 group were lower than the control group, and the difference has statistical significance. There was no statistical difference at all time points in group S200, and in group HS300, 2h, 24h and HS400 were significantly higher in all time points after injury. The difference was statistically significant (P0.05 or P0.01). Except for HS400 and HS300 group, DAO activity of 2H was higher than that of HS200 group. There was no obvious activity between the three groups at other time points after injury. Difference (P0.05). The immunohistochemical results of intestinal tissue V WF showed that: compared with the LR group, the expression of V WF in the intestinal tissue of the HS200 group, the HS300 group and the HS400 group increased in varying degrees, and was more obvious with the increase of HS concentration. The third part, compared with the control group, increased significantly at all time points in the LR group and the HS200 group. P0.05 or P0.01), group HS300 was 8h, 24h increased after injury, and 8h increased in group HS400 after injury. The difference was statistically significant (P0.01). Compared with group LR, the activity of HS200 group, HS300 group and HS400 group decreased significantly at all time points. Lower than HS200 group, 2h, 8h significantly lower than group HS300 after injury, the difference was statistically significant (P0.01).HS300 group 8h, 24h in the intestinal p38MAPK activity was significantly lower than the HS200 group, the difference was statistically significant (P0.01). The activity of NK increased significantly after injury (8h). The difference was statistically significant (P0.01). The activity of JNK in the intestinal tissue at all time points was significantly lower than that in the LR group with three groups of HS resuscitation. The difference was statistically significant (P0.05 or P0.01). The HS300 group decreased obviously after the HS injury. The difference was statistically significant (P0.01). Compared with the HS300 group, the activity of JNK in the intestinal tissue of group HS400 after injury was significantly reduced, and the difference was statistically significant (P0.05 or P0.01). Four. Conclusion 1. the three groups of hypertonic sodium salt resuscitation could prevent the occurrence of hyponatremia and reduce the intestinal edema after severe scald injury and inhibit the intestinal edema in severely scalded rats. There was no significant difference in the expression of related inflammatory factors in the three groups. However, 400mmol/L HS was used to resuscitate the liquid, and the serum sodium concentration was significantly higher than that of the.2. three group of hypertonic sodium salt resuscitation, which could reduce the oxidative stress reaction in the intestinal tract after severe scald injury in rats, and could effectively protect the intestinal vascular endothelial cells, which used 400mmol/L. The best effect of HS resuscitation is.3. three hypertonic sodium salt resuscitation and LR, which can reduce the expression of p38MAPK and JNK in the intestinal tissue of severely scalded rats, and HS resuscitation with 400mmol/L is the best.
【学位授予单位】：安徽医科大学
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R644
[Abstract]:The purpose of this study is to investigate the effect of high osmotic sodium salt solution (HS) and sodium lactate (LR) on the fluid resuscitation of severely scalded rats by observing the different concentrations of 200mmol/L, 300mmol/L and 400mmol/L, and to discuss the severe scald with different concentrations of hypertonic sodium salt resuscitation from the inflammatory factors, oxidative stress reactions and signal transduction pathways. The preliminary mechanism of the effect of intestinal injury in rats. Two. 104 healthy adult SD rats (purchased from the experimental animal center of Medical University Of Anhui) were randomly divided into five groups: normal control group (8 rats), sodium lactate solution group (group LR, 24), and 200mmol/L hypertonic sodium salt solution group (group HS200, 2). 4, 300mmol/L hypertonic sodium salt group (group HS300, 24), 400mmol/L hypertonic sodium salt solution group (group HS400, 24). All rats were treated with tail vein, shaving the back hair, the rats in the control group were not scalded and rehydration, and the back of the LR group and each HS group were scalded at 98 degrees centigrade to 30% body surface area III degree burn. LR, 200 mmol/ were used after the scald, respectively. L HS, 300mmol/L HS and 400mmol/L HS were used to resuscitate the.LR group and the rats of each HS group. The blood and intestinal tissue were killed in 2H, 8h and 24h after injury. Part 1: the effects of different concentrations of hypertonic sodium salt resuscitation on blood sodium, intestinal water content and expression of inflammatory cytokines in severely scalded rats were measured by biochemical analyzer. The concentration of sodium was called the intestinal wet weight (W) and dry weight (D) of the rats in the control group, the LR group, the HS200 group, the HS300 group and the HS400 group, and the calculation of the intestinal water content ("wet weight / dry weight"), and the measurement of the tumor necrosis factor alpha (TNF- a) in plasma with enzyme linked immunosorbent assay (ELISA), the content of interleukin 1 beta (IL-1 beta) and high mobility protein B1 (HMGB1). Statistical analysis of the data. The second part: the effect of different concentrations of hypertonic sodium salt resuscitation on the intestinal oxidative stress reaction and intestinal vascular endothelial cells in severely scalded rats, group LR, HS200, group HS300 and part of the rats of group HS400, the content of malondialdehyde (MDA), superoxide dismutase (SOD) and the oxidation of amines The determination of enzyme (DAO) activity. The other part of the intestinal tissue was immobilized with 10% formaldehyde to determine the expression of vascular hemophilia factor (V WF). The third part: the effect of different concentrations of hypertonic sodium salt resuscitation on the intestinal tissue p38MAPK and JNK channel in severely scalded rats was determined by western blot (Western Blot) five The activity of p38MAPK and JNK channel in the intestinal tissue of group rats was statistically analyzed. Three. Results the blood sodium concentration in group LR was significantly lower than that in group HS200. The difference between group HS300 and HS400 group and control group was statistically significant (P0.01). Compared with the concentration of sodium in the control group, HS200 group was 2h, 24h increased (P0.05) after injury, and 8h was no longer after injury. Significant difference (P0.05), group HS300 and HS400 groups were significantly higher at all time points, the difference was statistically significant (P0.01). Compared with the HS200 group, there was no significant difference in the time points of the concentration of sodium in the HS300 group (P0.05), and the concentration of sodium in the HS400 group increased significantly at each time point, and the difference was statistically significant (P0.01). Compared with the HS300 group, the HS400 group was only after the injury. 