鲍曼不动杆菌脓毒症大鼠体内TCTP表达情况及血必净对其表达的影响
发布时间：2018-12-20 10:30
【摘要】：目的：通过研究鲍曼不动杆菌脓毒症大鼠中性粒细胞和肝脏组织中翻译控制肿瘤蛋白(Thetranslationallycontrolledtumorprotein，TCTP)在不同时间点的表达情况，并以血必净进行干预治疗，检测炎症因子IL-6、IL-10及肝脏组织病理变化，探讨TCTP与鲍曼不动杆菌脓毒症之间的关系，及血必净对其表达的影响。 方法：1、42只清洁级成年雄性wistar大鼠随机分成7组：正常对照组（N），鲍曼不动杆菌脓毒症6h、12h、24h组(B6h、B12h、B24h），血必净治疗6h、12h、24h组(X6h、X12h、X24h）。其中鲍曼不动杆菌脓毒症各组及血必净治疗各组大鼠分别腹腔注射鲍曼不动杆菌悬液，正常对照组大鼠腹腔注射等量生理盐水；血必净治疗各组大鼠尾静脉注射血必净，余各组大鼠尾静脉注射等量生理盐水。2、正常组、不同时间点鲍曼不动杆菌脓毒症组及血必净治疗组大鼠颈动脉采血后取肝脏，用westernblot检测中性粒细胞内TCTP的表达变化，ELISA检测血浆中炎症因子IL-6、IL-10的变化，，用westernblot及免疫组化检测肝脏组织中TCTP表达情况，HE染色法观察肝脏组织病理改变。 结果：1、与正常对照组相比，鲍曼不动杆菌脓毒症各组中性粒细胞和肝脏组织中TCTP表达量增高，且于12小时达高峰(P0.05)；与鲍曼不动杆菌脓毒症各组相比，血必净治疗各组中性粒细胞和肝脏组织中TCTP表达量分别较对应组降低(P0.05)；2、与正常对照组相比，鲍曼不动杆菌脓毒症各组血浆中IL-6、IL-10含量增加，且于12小时达高峰(P0.05)；与鲍曼不动杆菌脓毒症各组相比，血必净治疗各组血浆中IL-6表达量分别较对应组降低(P0.05),而IL-10升高(P0.05)；3、正常对照组大鼠的肝脏组织中，仅可见散在的TCTP阳性细胞且颜色较浅，而鲍曼不动杆菌脓毒症各组肝脏组织中可见大量的TCTP阳性细胞存在且颜色较深，血必净治疗组肝脏组织情况介于二者之间(P0.05)；4、病理切片HE染色可见鲍曼不动杆菌脓毒症各组肝脏组织炎症反应、细胞损伤较明显，血必净治疗各组则较其减轻，正常对照组未见明显异常。 结论：1、TCTP在鲍曼不动杆菌脓毒症各组大鼠中性粒细胞及肝脏组织中的表达增加，且于12小时达高峰，TCTP可能在鲍曼不动杆菌脓毒症发生、发展过程中具有重要意义；2、血必净注射液可减轻鲍曼不动杆菌脓毒症大鼠的炎症反应，减少血浆中促炎性细胞因子IL-6的释放，增加抑炎性细胞因子IL-10的释放，并降低中性粒细胞及肝脏中TCTP的表达含量；3、血必净注射液可能通过减少TCTP的表达，继而影响促炎性细胞因子IL-6的释放而发挥抗炎作用。
[Abstract]:Objective: to study the expression of translation-controlled tumor protein (Thetranslationallycontrolledtumorprotein,TCTP) in neutrophils and liver tissues of Acinetobacter baumannii sepsis rats at different time points, and to detect the inflammatory factor IL-6, after intervention with Xiebijing. To investigate the relationship between TCTP and Acinetobacter baumannii sepsis and the effect of Xue Bijing on the expression of IL-10 and liver histopathology. Methods: 1Forty-two clean grade adult male wistar rats were randomly divided into 7 groups: the normal control group, (N), the control group was treated with acinetobacter baumannii sepsis for 6 h and 12 h, and the control group was treated with B6 h B12 h and B24 h, X6 h, X 12 h, X 24 h, and X6 h, X 12 h, X 24 h, respectively. Acinetobacter baumannii sepsis group and Xuebijing treatment group rats were intraperitoneally injected with Acinetobacter baumannii suspension and normal control group rats intraperitoneal injection of the same amount of normal saline; Xuebijing treated rats in each group were injected with Xuebijing via tail vein, and rats in other groups were injected with the same amount of normal saline. 2. Normal group, Acinetobacter baumannii sepsis group at different time points and Xuebijing treatment group, the liver was taken from carotid artery of rats. The expression of TCTP in neutrophil was detected by westernblot, the expression of inflammatory factor IL-6,IL-10 in plasma was detected by ELISA, the expression of TCTP in liver was detected by westernblot and immunohistochemistry, and the pathological changes of liver were observed by HE staining. Results: 1. Compared with the normal control group, the expression of TCTP in neutrophils and liver tissues of Acinetobacter baumannii sepsis groups increased, and reached the peak at 12 hours (P0.05). Compared with each group of Acinetobacter baumannii sepsis, the expression of TCTP in neutrophils and liver tissue of Xuebijing group was lower than that of corresponding group (P0.05). 