Th17细胞及白细胞介素-17与肝移植急性排斥反应的相关性研究
发布时间：2019-05-16 17:58
【摘要】：目的研究Thl7细胞及其相关因子白细胞介素-17（IL-17）于肝移植急性排斥反应中的临床作用及意义。 方法收集2011年1月至2012年12月于大连医科大学附属第二医院行肝移植手术的患者28例，然后根据移植肝组织病理穿刺活检诊断结果将进行肝移植的28例患者分为两组，分别为：急性排异反应组(急性排斥组，6例)和无排斥反应的稳定组(稳定组，，22例)，另外选取15名健康体检者作为健康对照组。急性排斥组及稳定组在移植术后3天和7天，行肝穿刺活检病理检查；同时用流式细胞仪检测三组受检者外周血中CD45+T淋巴细胞中Th17细胞比例，用酶联免疫吸附实验（ELISA）检测三组受检者血清中IL-17含量。 结果 1.移植肝组织病理穿刺活检诊断显示急性排斥组中的患者，随着移植术后时间的延长，排斥反应会逐渐增强，且术后7天的病理改变结果显示较术后3天的病理改变严重。 2.急性排斥组患者外周血中Th17细胞比例在术后3天及7天较稳定组及健康对照组均明显增多（P＜0.05），且术后7天Th17细胞比例明显高于3天（P＜0.05）；稳定组和健康对照组比较无明显差别(P＞0.05)。 3.急性排斥组术后3天及7天，患者血清中IL-17含量较稳定组及健康对照组均有明显升高（P＜0.05），且术后7天IL-17含量明显高于3天（P＜0.05）； 4.稳定组患者血清中的IL-17含量于术后3天及7天较健康对照组相比明显升高(P＜0.05)，但术后7天IL-17含量与术后3天相比无明显差别（P＞0.05）。 结论Thl7细胞及其相关因子白细胞介素-17（IL-17）在肝移植急性排斥反应的发生、发展中可能起着极其重要的作用，对肝移植患者外周血中Th17细胞及IL-17的检测可作为肝移植急性排斥反应早期诊断的指标。
[Abstract]:Objective to study the clinical role and significance of Thl7 cells and its related factor IL-17 (IL-17) in acute rejection of liver transplantation. Methods from January 2011 to December 2012, 28 patients undergoing liver transplantation in the second affiliated Hospital of Dalian Medical University were collected, and then 28 patients undergoing liver transplantation were divided into two groups according to the diagnosis results of pathological puncture and biopsies of liver tissue. Acute rejection group (acute rejection group, n = 6) and stable group without rejection (stable group, n = 22). Another 15 healthy subjects were selected as healthy control group. Liver biopsies were performed in acute rejection group and stable group 3 and 7 days after transplantation. At the same time, the proportion of Th17 cells in CD45 T lymphocytes in peripheral blood of the three groups was detected by flow cytometry, and the content of IL-17 in serum of the three groups was detected by enzyme-linked immunosorbent assay (ELISA). Result 1. Pathological biopsies of liver transplantation showed that the rejection of patients in acute rejection group increased gradually with the prolongation of transplantation time, and the pathological changes of 7 days after operation were more serious than those of 3 days after operation. 2. The proportion of Th17 cells in peripheral blood of patients with acute rejection was significantly higher than that of stable group and healthy control group on the 3rd and 7th day after operation, and the proportion of Th17 cells on the 7th day after operation was significantly higher than that on the 3rd day (P < 0.05). There was no significant difference between the stable group and the healthy control group (P > 0.05). 3. The content of IL-17 in serum of acute rejection group was significantly higher than that of stable group and healthy control group on the 3rd and 7th day after operation, and the content of IL-17 on the 7th day after operation was significantly higher than that on the 3rd day (P < 0.05). The serum IL-17 content in the stable group was significantly higher than that in the healthy control group on the 3rd and 7th day after operation, but there was no significant difference in IL-17 content between the stable group and the healthy control group on the 7th day after operation (P > 0.05). Conclusion Thl7 cells and its related factor IL-17 (IL-17) may play an important role in the occurrence and development of acute rejection in liver transplantation. The detection of Th17 cells and IL-17 in peripheral blood of patients with liver transplantation can be used as an index for early diagnosis of acute rejection of liver transplantation.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R657.3
本文编号：2478462
[Abstract]:Objective to study the clinical role and significance of Thl7 cells and its related factor IL-17 (IL-17) in acute rejection of liver transplantation. Methods from January 2011 to December 2012, 28 patients undergoing liver transplantation in the second affiliated Hospital of Dalian Medical University were collected, and then 28 patients undergoing liver transplantation were divided into two groups according to the diagnosis results of pathological puncture and biopsies of liver tissue. Acute rejection group (acute rejection group, n = 6) and stable group without rejection (stable group, n = 22). Another 15 healthy subjects were selected as healthy control group. Liver biopsies were performed in acute rejection group and stable group 3 and 7 days after transplantation. At the same time, the proportion of Th17 cells in CD45 T lymphocytes in peripheral blood of the three groups was detected by flow cytometry, and the content of IL-17 in serum of the three groups was detected by enzyme-linked immunosorbent assay (ELISA). Result 1. Pathological biopsies of liver transplantation showed that the rejection of patients in acute rejection group increased gradually with the prolongation of transplantation time, and the pathological changes of 7 days after operation were more serious than those of 3 days after operation. 2. The proportion of Th17 cells in peripheral blood of patients with acute rejection was significantly higher than that of stable group and healthy control group on the 3rd and 7th day after operation, and the proportion of Th17 cells on the 7th day after operation was significantly higher than that on the 3rd day (P < 0.05). There was no significant difference between the stable group and the healthy control group (P > 0.05). 3. The content of IL-17 in serum of acute rejection group was significantly higher than that of stable group and healthy control group on the 3rd and 7th day after operation, and the content of IL-17 on the 7th day after operation was significantly higher than that on the 3rd day (P < 0.05). The serum IL-17 content in the stable group was significantly higher than that in the healthy control group on the 3rd and 7th day after operation, but there was no significant difference in IL-17 content between the stable group and the healthy control group on the 7th day after operation (P > 0.05). Conclusion Thl7 cells and its related factor IL-17 (IL-17) may play an important role in the occurrence and development of acute rejection in liver transplantation. The detection of Th17 cells and IL-17 in peripheral blood of patients with liver transplantation can be used as an index for early diagnosis of acute rejection of liver transplantation.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R657.3
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