肾上腺髓质素在急性坏死性胰腺炎大鼠肝、肾的表达

发布时间：2019-04-10 07:17

【摘要】：目的观察肾上腺髓质素（ADM）在急性坏死性胰腺炎（ANP）并肝、肾损伤大鼠肝、肾组织中的表达情况，观察ADM在血清内含量的变化情况，探讨ADM与ANP及其合并的肝、肾损伤的关系。方法将64只SD大鼠随机分为ANP模型组（A组，n=32）和假手术对照组（C组，n=32）。A组采用经十二指肠乳头逆行胰胆管注射5%牛磺胆酸钠诱导大鼠ANP并肝、肾损伤模型。C组仅开腹后轻轻翻动胰腺。每组再分为3h、6h、12h、24h四个实验小组，每个实验小组8只SD大鼠，在术后对应时点分批处死，，取胰腺、肝及肾组织在光镜下进行组织病理学检查及病理学评分。分装血清分别应用底物酶法检测淀粉酶（AMS）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST），应用ELISA法检测血清ADM浓度。应用荧光定量PCR法检测肝、肾组织内ADM mRNA的表达。应用免疫组化法检测肝、肾组织内ADM蛋白的表达情况。结果A组大鼠胰腺病理损伤逐渐加重，病理评分逐渐增高，24h为最高值，各时点均显著高于同时点C组（P＜0.01）。A组肝脏病理评分于造模后3h开始增高，12h达峰值，各时点均高于同时点C组（P＜0.01）。A组肾脏病理评分逐渐增高，24h为最高值，各时点均高于同时点C组（P＜0.05）。A组血清AMS与AST水平于造模后3h开始升高，6h达峰值，各时点均显著高于同时点C组（P＜0.05, P＜0.01）。A组血清ALT水平逐渐升高，24h为最高值，各时点均高于同时点C组（P＜0.05）。A组血清ADM水平于造模后3h上升，12h达峰值，各时点均高于同时点C组（P＜0.05）。A组肝组织ADMmRNA表达于造模后3h升高，6h达峰值，各时点均高于同时点C组（P＜0.05）。A组肾组织ADM mRNA表达于造模后12h内升高不明显，随着时间延长而缓慢上升，造模后24h为最高值，高于同时点C组（P＜0.01)。A组肝组织于造模后3h开始出现ADM蛋白强阳性表达，24h为最高值，在3h、12h、24h时高于同时点C组（P＜0.05）。A组肾组织于造模后3h开始出现ADM蛋白强阳性表达，6h达峰值，并一直保持在高水平，各时点均高于同时点C组（P＜0.05）。结论ANP大鼠血清ADM水平升高可能与疾病严重程度有关，ADM在ANP大鼠肝、肾组织的表达增高可能与ANP合并的肝、肾损伤的严重程度有关。
[Abstract]:Objective to observe the expression of adrenomedullin (ADM) in the liver and kidney of rats with acute necrotizing pancreatitis (ANP) and renal injury, to observe the changes of ADM in serum, and to explore the relationship between ADM and ANP and their combined liver, and to observe the expression of adrenomedullin in liver and kidney of rats with acute necrotizing pancreatitis. The relationship between renal injury and renal injury. Methods Sixty-four SD rats were randomly divided into ANP model group (group A, n = 32) and sham-operated control group (group C, n = 32). ANP and liver were induced by retrograde injection of 5% sodium taurocholate into the duodenal papillary cholangiopancreatic duct in group C (n = 32). Renal injury model. Group C only slightly flipped the pancreas after laparotomy. Each group was divided into four groups (3 h, 6 h, 12 h, 24 h). Eight SD rats in each group were killed at the corresponding time point after operation. The pancreas, liver and kidney tissues were taken for histopathological examination and pathological score under light microscope. Serum (AMS), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were detected by substrate enzyme method and serum ADM concentration was determined by ELISA method. The expression of ADM mRNA in liver and kidney was detected by fluorescence quantitative PCR. Immunohistochemical method was used to detect the expression of ADM protein in liver and kidney. Results the pathological injury of pancreas in group A was gradually aggravated, and the pathological score was gradually increased, the highest value was at 24 h, which was significantly higher than that in group C at the same time point (P < 0 01). The liver pathological score of group A was increased at 3 h after modeling, and reached the peak at 12 h after the establishment of the model (P < 0 01). The levels of serum AMS and AST in group C were significantly higher than those in group C at all time points (P < 0.01), and the renal pathological scores in group C were higher than those in group C at 24 hours (P < 0.01). The levels of serum AMS and AST in group C were significantly higher than those in group C at the same time (P < 0.05). A, P < 0.05). The levels of serum ALT in group C were significantly higher than those in group C at 6 h (P < 0.05, P < 0.01), and the highest level of serum ALT was at 24 h in group C (P < 0.05, P < 0.01). The levels of serum ADM in group C were higher than those in group C at the same time point (P < 0.05), the level of serum ADM in group). A increased at 3 h, reached the peak at 12 h, and the expression of ADMmRNA in liver tissue of group C at the same time point was higher than that in group C (P < 0.05). A) at 3 h after modeling. At 6 h, the expression of ADM mRNA in renal tissue of group C was higher than that of group C at the same time (P < 0.05). The expression of ADM mRNA in renal tissue of group C was not significantly increased within 12 hours after modeling (P < 0.05), but it increased slowly with the prolongation of time, and reached the highest value at 24 hours after modeling. The expression of ADM protein in liver tissue of group C was higher than that of group C at the same time (P < 0.01). The expression of ADM protein in liver tissue of group). A was the highest at 3 h and 12 h after the establishment of the model, and it was the highest at 24 h. The expression of ADM protein in renal tissue of group C was significantly higher than that of group C (P < 0.05) at 24 h (P < 0.05), and reached the peak at 6 h after the establishment of the model (P < 0.05). The expression of ADM protein in renal tissue of group C was higher than that of group C at the same time point (P < 0.05). Conclusion the increase of serum ADM level in ANP rats may be related to the severity of the disease. The increased expression of ADM in the liver and kidney tissues of ANP rats may be related to the severity of liver and kidney injury associated with ANP.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R657.51

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