CGRP受体拮抗剂CGRP8-37对颅脑创伤后大鼠保护作用的实验研究

发布时间：2018-07-17 16:23

【摘要】：目的探讨脑室给予CGRP受体拮抗剂CGRP8-37对颅脑创伤后大鼠的保护作用。方法将90只Wistar大鼠随机分为对照组(6只)、创伤组(TBI组,42只)、CGRP8-37组(42只)。TBI组、CGRP8-37组大鼠分别作自由落体创伤模型,应用免疫组化和PCR技术,动态观察对照、创伤和CGRP8-37组大鼠0.5h,2h,6h,12h,24h,48h,72h后CGRP的变化规律。结果①免疫组化显示:对照组在TBI组脑组织CGRP阳性单位在伤后0.5h开始表达上调,2h、6h依次增高,12h达到峰值(P0.05),之后逐渐回落,72h时高于正常水平。与TBI组相比,CGRP8-37组脑组织CGRP阳性单位表达在2h即达到高峰,随后持续回落,48h即恢复正常,72h低于正常水平。且在相应的时间点CGRP阳性单位表达都低于TBI组。②PCR显示:与免疫组化结果基本相似,即CGRP8-37组脑组织CGRP mRNA表达较TBI组峰值提前,由12h提前至2h,且在相应时间点减少CGRP mRNA表达。结论CGRP受体拮抗剂CGRP8-37能减轻大鼠创伤性脑损伤后CGRP的表达,减轻脑水肿,并能促进病理过程的恢复。
[Abstract]:Objective to investigate the protective effect of CGRP receptor antagonist CGRP8-37 on rats with traumatic brain injury. Methods 90 Wistar rats were randomly divided into control group (n = 6), trauma group (n = 42), CGRP8-37 group (n = 42). The changes of CGRP were observed in rats in the trauma and CGRP8-37 groups after 72 hours of injury and 24 h, 24 h and 48 h. Results 1Immunohistochemistry showed that the expression of CGRP positive units in brain tissue of TBI group increased from 0.5 h to 6 h after injury and reached the peak at 12 h (P0.05), then decreased gradually to the normal level at 72 h after injury. Compared with TBI group, the expression of CGRP positive units reached its peak at 2 h, and then returned to normal level at 48 h. At the corresponding time point, CGRP positive unit expression was lower than that in TBI group. 2PCR showed that CGRP mRNA expression in brain tissue of CGRP8-37 group was similar to that of TBI group, that is, CGRP mRNA expression in CGRP8-37 group was earlier than that in TBI group, from 12 h to 2 h, and CGRP mRNA expression was decreased at the corresponding time point. Conclusion CGRP receptor antagonist CGRP8-37 can reduce the expression of CGRP after traumatic brain injury, alleviate brain edema and promote the recovery of pathological process.
【作者单位】：山西医科大学;山西医科大学第一医院;
【基金】：国家自然基金资助项目(No.30600637)
【分类号】：R651.1

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