雄激素对TBI模型大鼠Phosphacan和NG2蛋白聚糖表达与神经元轴突再生的影响研究

发布时间：2018-07-21 17:15

【摘要】：目的：制作SD大鼠TBI模型并采用雄激素干预治疗，了解雄激素对创伤性脑损伤(TBI)大鼠脑组织磷酸蛋白聚(phosphacan, PC)及NG2蛋白聚糖(NG2)表达影响与创伤性脑损伤后神经修复的关系,探讨其促神经再生的作用机制。方法 1、选择雄性Sprague-Dawley大鼠48只，体重为140g～200g。随机分为正常组、TBI模型组、雄激素干预组(于模型制成后即刻注射丙酸睾丸酮注射液25mg/kg)。 2、使用改进的FEENEY'S法制作SD大鼠TBI模型。 3、造模后24h、72h、5d、7d取脑组织制作石蜡切片，用免疫组化法用Anti-NG2/CSPG4、Anti-PTP zeta/Phosphacan标记。观察损伤区组织磷酸蛋白聚糖(phosphacan, PC)及NG2蛋白聚糖表（NG2）达动态变化，并采用灰度分析量化比较。 4、雄激素干预组造模后制电镜标本应用透射电镜对比观察损伤区及其周围的神经元超微结构变化、细胞损伤、凋亡、神经元轴突再生及胶质细胞增生情况。结果 1、雄激素干预组造模后24h、72h、5d、7d后脑组织损伤区及周围表达水平明显低于TBI组。 2、雄激素干预组24h、72h、5d和7d时细胞水肿,可见凋亡细胞，，TBI模型组细胞表面突起较少或接近与无;雄激素干预组的神经元轴突较TBI模型组显著增多 3、24h、72h、5d、7d损伤外围胶质细胞增生情况较TBI模型组明显降低结论雄激素可使PC和NG2表达抑制而促进神经元轴突再生,促进神经修复。
[Abstract]:Objective: to investigate the effect of androgen on the expression of phosphatase (PC) and NG2 proteoglycan (NG2) in the brain tissue of rats with traumatic brain injury (TBI) and to investigate the relationship between the effects of androgen on the nerve repair after traumatic brain injury (TBI). To explore the mechanism of promoting nerve regeneration. Methods 1. Forty-eight male Sprague-Dawley rats, weighing 140 g / 200 g, were selected. They were randomly divided into normal group and TBI model group. In the androgen intervention group (testosterone propionate injection 25mg/kg). 2. The SD rat model was made by using the modified FEENEYS method. Anti-NG2 / CSPG4 + Anti-PTP zeta / Phosphacan was labeled by immunohistochemical method. To observe the dynamic changes of phosphate proteoglycan (PC) and NG2 proteoglycan (NG2) in injured tissue. In androgen intervention group, the ultrastructural changes, cell injury and apoptosis of neurons in and around the injured area were observed by transmission electron microscope (TEM). Axon regeneration and glial cell proliferation of neurons. Results 1. The expression level of brain tissue injury area and surrounding area in androgen intervention group was significantly lower than that in TBI group at 24 h, 72 h and 5 d after establishment of the model, and the cell edema was found in androgen intervention group at 24 h, 72 h, 5 d and 7 d, respectively. It can be seen that the apoptotic cells in TBI model group have less or close to none of the surface processes. The neuronal axons in the androgen intervention group were significantly higher than those in the TBI model group. [WT5 "HZ] the proliferation of peripheral glial cells in the androgen intervention group was significantly lower than that in the TBI model group. Conclusion androgen can induce the expression of PC and NG2 in TBI model group. Inhibit and promote neuronal axon regeneration, Promote nerve repair.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R651.15

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