大黄对严重烧伤大鼠肠源性真菌感染防治作用的研究
发布时间：2019-02-28 12:22
【摘要】：目的：探讨大黄对严重烧伤大鼠肠源性真菌感染的防治作用。 方法：选取健康Wistar大鼠72只,随机分成A、B、C三组,并分别以7×108个/ml浓度的白色念珠菌菌液灌胃,造成肠源性真菌感染模型。其中,A组为健康鼠菌液灌胃,B组为烫伤鼠菌液灌胃,C组为烫伤鼠菌液灌胃并在5分钟后以50%大黄液按5ml/kg灌胃。之后分不同时段(6h,12h,24h,48h)将大鼠处死,并立即抽取大鼠心脏静脉血液做肝功能和肾功能的检测；取各组大鼠肺脏组织和肠粘膜组织,通过ELISA法分别检测髓过氧化物酶和热休克蛋白-70；采用实时荧光定量PCR法检测各组大鼠肺脏、肝脏、肠系膜淋巴结和肾脏组织中白色念珠菌含量的CT值。 结果：①肝功能静脉血检测结果B组ALT和AST在同一时间段都较A组明显升高,P0.001；C组较B组在同一时间段下降明显,P值分别小于0.01和0.05,有统计学意义。②肾功能静脉血检测结果A组各时间段BUN和Cr都处于正常水平；B组与A组比较,6小时和12小时时间段无显示明显升高,P0.05,无统计学意义,24小时和48小时时间段升高明显,P0.001,有显著性差异；C组与B组比较,BUN和Cr在6小时和12小时时间段无显示明显升高,P0.05,无统计学意义,24小时和48小时时间段升高明显,P值分别小于0.01和0.05,有显著性差异。③肺部MPO结果B组较A组在同一时间段增高很多,P0.001,有统计学意义；而C组较B组在同一时间段下降明显,P0.001。④肠道HSP-70结果B组较A组明显升高,P0.001；而C组较B组也有不同程度升高,6小时段和24小时段P值均小于0.05,12小时段和48小时段P值均小于0.001。⑤荧光定量PCR法检测结果肺脏组织A组48小时开始检测到含有白色念珠菌；B组从12小时段开始检测到含有白色念珠菌,且在24小时和48小时段CT值都较前一时间段下降,说明菌含量在不断上升,与A组同一时间段比较,P0.01,有显著性差异；C组6小时和12小时时间段CT值大于35,没有检测到白色念珠菌,与B组比较,12小时段P0.01,24小时和48小时段P0.05,有统计学意义；肝脏组织中真菌检测到的时间段在A组为12小时,B组为6小时,B组同一时间段与A组比较,CT值都下降明显,P0.01,使用大黄后,CT值又有明显升高,P0.05；肠系膜淋巴结的真菌含量检测的CT值4个时间段都小于35,且随着时间的推移,B组较A组的CT值下降明显,P0.01,而C组则较B组有明显上升,P0.05；肾脏组织中真菌含量检测到的时间A组在12小时,B组从6小时开始检测到,且较A组在同一时间段明显下降,P0.01,而C组检测到的真菌含量CT值较B组在同一时间段也是明显升高,P0.05. 结论：严重烧伤后,肠源性真菌感染的几率升高,播散到机体其它脏器的范围扩大,肝、肾功能的受损程度加重,肺部MPO和肠道HSP-70的表达增高；早期应用大黄,可以减轻烫伤大鼠的肠源性真菌感染的程度,缩小真菌向其它脏器播散的范围,并使相关指标降低。
[Abstract]:Objective: to investigate the preventive and therapeutic effects of rhubarb on intestinal fungal infection in severely burned rats. Methods: 72 healthy Wistar rats were randomly divided into three groups: group A, group B, and group C, and were fed with 7 脳 10 ~ 8 Candida albicans / ml respectively to establish intestinal fungal infection model. Group A, group B, group C, group A, group B, and group C, respectively, were perfused with 50% rhubarb solution according to 5ml/kg after 5 minutes. Then the rats were killed at different time points (6 h, 12 h, 24 h, 48 h), and the rat heart venous blood was immediately taken to detect the liver function and renal function. Myeloperoxidase (MPO) and heat shock protein-70 (HSP70) were detected by Elisa in lung and intestinal mucosa of rats in each group. The CT values of Candida albicans in lung, liver, mesenteric lymph node and kidney of rats in each group were measured by real-time fluorescence quantitative PCR. Results: (1) the levels of ALT and AST in group B were significantly higher than those in group A at the same time (P0.001). Compared with group B, the levels of BUN and Cr in group C were significantly lower than those in group B (P < 0. 01 and 0. 05, P < 0. 01 and 0. 05 respectively, P < 0. 01). Compared with group A, there was no significant difference between group B and group A in 6 hours and 12 hours, P 0.05, and no statistical significance. There was significant increase in 24 hours and 48 hours in group B, P 0.001, there was significant difference between group B and group A (P < 0.05). Compared with group B, there was no significant increase in BUN and Cr in group C at 6 h and 12 h, P 0.05, P < 0.05, P < 48 h, P < 0.05, P < 0.05. (3) the results of pulmonary MPO in group B were significantly higher than those in group A at the same time period (P0.001, P < 0.01), and there was significant difference between the two groups (P < 0.01). P0.001.4 intestinal HSP-70 results in group B were significantly higher than those in group A (P0.001 1), but in group C it was significantly lower than that in group B (P < 0.01). Group C was also higher than group B in varying degrees. P values were less than 0.05 in 6 hours and 24 hours, and less than 0.001.5 in 12 hours and 48 hours. The results of fluorescence quantitative PCR showed that Candida albicans was detected in group A at 48 hours. Candida albicans was detected in group B from the 12-hour period, and the CT values in 24-hour and 48-hour groups were lower than those in the previous one, indicating that the bacteria content was increasing continuously, and there was significant difference between group A and group A in the same period of time (P 0.01). There was no Candida albicans detected in group C with CT values greater than 35 in 6 hours and 12 hours. Compared with group B, 12 hours, 24 hours and 48 hours in group C, there was significant difference in CT values (P 0.01, 24 hours and 48 hours, P 0.05). The time period of fungus detection in liver tissue was 12 hours in group A and 6 hours in group B. Compared with group A, CT value in group B decreased significantly at the same time, P 0.01. After using rhubarb, CT value increased again, P 0.05; The CT values of fungi in mesenteric lymph nodes were all less than 35 in 4 time periods, and the CT values in group B were significantly lower than those in group A (P 0.01), while those in group C were significantly higher than those in group B (P 0.05), and the CT values in group B were significantly lower than those in group A (P 0.05). The time of detection of fungal content in kidney tissue was 12 hours in group A and 6 hours in group B, and it was significantly lower than that in group A at the same time period (P0.01, P < 0.01). The CT value of fungi detected in group C was significantly higher than that in group B at the same time (P 0.05). Conclusion: after severe burn, the incidence of intestinal fungal infection is increased, the spread to other organs of the body is expanded, the degree of liver and kidney damage is aggravated, and the expression of MPO and HSP-70 in the lungs is increased. Early application of rhubarb can reduce the degree of intestinal fungal infection in scalded rats, reduce the spread of fungi to other organs, and reduce the related indexes.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R965;R644
