川芎嗪联合运动训练对急性脑梗死模型大鼠星形胶质细胞与突触和运动功能的影响

发布时间：2019-02-16 13:13

【摘要】：目的探讨川芎嗪联合运动训练对急性脑梗死大鼠星形胶质细胞、突触、运功功能的影响。方法将120只成功造模急性脑梗死大鼠随机分为空白组、运动训练组(运动组)、川芎嗪注射组(川芎嗪组)和川芎嗪联合运动训练组(联合组)。于术后1、3、6 w采用显微电镜观察法计数四组大鼠病灶周围星形胶质细胞和突触数量;采用免疫组化法测定四组大鼠病灶周围胶质纤维酸性蛋白(GFAP)、生长相关蛋白(GAP)-43和突触素的含量;采用横木行走试验评价四组大鼠运动功能的恢复状况。结果运动组、川芎嗪组和联合组3个时间点的星形胶质细胞和突触数量均逐步增加,且均多于同时间点的空白组,但联合组的数量明显多于另外两组(P0.05);运动组、川芎嗪组和联合组3个时间点GFAP、GAP-43和突触素的含量均高于同时间点的空白组,但联合组的含量明显多于另外两组(P0.05);运动组、川芎嗪组和联合组3个时间点的运动功能评价得分均逐步上升,且均多于同时间点的空白组,但联合组的得分明显高于另外两组(P0.05)。结论采用川芎嗪联合运动训练对急性脑梗死大鼠进行治疗,可以帮助促进大鼠星形胶质细胞和突触的增加和运动功能的恢复,且其效果好于进行单纯运动训练和川芎嗪注射治疗的大鼠。
[Abstract]:Objective to investigate the effects of ligustrazine combined with exercise training on astrocyte synapse and motor function in rats with acute cerebral infarction. Methods 120 rats with acute cerebral infarction were randomly divided into control group, exercise training group, ligustrazine injection group and ligustrazine combined exercise training group. The number of astrocytes and synapses around the focus of the four groups were counted by microelectronic microscope at 1: 3 and 6 weeks postoperatively. The contents of glial fibrillary acidic protein (GFAP),) growth-associated protein (GAP) 43) and synaptophysin (synaptophysin) in peripheral glial fibrillary acidic protein (GFAP),) were determined by immunohistochemical method, and the recovery of motor function in four groups was evaluated by transverse walk test. Results the number of astrocytes and synapses in exercise group, ligustrazine group and combined group increased gradually, and were more than those in blank group at the same time point, but the number of combined group was significantly more than that of the other two groups (P0.05). The levels of GFAP,GAP-43 and synaptophysin in exercise group, ligustrazine group and combined group were higher than those in blank group at the same time point, but the content of synaptophysin in combined group was significantly higher than that in other two groups (P0.05). The motor function evaluation scores of exercise group, ligustrazine group and combined group increased gradually, and were higher than those of blank group at the same time point, but the score of the combined group was significantly higher than that of the other two groups (P0.05). Conclusion Tetramethylpyrazine combined with exercise training can promote the increase of astrocytes and synapses and the recovery of motor function in rats with acute cerebral infarction. The effect was better than that of rats treated with simple exercise training and ligustrazine injection.
【作者单位】：黄河科技学院;
【分类号】：R743.3;R-332

【相似文献】