谷氨酰胺对脓毒症大鼠心肌的保护作用及其机制的研究

发布时间：2018-11-05 11:04

【摘要】：目的研究谷氨酰胺对脓毒症大鼠心肌的保护作用，并探讨其作用机制。方法选取SPF级健康成年大鼠42只随机分成三组：假手术组(CON)6只，手术组（CLP）18只，谷氨酰胺组（GLN）18只，采用盲肠结扎穿孔法(cecal ligationand puncture,CLP)制备脓毒症模型。其中手术组和谷氨酰胺组大鼠于6h、12h、24h三个时相点各取6只大鼠心脏标本，每个识相点包含6只手术组大鼠和6只谷氨酰胺组大鼠。TUNEL法检测各时相点大鼠心肌凋亡指数，PCR法检测各时相点大鼠心肌Bcl-2及p53mRNA表达。术后6h每组收集血清，取心肌，检测血清中LDH(乳酸脱氢酶)、CK（磷酸肌酸激酶）、TNI(肌钙蛋白I)水平。结果手术组术后6h、12h、24h的心肌细胞Bcl-2表达较假手术组明显下调，而p53mRNA的表达较假手术组明显上调（P＜0.05）；谷氨酰胺组术后6h、12h、24h的心肌细胞Bcl-2mRNA表达较手术组明显上调，而p53mRNA的表达较手术组明显下调（P＜0.05）。手术组各时相点心肌细胞凋亡率明显高于假手术组，而谷氨酰胺组心肌细胞凋亡率明显低于手术组（P＜0.05）。CLP和GLN组血清中LDH、CK、TNI水平均显著高于CON组(P值分别0.05、0.01)；GLN组血清LDH、CK、TNI水平显著低于CLP组(P 0.05)，两组间均存在显著性差异(P值分别0.01，0.05)。结论1.谷氨酰胺可以明显降低脓毒症大鼠血清中LDH、CK、TNI水平，，对脓毒症大鼠心肌有显著的保护作用。2.脓毒症大鼠心肌凋亡明显增高，谷氨酰胺可以通过影响Bcl-2及p53的表达来显著抑制脓毒症大鼠心肌细胞的凋亡。
[Abstract]:Objective to study the protective effect of glutamine on myocardium of septic rats and its mechanism. Methods Forty-two healthy adult SPF rats were randomly divided into three groups: sham operation group (CON) group, n = 6), operation group (CLP) group, n = 18) and glutamine group (GLN) group, n = 18). Sepsis model was established by cecal ligation and perforation (cecal ligationand puncture,CLP). Among them, 6 heart samples were taken from each group at 6 h and 12 h / 24 h after operation and glutamine group. Each phase point included 6 rats in the operation group and 6 rats in the glutamine group. The myocardial apoptosis index was measured by TUNEL method. The expression of Bcl-2 and p53mRNA in myocardium of rats at different time points was detected by PCR method. Serum samples were collected from each group 6 hours after operation, and the serum LDH (lactate dehydrogenase), CK (creatine kinase), TNI (troponin I) was measured. Results the expression of Bcl-2 in cardiomyocytes in the operation group was significantly lower than that in the sham operation group at 6 hours and 12 hours after operation, while the expression of p53mRNA in the operation group was significantly higher than that in the sham operation group (P < 0. 05). The expression of Bcl-2mRNA in cardiac myocytes in the glutamine group was significantly higher than that in the operation group at 6 h and 12 h and 24 h after operation, while the expression of p53mRNA was significantly lower than that in the operation group (P < 0. 05). The apoptosis rate of cardiac myocytes in the operation group was significantly higher than that in the sham operation group, while the apoptosis rate in the glutamine group was significantly lower than that in the operation group (P < 0. 05). CLP and GLN group). The level of TNI was significantly higher in CON group than that in CON group (P < 0.05). The level of serum LDH,CK,TNI in GLN group was significantly lower than that in CLP group (P 0.05), and there was significant difference between the two groups (P < 0.01). Conclusion 1. Glutamine can significantly reduce the level of LDH,CK,TNI in serum of septic rats, and has a significant protective effect on myocardium of septic rats. 2. Myocardial apoptosis was significantly increased in septic rats. Glutamine could significantly inhibit the apoptosis of myocardial cells in septic rats by affecting the expression of Bcl-2 and p53.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7

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