2. Compared with the normal control group, the plasma IL-6,IL-10 content of Acinetobacter baumannii sepsis group increased and reached its peak at 12 hours (P0.05). Compared with each group of Acinetobacter baumannii sepsis, the expression of IL-6 in plasma of Xuebijing group was lower than that of corresponding group (P0.05), but IL-10 was increased (P0.05). 3. In normal control group, only scattered TCTP positive cells were found in the liver tissue and the color was lighter, while in Acinetobacter baumannii sepsis group, a large number of TCTP positive cells were found in the liver tissue and the color was darker than that in the control group. The liver tissue of Xuebijing treatment group was between the two groups (P0.05). 4. HE staining of pathological sections showed that the inflammatory reaction of liver tissue was obvious in each group of Acinetobacter baumannii sepsis, the cell damage was more obvious, the treatment group of Xue Bijing was lighter than that of the control group, and no obvious abnormality was found in the normal control group. Conclusion: 1 the expression of TCTP in the neutrophils and liver tissues of rats with Acinetobacter baumannii sepsis increased and peaked at 12 hours. TCTP may play an important role in the occurrence and development of Acinetobacter baumannii sepsis. 2. Xuebijing injection can reduce the inflammatory response of Acinetobacter baumannii sepsis rats, reduce the release of pro-inflammatory cytokine IL-6 in plasma, and increase the release of anti-inflammatory cytokine IL-10. The expression of TCTP in neutrophils and liver was also decreased. 3. Xuebijing injection may play an anti-inflammatory effect by reducing the expression of TCTP and then affecting the release of pro-inflammatory cytokine IL-6.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7
本文编号：2387868
[Abstract]:Objective: to study the expression of translation-controlled tumor protein (Thetranslationallycontrolledtumorprotein,TCTP) in neutrophils and liver tissues of Acinetobacter baumannii sepsis rats at different time points, and to detect the inflammatory factor IL-6, after intervention with Xiebijing. To investigate the relationship between TCTP and Acinetobacter baumannii sepsis and the effect of Xue Bijing on the expression of IL-10 and liver histopathology. Methods: 1Forty-two clean grade adult male wistar rats were randomly divided into 7 groups: the normal control group, (N), the control group was treated with acinetobacter baumannii sepsis for 6 h and 12 h, and the control group was treated with B6 h B12 h and B24 h, X6 h, X 12 h, X 24 h, and X6 h, X 12 h, X 24 h, respectively. Acinetobacter baumannii sepsis group and Xuebijing treatment group rats were intraperitoneally injected with Acinetobacter baumannii suspension and normal control group rats intraperitoneal injection of the same amount of normal saline; Xuebijing treated rats in each group were injected with Xuebijing via tail vein, and rats in other groups were injected with the same amount of normal saline. 