犬肩袖损伤愈合模型的研究
发布时间：2018-08-13 11:07
【摘要】：目的比较研究不同肩袖损伤类型以及采用不同方法修复后的肩袖愈合差异,探讨能准确模拟临床肩袖损伤修复术后恢复进程的动物模型。方法取12只成年雄性比格犬,体质量10~15 kg,根据处理方法不同随机分为3组(n=4),分别制备急性肩袖损伤+Mason-Allen缝合修复(A组)、巨大肩袖损伤+Mason-Allen缝合修复(B组)及巨大肩袖损伤+Mason-Allen缝合并自体半腱肌扩张部修复(C组)动物模型;3组修复后均外固定架制动。术后观察各组动物一般情况;于术后6周取材大体观察冈下肌肌腱末端愈合情况,生物力学测试极限负荷,组织学观察肌腱细胞及纤维改变,比较肩袖修复程度。结果术后各组动物均存活至实验完成,切口均愈合良好,无感染发生。大体观察示,A组冈下肌肌腱末端瘢痕组织明显多于正常肌腱组织;B组冈下肌肌腱末端未见明显肌腱组织;C组冈下肌肌腱虽然部分覆盖瘢痕组织,但仍可观察到肌腱及其大致走向。A、B、C组极限负荷分别为(223.75±24.28)、(159.25±34.87)、(233.25±14.24)N,B组显著低于A、C组(P0.05),A、C组间比较差异无统计学意义(P0.05)。组织学观察示,A组肌腱纤维排列大致正常;B组肌腱纤维排列较紊乱,并且肌腱细胞明显少于A组;C组肌腱纤维排列整齐,且肌腱细胞多于B组。结论 Mason-Allen缝合联合自体半腱肌扩张部修复犬巨大肩袖损伤能获良好修复效果;制动模型可较好模拟临床肩袖损伤愈合过程,可作为相关研究的理想动物模型。
[Abstract]:Objective to compare the different types of rotator cuff injury and the difference of rotator cuff healing after repair with different methods, and to explore an animal model which can accurately simulate the recovery process of clinical rotator cuff injury repair. Methods 12 adult male Beagle dogs were selected. The patients were randomly divided into 3 groups according to different treatment methods: Mason-Allen suture repair for acute rotator cuff injury (group A), Mason-Allen suture repair for giant rotator cuff injury (group B) and Mason-Allen suture with autologous semitendinosus for giant rotator cuff injury. Muscle dilatation repair (group C) animal model group 3 after repair of external fixator brake. The general condition of each group was observed 6 weeks after operation, the healing of tendon end of inferior spinal muscle was observed, the limit load of biomechanics was measured, the changes of tendon cells and fibers were observed by histology, and the degree of rotator cuff repair was compared. Results all the animals survived to the end of the experiment, the incision healed well and no infection occurred. Gross observation showed that the scar tissue at the end of subspinous muscle in group A was significantly more than that in group B, and no obvious tendon tissue was found at the end of tendon in group C although the tendon in group C was partly covered with scar tissue. However, the ultimate load of tendon and its approximate direction in group C was (223.75 卤24.28), () 159.25 卤34.87), (233.25 卤14.24), (, respectively, which was significantly lower than that in group A (P 0.05). There was no significant difference between group A and C (P0.05). Histological observation showed that the arrangement of tendon fibers in group A was generally normal. The arrangement of tendon fibers in group B was disordered, and the number of tendon cells in group A was significantly less than that in group A and C, and the number of tendon cells in group B was more than that in group B. Conclusion Mason-Allen suture combined with autologous semitendinosus expansion can be used to repair the giant rotator cuff injury in dogs, and the immobilization model can be used to simulate the healing process of clinical rotator cuff injury, and it can be used as an ideal animal model for related research.
【作者单位】： 南方医科大学南京临床医学院附属南京军区南京总医院骨科;
【基金】：江苏省临床医学科技专项资助项目(BL2012002)~~
【分类号】：R686;R-332
本文编号：2180791
[Abstract]:Objective to compare the different types of rotator cuff injury and the difference of rotator cuff healing after repair with different methods, and to explore an animal model which can accurately simulate the recovery process of clinical rotator cuff injury repair. Methods 12 adult male Beagle dogs were selected. The patients were randomly divided into 3 groups according to different treatment methods: Mason-Allen suture repair for acute rotator cuff injury (group A), Mason-Allen suture repair for giant rotator cuff injury (group B) and Mason-Allen suture with autologous semitendinosus for giant rotator cuff injury. Muscle dilatation repair (group C) animal model group 3 after repair of external fixator brake. The general condition of each group was observed 6 weeks after operation, the healing of tendon end of inferior spinal muscle was observed, the limit load of biomechanics was measured, the changes of tendon cells and fibers were observed by histology, and the degree of rotator cuff repair was compared. Results all the animals survived to the end of the experiment, the incision healed well and no infection occurred. Gross observation showed that the scar tissue at the end of subspinous muscle in group A was significantly more than that in group B, and no obvious tendon tissue was found at the end of tendon in group C although the tendon in group C was partly covered with scar tissue. However, the ultimate load of tendon and its approximate direction in group C was (223.75 卤24.28), () 159.25 卤34.87), (233.25 卤14.24), (, respectively, which was significantly lower than that in group A (P 0.05). There was no significant difference between group A and C (P0.05). Histological observation showed that the arrangement of tendon fibers in group A was generally normal. The arrangement of tendon fibers in group B was disordered, and the number of tendon cells in group A was significantly less than that in group A and C, and the number of tendon cells in group B was more than that in group B. Conclusion Mason-Allen suture combined with autologous semitendinosus expansion can be used to repair the giant rotator cuff injury in dogs, and the immobilization model can be used to simulate the healing process of clinical rotator cuff injury, and it can be used as an ideal animal model for related research.
【作者单位】： 南方医科大学南京临床医学院附属南京军区南京总医院骨科;
【基金】：江苏省临床医学科技专项资助项目(BL2012002)~~
【分类号】：R686;R-332
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1 李奉龙;姜春岩;鲁谊;黎广平;卢耀甲;;兔肩袖损伤模型的建立及初步组织学研究[J];中国组织工程研究;2012年20期
，本文编号：2180791
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