骨髓间质干细胞移植减轻犬急性脑梗死模型的缺血性脑损伤研究

发布时间：2018-08-06 15:44

【摘要】：目的观察骨髓间质干细胞(BMSCs)对犬急性缺血脑组织的保护作用,并探讨其可能的机制。方法将24只成年杂交犬随机分为治疗组及对照组,DSA引导下行自体血栓栓塞大脑中动脉闭塞缺血模型制作,并抽取骨髓提取BMSCs,传代并给予4’,6-二脒基-2-苯基吲哚(DAPI)标记;1周后开颅行多点脑内注射BMSCs移植;移植后1周行脑DWI序列扫描,计算梗死灶体积;4周后将犬处死,每组随机选择6只动物取脑标本行TTC染色测定梗死灶体积;另外6只动物进行HE染色、VG染色、TUNEL染色评价脑损伤情况;免疫荧光染色了解脑源性神经营养因子(BDNF)、碱性成纤维生长因子(b FGF)、胰岛素样生长因子1(IGF-1)和血管内皮生长因子(VEGF)的表达情况。结果治疗组梗死灶内广泛存在DAPI阳性细胞。治疗组的梗死灶体积明显小于对照组,P0.01。治疗组梗死灶范围、梗死灶内细胞坏死、胶质增生和胶质瘢痕均较对照组减轻。治疗组凋亡细胞明显少于对照组,P0.05。治疗组细胞因子BDNF、BFGF、IGF-1和VEGF表达阳性的细胞均显著多于对照组。结论脑梗死后给予BMSCs移植,BMSCs能存活并自行向梗死灶迁移,并发挥脑保护作用,其机制可能和分泌多种神经营养因子有关。
[Abstract]:Objective to investigate the protective effect of bone marrow mesenchymal stem cell (BMSCs) on acute ischemic brain tissue in dogs and its possible mechanism. Methods Twenty-four adult hybrid dogs were randomly divided into treatment group and control group. BMSCs were extracted from bone marrow, then subcultured and labeled with 4-diamidine-2-phenylindole-indole (DAPI) for 1 week, then BMSCs was injected into the brain after craniotomy, and brain DWI sequence scanning was performed 1 week after transplantation, and the dogs were killed after 4 weeks of infarct volume calculation. The infarct volume was determined by TTC staining in 6 animals in each group, and the brain injury was evaluated by HE staining with VG staining and Tunel staining. The expression of (BDNF), basic fibroblast growth factor (b FGF),) insulin-like growth factor 1 (IGF-1) and vascular endothelial growth factor (VEGF) were detected by immunofluorescence staining. Results DAPI positive cells were found in infarct focus of treatment group. The infarct volume in the treatment group was significantly smaller than that in the control group (P 0.01). The infarct area, cell necrosis, glial hyperplasia and glial scar in the treatment group were less than those in the control group. The apoptotic cells in the treatment group were significantly lower than those in the control group (P 0.05). The positive expression of BDNF BFGFF-1 and VEGF in the treatment group was significantly higher than that in the control group. Conclusion BMSCs can survive and migrate to the infarct area after cerebral infarction, and exert the protective effect of brain. The mechanism may be related to the secretion of many neurotrophic factors.
【作者单位】：江西省宜春市人民医院神经内科;
【基金】：江西省科技计划项目(20112BBG70085)
【分类号】：R-332;R743.33

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