2. Normal group, Acinetobacter baumannii sepsis group at different time points and Xuebijing treatment group, the liver was taken from carotid artery of rats. The expression of TCTP in neutrophil was detected by westernblot, the expression of inflammatory factor IL-6,IL-10 in plasma was detected by ELISA, the expression of TCTP in liver was detected by westernblot and immunohistochemistry, and the pathological changes of liver were observed by HE staining. Results: 1. Compared with the normal control group, the expression of TCTP in neutrophils and liver tissues of Acinetobacter baumannii sepsis groups increased, and reached the peak at 12 hours (P0.05). Compared with each group of Acinetobacter baumannii sepsis, the expression of TCTP in neutrophils and liver tissue of Xuebijing group was lower than that of corresponding group (P0.05). 2. Compared with the normal control group, the plasma IL-6,IL-10 content of Acinetobacter baumannii sepsis group increased and reached its peak at 12 hours (P0.05). Compared with each group of Acinetobacter baumannii sepsis, the expression of IL-6 in plasma of Xuebijing group was lower than that of corresponding group (P0.05), but IL-10 was increased (P0.05). 3. In normal control group, only scattered TCTP positive cells were found in the liver tissue and the color was lighter, while in Acinetobacter baumannii sepsis group, a large number of TCTP positive cells were found in the liver tissue and the color was darker than that in the control group. The liver tissue of Xuebijing treatment group was between the two groups (P0.05). 4. HE staining of pathological sections showed that the inflammatory reaction of liver tissue was obvious in each group of Acinetobacter baumannii sepsis, the cell damage was more obvious, the treatment group of Xue Bijing was lighter than that of the control group, and no obvious abnormality was found in the normal control group. Conclusion: 1 the expression of TCTP in the neutrophils and liver tissues of rats with Acinetobacter baumannii sepsis increased and peaked at 12 hours. TCTP may play an important role in the occurrence and development of Acinetobacter baumannii sepsis. 2. Xuebijing injection can reduce the inflammatory response of Acinetobacter baumannii sepsis rats, reduce the release of pro-inflammatory cytokine IL-6 in plasma, and increase the release of anti-inflammatory cytokine IL-10. The expression of TCTP in neutrophils and liver was also decreased. 3. Xuebijing injection may play an anti-inflammatory effect by reducing the expression of TCTP and then affecting the release of pro-inflammatory cytokine IL-6.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R459.7
【参考文献】
相关期刊论文 前10条
1 张淑文;苏强;王超;阴峧宏;王红;王宝恩;;脓毒性休克患者的病死率和危险因素研究[J];创伤外科杂志;2007年01期
2 李宏伟;刘进辉;白晓龙;刘多;;血必净注射液在烧伤脓毒血症治疗中的应用[J];长春师范学院学报;2012年12期
3 朱任媛;张小江;杨启文;王瑶;王贺;陈雨;佟大伟;吕媛;;卫生部全国细菌耐药监测网2011年ICU来源细菌耐药监测[J];中国临床药理学杂志;2012年12期
4 郦珊珊;黄培志;童朝阳;姚晨玲;;不同程度脓毒症患者外周血中性粒细胞凋亡率改变及其机制的探讨[J];中国临床医学;2008年05期
5 张玉楚;陈童恩;刘鹏;杨群;陈培服;;连续性血液净化在救治脓毒血症患者中的应用[J];现代实用医学;2009年02期
6 王金惠,善亚君,从玉文,吴岚军,苑晓玲,赵振虎,王升启,陈家佩;人体肝癌细胞急性低氧及低氧习服差异表达基因分析[J];生理学报;2003年03期
7 何国鑫;胡慧敏;罗文朝;卢中秋;;血必净注射液对急性肺损伤大鼠氧自由基变化的影响[J];实用医学杂志;2009年12期
8 明自强;俞林明;吕银祥;王黎卫;;血必净对脓毒症患者细胞因子的影响[J];现代中西医结合杂志;2008年27期
9 高青芳;马小美;刘虹;;血必净对肠源性内毒素血症引起的急性肝损伤的保护作用[J];中国药物与临床;2010年07期
10 胡泽华;王琳琳;;内毒素血症肝损伤机制的研究进展[J];医学综述;2009年07期
相关硕士学位论文 前1条
1 马世堂;血必净防治脓毒症作用机制的研究[D];中国协和医科大学;2010年
本文编号：2387868
本文链接：https://www.wllwen.com/yixuelunwen/jjyx/2387868.html
最近更新
教材专著
