颅脑损伤促进的坐骨神经再生

发布时间：2018-11-26 10:12

【摘要】：背景:研究表明大鼠颅脑损伤对坐骨神经损伤的修复有促进作用,然而其具体机制尚不明确。目的:于形态学和组织学进一步探索颅脑损伤对坐骨神经损伤修复的作用机制。方法:选用SPF级健康雄性SD大鼠60只,随机分为2组,颅脑损伤合并坐骨神经损伤模型为实验组,单纯坐骨神经损伤模型为对照组。采用经典Feeney法颅脑损伤模型和Sunderland V型坐骨神经损伤模型。于造模后8,12周检测大鼠坐骨神经指数;行坐骨神经吻合处神经组织Masson染色、NF200免疫荧光化学染色观察神经再生情况;采用透射电镜观察再生轴突数。结果与结论:造模后8,12周,与对照组相比,实验组大鼠步态、坐骨神经指数恢复更好。实验组大鼠Masson染色显示神经外膜胶原纤维增生较少、轴突排列整齐。NF200免疫荧光染色显示实验组再生神经密度高、排列规律。透射电镜显示实验组再生轴突排列整齐、胶原瘢痕增生较少、髓鞘板层分布整齐、规则。结果提示大鼠颅脑损伤可能通过减少神经断端胶原瘢痕形成促进周围神经损伤的修复。
[Abstract]:Background: the research shows that craniocerebral injury can promote the repair of sciatic nerve injury in rats, but its mechanism is not clear. Objective: to explore the mechanism of craniocerebral injury on sciatic nerve injury by morphology and histology. Methods: sixty SPF grade healthy male SD rats were randomly divided into 2 groups. The model of craniocerebral injury combined with sciatic nerve injury was the experimental group, and the simple sciatic nerve injury model was the control group. The classical Feeney model and the Sunderland V model of sciatic nerve injury were used. The sciatic nerve index was measured at 8 and 12 weeks after modeling, the nerve tissue Masson staining and NF200 immunofluorescence staining were used to observe the regeneration of sciatic nerve, and the number of regenerated axons was observed by transmission electron microscope. Results and conclusion: compared with the control group, the gait and sciatic nerve index of the experimental group were better than that of the control group at 812 weeks. Masson staining showed that the proliferation of collagenous fibers in the epineurium was less and the axons were arranged neatly. NF200 immunofluorescence staining showed that the regenerated nerve in the experimental group had a high density and regular arrangement. Transmission electron microscopy showed that the regenerated axons in the experimental group were arranged neatly, the collagen scar proliferation was less, and the myelin sheathing lamina was distributed neatly and regularly. The results suggest that craniocerebral injury may promote the repair of peripheral nerve injury by reducing collagen scar formation.
【作者单位】：承德医学院附属医院手足外科;秦皇岛市第一医院手足外科;承德医学院研究生学院;承德医学院附属医院神经外科;
【基金】：河北省卫生厅指令性课题(20130027) 河北省科技厅指令性课题(142777105D) 河北省承德市科技局课题(20123128)~~
【分类号】：R651.15;R745

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