本文编号：2431797
[Abstract]:Objective: to investigate the preventive and therapeutic effects of rhubarb on intestinal fungal infection in severely burned rats. Methods: 72 healthy Wistar rats were randomly divided into three groups: group A, group B, and group C, and were fed with 7 脳 10 ~ 8 Candida albicans / ml respectively to establish intestinal fungal infection model. Group A, group B, group C, group A, group B, and group C, respectively, were perfused with 50% rhubarb solution according to 5ml/kg after 5 minutes. Then the rats were killed at different time points (6 h, 12 h, 24 h, 48 h), and the rat heart venous blood was immediately taken to detect the liver function and renal function. Myeloperoxidase (MPO) and heat shock protein-70 (HSP70) were detected by Elisa in lung and intestinal mucosa of rats in each group. The CT values of Candida albicans in lung, liver, mesenteric lymph node and kidney of rats in each group were measured by real-time fluorescence quantitative PCR. Results: (1) the levels of ALT and AST in group B were significantly higher than those in group A at the same time (P0.001). Compared with group B, the levels of BUN and Cr in group C were significantly lower than those in group B (P < 0. 01 and 0. 05, P < 0. 01 and 0. 05 respectively, P < 0. 01). Compared with group A, there was no significant difference between group B and group A in 6 hours and 12 hours, P 0.05, and no statistical significance. There was significant increase in 24 hours and 48 hours in group B, P 0.001, there was significant difference between group B and group A (P < 0.05). Compared with group B, there was no significant increase in BUN and Cr in group C at 6 h and 12 h, P 0.05, P < 0.05, P < 48 h, P < 0.05, P < 0.05. (3) the results of pulmonary MPO in group B were significantly higher than those in group A at the same time period (P0.001, P < 0.01), and there was significant difference between the two groups (P < 0.01). P0.001.4 intestinal HSP-70 results in group B were significantly higher than those in group A (P0.001 1), but in group C it was significantly lower than that in group B (P < 0.01). Group C was also higher than group B in varying degrees. P values were less than 0.05 in 6 hours and 24 hours, and less than 0.001.5 in 12 hours and 48 hours. The results of fluorescence quantitative PCR showed that Candida albicans was detected in group A at 48 hours. Candida albicans was detected in group B from the 12-hour period, and the CT values in 24-hour and 48-hour groups were lower than those in the previous one, indicating that the bacteria content was increasing continuously, and there was significant difference between group A and group A in the same period of time (P 0.01). There was no Candida albicans detected in group C with CT values greater than 35 in 6 hours and 12 hours. Compared with group B, 12 hours, 24 hours and 48 hours in group C, there was significant difference in CT values (P 0.01, 24 hours and 48 hours, P 0.05). The time period of fungus detection in liver tissue was 12 hours in group A and 6 hours in group B. Compared with group A, CT value in group B decreased significantly at the same time, P 0.01. After using rhubarb, CT value increased again, P 0.05; The CT values of fungi in mesenteric lymph nodes were all less than 35 in 4 time periods, and the CT values in group B were significantly lower than those in group A (P 0.01), while those in group C were significantly higher than those in group B (P 0.05), and the CT values in group B were significantly lower than those in group A (P 0.05). The time of detection of fungal content in kidney tissue was 12 hours in group A and 6 hours in group B, and it was significantly lower than that in group A at the same time period (P0.01, P < 0.01). The CT value of fungi detected in group C was significantly higher than that in group B at the same time (P 0.05). Conclusion: after severe burn, the incidence of intestinal fungal infection is increased, the spread to other organs of the body is expanded, the degree of liver and kidney damage is aggravated, and the expression of MPO and HSP-70 in the lungs is increased. Early application of rhubarb can reduce the degree of intestinal fungal infection in scalded rats, reduce the spread of fungi to other organs, and reduce the related indexes.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R965;R644
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