抗氧化剂对热打击人脐动脉血管平滑肌细胞收缩反应的影响
发布时间：2018-07-17 21:15
【摘要】：目的研究抗氧化剂对热打击过程中人脐动脉血管平滑肌细胞(HUASMCs)收缩反应的影响。方法将HUASMCs分为对照组、热打击组(HS组)和热打击前超氧化物歧化酶(SOD)预处理组(SOD+HS组),每组分别用低浓度NE(0.05mg/L)及常规浓度NE(1.0mg/L)刺激。各组细胞于41℃恒温水箱中分别培养0.5、1、1.5、2h后添加NE刺激,采用细胞表面积收缩比(%)反映细胞对NE的反应性。结果与对照组比较,无论是给予低浓度或常规浓度NE刺激,HS组细胞热打击1h时对于NE的反应性均明显增高,2h时对于NE的反应性则明显降低(P0.05或P0.01);SOD+HS组细胞对NE的反应性在热打击至2h后均出现明显下降(P0.05或P0.01)。对照组及HS组细胞对不同浓度NE刺激的反应性差异无统计学意义(P0.05),SOD+HS组细胞在热打击过程中对常规浓度NE的反应性明显高于对低剂量NE的反应性(P0.05或P0.01)。无论给予常规浓度或低浓度NE刺激,SOD+HS组细胞对NE的反应性均低于HS组(P0.05或P0.01)。结论热打击过程中,HUASMCs对NE的反应性呈时相性变化。抗氧化剂在中暑早期可能可以纠正HUASMCs对NE的过反应现象,但在热打击中后期,抗氧化剂并不能改善其反应性的下降。
[Abstract]:Aim to study the effects of antioxidants on the contractile response of human umbilical artery smooth muscle cells (HUASMCs) during thermal shock. Methods HUASMCs were divided into three groups: control group, heat shock group (HS group) and superoxide dismutase (SOD HS) pretreatment group (SOD HS group). Each group was stimulated with low concentration of NE (0.05 mg / L) and routine concentration of NE (1.0 mg / L). The cells in each group were cultured in a constant temperature water tank at 41 鈩，
本文编号：2130958
[Abstract]:Aim to study the effects of antioxidants on the contractile response of human umbilical artery smooth muscle cells (HUASMCs) during thermal shock. Methods HUASMCs were divided into three groups: control group, heat shock group (HS group) and superoxide dismutase (SOD HS) pretreatment group (SOD HS group). Each group was stimulated with low concentration of NE (0.05 mg / L) and routine concentration of NE (1.0 mg / L). The cells in each group were cultured in a constant temperature water tank at 41 鈩，
本文编号：2130958
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