大鼠肺缺血再灌注组织中microRNA-451与蛋白激酶B的表达变化

发布时间：2019-02-14 10:11

【摘要】：目的：探讨不同时相大鼠肺缺血再灌注模型中microRNA-451及蛋白激酶B(p-AKT)的表达情况及二者之间的相关性。 方法：将Wistar大鼠随机分为五组,分别为对照组、缺血1h组、缺血1h后再灌注组1h、再灌注3h及再灌注6h组,每组10只,参照EPPINGER法建立大鼠肺缺血模型(分别于缺血1h、缺血1h后再灌注组1h、再灌注3h及再灌注6h取材),采用实时荧光定量PCR(RT-qPCR)检测microRNA-451及蛋白免疫印迹法(Western blot)检测p-AKT蛋白的表达情况。 结果:RT-qPCR检测结果显示microRNA-451在肺缺血再灌注过程中呈现先升高后降低的趋势,其中再灌注3h时达到最高点,差异有统计学意义(F=17244.32p0.05);Western blot检测法显示p-AKT蛋白呈先下降后升高的趋势,对照组及再灌注6h时呈高表达,再灌注3h时表达量最低,差异有显著性(F=136.02p0.05)。 结论：肺缺血再灌注过程中,microR-451与p-AKT的表达变化呈现一定的相关性,结合相关文献,可推测microR-451可能通过调节AKT蛋白通路促进细胞凋亡。
[Abstract]:Aim: to investigate the expression of microRNA-451 and protein kinase B (p-AKT) in lung ischemia-reperfusion model of rats and the correlation between them. Methods: Wistar rats were randomly divided into five groups: control group, 1 hour ischemia group, 1 h reperfusion group, 3 h reperfusion group and 6 h reperfusion group, 10 rats in each group. Lung ischemia models were established by EPPINGER method. The expression of p-AKT protein was detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot (Western blot). Results: the results of RT-qPCR showed that microRNA-451 increased at first and then decreased during lung ischemia-reperfusion, and reached the highest point at 3 h after reperfusion, and the difference was statistically significant (F=17244.32p0.05). Western blot assay showed that the expression of p-AKT protein decreased first and then increased. The expression of p-AKT protein in the control group was higher than that in the control group at 6 h and the lowest at 3 h after reperfusion. The difference was significant (F=136.02p0.05). Conclusion: the expression of microR-451 and p-AKT is correlated during lung ischemia-reperfusion. It can be speculated that microR-451 can promote apoptosis by regulating AKT protein pathway.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R655.3

【参考文献】

相关期刊论文 前1条

1 周晓东,于皆平,于红刚,罗和生,吕农华,朱萱;蛋白激酶B在胃癌中的表达及其生物学意义[J];中华消化杂志;2005年07期

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