血浆微小RNA-208a、210、506与急性心肌梗死的相关性

发布时间：2018-12-18 01:09

【摘要】：目的探讨血浆微小RNA-208a、210、506在急性心肌梗死(AMI)早期诊断中的意义及其与AMI严重程度的相关性,并进一步探讨其在延边地区朝鲜族与汉族AMI患者间的表达差异。方法将研究对象分为正常对照组和AMI组,AMI组又分为急诊手术亚组、择期手术亚组和Gensini评分四个亚组。采用实时荧光定量聚合酶链反应检测血浆miR-208a、210、506相对表达量。结果 AMI组miR-208a、miR-506相对表达量较对照组升高(P0.01,P0.05),miR-210较对照组降低(P0.01);急诊手术组miR-208a较择期手术组升高(P0.05)。三种miRNAs在朝鲜族与汉族间无明显差异。MiR-208a与年龄、肌酸磷酸激酶同工酶(CK-MB)、肌钙蛋白Ⅰ(c Tn I)、pro-BNP呈显著正相关,miR-210与年龄、pro-BNP呈负相关,miR-506与低密度脂蛋白胆固醇(LDL-C)、CK-MB、c Tn I、pro-BNP呈正相关。Gensini评分与miR-208a、miR-506呈显著正相关,与miR-210呈显著负相关。结论 MiR-208、210、506可作为AMI的诊断标记物,其中miR-208可能为早期诊断AMI的生物标志物。miR-208a具有评价冠状动脉粥样硬化严重程度并进行危险分层的潜力。AMI患者血浆miR-208a、miR-210、miR-506的表达水平无地区、民族差异。
[Abstract]:Objective to investigate the significance of plasma microRNA-208a,210506 in the early diagnosis of acute myocardial infarction (AMI) (AMI) and its correlation with the severity of AMI, and to explore the difference of its expression in AMI patients of Korean nationality and Han nationality in Yanbian area. Methods the subjects were divided into normal control group and AMI group, and AMI group was divided into emergency operation subgroup, selective operation subgroup and Gensini score subgroup. The relative expression of miR-208a,210506 in plasma was detected by real-time fluorescence quantitative polymerase chain reaction. Results the relative expression of miR-208a,miR-506 in AMI group was higher than that in control group (P0.05), miR-210 was lower than that in control group (P0.01), and miR-208a in emergency operation group was higher than that in elective operation group (P0.05). MiR-208a was positively correlated with age, creatine phosphokinase isoenzyme (CK-MB), troponin I (c Tn I), pro-BNP, and miR-210 was negatively correlated with age and pro-BNP. MiR-506 was positively correlated with low density lipoprotein cholesterol (LDL-C) and CK-MB,c Tn pro-BNP, and Gensini score was positively correlated with miR-208a,miR-506 and negatively correlated with miR-210. Conclusion MiR-208210506 can be used as a diagnostic marker for AMI, and miR-208 may be a biomarker for early diagnosis of AMI. MiR-208a has the potential to evaluate the severity of coronary atherosclerosis and risk stratification. The plasma miR-208a, in AMI patients is likely to be a biomarker for early diagnosis of AMI. The expression level of miR-210,miR-506 had no regional and ethnic differences.
【作者单位】：延边大学附属医院;
【基金】：吉林省科技发展计划项目(No.20140414065GH)
【分类号】：R542.22

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