氯化钆对脓毒症大鼠急性肺损伤的肺保护作用
发布时间：2019-05-12 20:29
【摘要】：目的建立大鼠脓毒症急性肺损伤模型，探讨氯化钆（GdCl3）在脓毒症大鼠急性肺损伤中的肺保护作用及可能机制，为临床治疗脓毒症急性肺损伤提供新的理论和实验依据。 方法采用盲肠结扎穿孔术（CLP）建立脓毒症急性肺损伤模型。将36只成年Wistar大鼠采用10%水合氯醛0.3g/Kg腹腔注射麻醉后，行正中切口开腹，暴露盲肠，随即均分为假手术组(Sham组)、脓毒症组（CLP组）和氯化钆治疗组（GdCl3组），每组12只，CLP组和GdCl3组于盲肠根部丝线结扎，盲肠切口5mm，肠内容物漏出，将其回纳入腹腔，关腹。假手术组大鼠仅翻动盲肠，不结扎穿孔。GdCl3组术后经尾静脉注射2%GdCl3溶液（10mg.kg-1）。实验结束后，，应用酶联免疫法（ELISA）法测定血浆中肿瘤坏死因子α（TNF-α）水平，应用硫代巴比妥酸（TBA）法测定丙二醛(MDA)水平，测定肺湿／干重比值（W/D）,部分肺组织用HE染色法染色并在光镜下观察肺组织病理结构的改变，统计方法采用SPSS19.0。 结果与Sham组相比， CLP组、GdCl3组血浆TNF-α、MDA水平，W/D明显升高，差异有统计学意义（P＜0.05）；与CLP组相比， GdCl3组血浆TNF-α、MDA水平，W/D明显降低，差异有统计学意义（P＜0.05）;肺病理提示CLP组和GdCl3组肺泡间隔及肺泡内炎性细胞浸润、出血， GdCl3组肺组织病理学改变明显减轻。 结论急性肺损伤时，血浆TNF-α、MDA水平明显升高，GdCl3可通过抑制巨噬细胞释放的TNF-α等炎性介质来减轻脓毒症所致的急性肺损伤的炎症反应。
[Abstract]:Objective to establish a rat model of acute lung injury induced by sepsis and to explore the protective effect and possible mechanism of gadolinium chloride (GdCl3) in acute lung injury in septic rats, so as to provide a new theoretical and experimental basis for clinical treatment of acute lung injury with sepsis. Methods the acute lung injury model of sepsis was established by cecal ligation and perforation (CLP). Thirty-six adult Wistar rats were anesthetized with 10% chloral hydrate 0.3g/Kg intraabdominal injection, then underwent median incision laparotomy and exposed cecum. They were divided into three groups: sham operation group (Sham group), sepsis group (CLP group) and gadolinium chloride treatment group (GdCl3 group). In each group, 12 rats in CLP group and GdCl3 group were ligated at the root of cecum, the cecum incision was 5 mm, the intestinal contents leaked out, and the contents of the intestine were returned to the abdominal cavity and closed the abdomen. Rats in the sham operation group only turned the cecum without ligating and perforation. 2%GdCl3 solution (10mg.kg-1) was injected intravenously through the tail vein after operation in the GdCl3 group. At the end of the experiment, the level of tumor necrosis factor 伪 (TNF- 伪) in plasma was measured by enzyme linked immunosorbent assay (Elisa), the level of malondialdehyde (MDA) (MDA) was measured by thiobarbituric acid (TBA) method, and the lung wet / dry weight ratio (W 鈮
本文编号：2475681
[Abstract]:Objective to establish a rat model of acute lung injury induced by sepsis and to explore the protective effect and possible mechanism of gadolinium chloride (GdCl3) in acute lung injury in septic rats, so as to provide a new theoretical and experimental basis for clinical treatment of acute lung injury with sepsis. Methods the acute lung injury model of sepsis was established by cecal ligation and perforation (CLP). Thirty-six adult Wistar rats were anesthetized with 10% chloral hydrate 0.3g/Kg intraabdominal injection, then underwent median incision laparotomy and exposed cecum. They were divided into three groups: sham operation group (Sham group), sepsis group (CLP group) and gadolinium chloride treatment group (GdCl3 group). In each group, 12 rats in CLP group and GdCl3 group were ligated at the root of cecum, the cecum incision was 5 mm, the intestinal contents leaked out, and the contents of the intestine were returned to the abdominal cavity and closed the abdomen. Rats in the sham operation group only turned the cecum without ligating and perforation. 2%GdCl3 solution (10mg.kg-1) was injected intravenously through the tail vein after operation in the GdCl3 group. At the end of the experiment, the level of tumor necrosis factor 伪 (TNF- 伪) in plasma was measured by enzyme linked immunosorbent assay (Elisa), the level of malondialdehyde (MDA) (MDA) was measured by thiobarbituric acid (TBA) method, and the lung wet / dry weight ratio (W 鈮
本文编号：2475681
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