急性脑梗死患者外周血单个核细胞中组蛋白H3乙酰化水平的变化

发布时间：2018-09-10 06:08

【摘要】：目的探讨急性脑梗死患者外周血单个核细胞(PBMC)中组蛋白H3乙酰化水平变化与急性脑梗死的临床关系。方法选取2015年1~12月该院神经内科收治的发病后不同时间段的急性脑梗死患者206例为脑梗死组;选取同期在该院脑卒中筛查门诊就诊的60名健康志愿者为对照组。密度梯度法离心分离PBMC,提取全组蛋白,Western印迹检测组蛋白H3乙酰化水平;根据患者就诊时的症状与体征,进行牛津郡社区脑卒中项目(OCSP)分型,并采用神经功能缺损程度评估(NIHSS)量表评估患者的神经功能缺损程度。结果急性脑梗死后1 d开始,持续到7 d,PBMC组蛋白H3乙酰化水平均明显低于对照组(P0.05),其中梗死后第3天蛋白H3值最低。不同OCSP分型患者组蛋白H3乙酰化水平均明显低于对照组(P0.05);其中完全前循环脑梗死(TACI)组蛋白H3乙酰化水平降低幅度最大,明显低于后循环脑梗死组和部分前循环脑梗死组(P0.05)。多元直线回归分析显示,PBMC组蛋白H3乙酰化水平是入院急性脑梗死患者NIHSS评分的重要影响因素,两者呈明显直线负相关(β=-0.283,P=0.001)。结论急性脑梗死患者PBMC中组蛋白H3乙酰化水平低于健康人群,且以TACI型和神经功能缺损严重患者的PBMC中组蛋白H3乙酰化水平下降最为明显。
[Abstract]:Objective to investigate the clinical relationship between histone H 3 acetylation in peripheral blood mononuclear cells (PBMC) and acute cerebral infarction (ACI) in patients with acute cerebral infarction (ACI). Methods 206 patients with acute cerebral infarction in different period after onset were selected as cerebral infarction group from January to December 2015, and 60 healthy volunteers were selected as control group. The acetylation level of histone H3 was detected by Western blotting of PBMC, extracted by density gradient centrifugation. According to the symptoms and signs of the patients, the (OCSP) typing of the stroke project in Oxfordshire community was carried out. The degree of neurological impairment was evaluated by (NIHSS) scale. Results the level of histone H3 acetylation in PBMC was significantly lower than that in the control group (P0.05) from day 1 to day 7 after acute cerebral infarction. The lowest level of H3 was observed on day 3 after acute cerebral infarction. The level of histone H3 acetylation in patients with different OCSP classification was significantly lower than that in the control group (P0.05), and the level of H3 acetylation of (TACI) in patients with complete anterior circulation infarction was the largest, which was significantly lower than that in the posterior circulation cerebral infarction group and part of anterior circulation cerebral infarction group (P0.05). Multiple linear regression analysis showed that histone H _ 3 acetylation was an important factor in NIHSS score in patients with acute cerebral infarction (尾 -0.283). Conclusion the level of histone H _ 3 acetylation in PBMC in patients with acute cerebral infarction is lower than that in healthy people, and the level of histone H _ 3 acetylation in PBMC of patients with TACI type and severe neurological impairment is the most obvious.
【作者单位】：福州神经精神病防治院神经科;
【基金】：福建省自然科学基金资助项目(No:2014D013)
【分类号】：R743.33

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