24h was significantly higher (P0.05). There was no statistical difference at other time points in.LR group and HS200 group at all time points (W/D). The difference was statistically significant (P0.05 or P0.01). The 8h in 8h, 24h and HS400 group after injury of HS300 group was significantly higher than that of the control group. There was no significant statistics at other time points. Difference. Compared with W/D in group LR, 2h, 8h decreased significantly after injury in group HS200 and HS300 (P0.05 or P0.01), and there was no significant difference in 24h after injury. The HS400 group decreased significantly at all time points, and the difference was statistically significant (P0.05 or P0.01), and there was no significant difference between the group and the group. The content of TNF- and IL-1 beta in the blood of the time point increased significantly, and the difference was statistically significant (P0.05 or P0.01), and the content of TNF- alpha in the 8h of 2h, 8h and HS400 groups after injury in HS200 and HS300 groups increased significantly (P0.05 or P0.01); the difference was statistically significant in each time point of the three groups. The serum HMGB1 content of 24h in 8h, 24h and HS400 groups after injury in group HS200 and group HS300 increased significantly (P0.05 or P0.01), and there was no statistical difference in the HMGB1 content of blood in 2h after injury. The content of blood IL-1 beta in all time points decreased significantly (P0.01), and their HMGB1 content in 8h and 24h blood decreased significantly after injury (P0.01), and there was no significant difference between the serum TNF- alpha content of 24h and the HMGB1 content of 2h in 2h after injury (P0.05). The content of MGB1 was significantly higher than that of group HS200 (P0.05). There was no significant difference in the content of TNF- alpha, IL-1 beta, and blood HMGB1 in the three HS groups at all time points (P0.05). The contents of the intestinal tissue in the second part of the LR group, HS200 group, HS300 group and HS400 group were higher than those of the control group. The content of A in group HS300 and group HS400 decreased significantly at all time points, and in group HS200, 2h, 8h decreased significantly after injury. The difference was statistically significant (P0.05 or P0.01). Compared with the HS200 group, there was no significant difference in the MDA content of intestinal tissue in the HS300 group at all time points (P0.05). 1): compared with the HS300 group, there was no significant difference in intestinal tissue MDA content in group HS400 at all time points (P0.05). Compared with the SOD activity of the intestinal tissue in the control group, the 8h of HS200 group and HS300 group increased significantly at each time point in group LR and HS300 group, and the difference was statistically significant (P0.05 or P0.01) and the activity of intestinal tissue was significantly lower in the.HS300 group and in the group after injury. In group LR, the difference was statistically significant (P0.05 or P0.01) group.HS200, and there was no significant difference in intestinal tissue SOD activity between HS300 and HS400 groups at all time points (P0.05).LR group, HS200 group, HS300 group and HS400 group were lower than the control group, and the difference has statistical significance. There was no statistical difference at all time points in group S200, and in group HS300, 2h, 24h and HS400 were significantly higher in all time points after injury. The difference was statistically significant (P0.05 or P0.01). Except for HS400 and HS300 group, DAO activity of 2H was higher than that of HS200 group. There was no obvious activity between the three groups at other time points after injury. Difference (P0.05). The immunohistochemical results of intestinal tissue V WF showed that: compared with the LR group, the expression of V WF in the intestinal tissue of the HS200 group, the HS300 group and the HS400 group increased in varying degrees, and was more obvious with the increase of HS concentration. The third part, compared with the control group, increased significantly at all time points in the LR group and the HS200 group. P0.05 or P0.01), group HS300 was 8h, 24h increased after injury, and 8h increased in group HS400 after injury. The difference was statistically significant (P0.01). Compared with group LR, the activity of HS200 group, HS300 group and HS400 group decreased significantly at all time points. Lower than HS200 group, 2h, 8h significantly lower than group HS300 after injury, the difference was statistically significant (P0.01).HS300 group 8h, 24h in the intestinal p38MAPK activity was significantly lower than the HS200 group, the difference was statistically significant (P0.01). The activity of NK increased significantly after injury (8h). The difference was statistically significant (P0.01). The activity of JNK in the intestinal tissue at all time points was significantly lower than that in the LR group with three groups of HS resuscitation. The difference was statistically significant (P0.05 or P0.01). The HS300 group decreased obviously after the HS injury. The difference was statistically significant (P0.01). Compared with the HS300 group, the activity of JNK in the intestinal tissue of group HS400 after injury was significantly reduced, and the difference was statistically significant (P0.05 or P0.01). Four. Conclusion 1. the three groups of hypertonic sodium salt resuscitation could prevent the occurrence of hyponatremia and reduce the intestinal edema after severe scald injury and inhibit the intestinal edema in severely scalded rats. There was no significant difference in the expression of related inflammatory factors in the three groups. However, 400mmol/L HS was used to resuscitate the liquid, and the serum sodium concentration was significantly higher than that of the.2. three group of hypertonic sodium salt resuscitation, which could reduce the oxidative stress reaction in the intestinal tract after severe scald injury in rats, and could effectively protect the intestinal vascular endothelial cells, which used 400mmol/L. The best effect of HS resuscitation is.3. three hypertonic sodium salt resuscitation and LR, which can reduce the expression of p38MAPK and JNK in the intestinal tissue of severely scalded rats, and HS resuscitation with 400mmol/L is the best.
【学位授予单位】：安徽医科大学
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R644
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