光动力治疗大鼠大面积创伤感染的实验研究

发布时间：2019-06-14 16:46

【摘要】：探讨新型卟啉类光敏剂PA1介导的光动力抗菌疗法治疗大鼠大面积创伤感染的效果。体外培养创面易感染的3种菌株,有抗药性金黄色葡萄球菌(MRSA)、大肠杆菌(E.coli)和铜绿假单胞菌(P.aeruginosa),探讨3种细菌对PA1的吸收量、光照PA1的最小抑菌浓度和最低杀菌浓度;取清洁级雄性Wistar大白鼠40只,建立皮肤大面积创伤感染模型,创面滴加光敏剂PA1,用650 nm激光照射治疗,检测各实验组的创面愈合率、不同时相点创面下的菌落数。结果表明,上述3种细菌对PA1的最大吸收量都发生于前30 min,以后趋于平稳。光照PA1对3种细菌均有灭活作用,对MRSA和E.coli的最低杀菌浓度为31μM,对P.aeruginosa的最低杀菌浓度为62.5μM。体内实验结果显示,治疗组的各项指标优于模型对照组(P0.05或P0.01),高剂量治疗组的各项指标优于中、低量治疗组(P0.01),为临床治疗大面积创伤感染积累一定的实验经验。
[Abstract]:Objective To study the effect of novel photodynamic antibacterial therapy (PA1)-mediated photodynamic therapy (PA1) in the treatment of large-area wound infection in rats. Three strains which were susceptible to infection were cultured in vitro, and there were drug-resistant Staphylococcus aureus (MRSA), Escherichia coli (E. coli) and P. aeruginosa. The absorption of PA1 and the minimum inhibitory concentration and the minimum bactericidal concentration of PA1 were discussed. 40 rats of clean-grade male Wistar rats were used to establish a large-area wound infection model of the skin, and a photosensitizer PA1 was added dropwise to the wound surface, and the treatment was irradiated with 650nm laser, and the wound healing rate of each experimental group was detected, and the number of colonies under the wound surface was not at the same time. The results showed that the maximum absorption of PA1 in the above three kinds of bacteria occurred in the first 30 minutes, and then it was stable. The results of the in-vivo experiment showed that all the indexes of the treatment group were superior to that of the model control group (P0.05 or P0.01), and the indexes of the high-dose treatment group were better than that of the model control group (P0.05 or P0.01). The low-dose treatment group (P0.01) has a certain experimental experience for clinical treatment of large-area wound infection.
【作者单位】：河北大学基础医学院;河北大学公共卫生学院;河北大学附属医院;中国医学科学院生物医学工程研究所;
【基金】：天津市重大科技支撑项目(12ZCDZSY11900) 河北大学医学学科建设项目(2015A2001);河北大学大学生创新训练计划项目(2015149)
【分类号】：R641

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