海马齿状回GABAB受体在血管性痴呆大鼠空间学习记忆中的作用

发布时间：2018-01-01 12:05

本文关键词：海马齿状回GABAB受体在血管性痴呆大鼠空间学习记忆中的作用　出处：《中国老年学杂志》2015年16期 　论文类型：期刊论文

【摘要】：目的探讨海马齿状回(DG)的GABAB受体是否参与血管性痴呆(VD)大鼠空间学习记忆损害。方法 SD雄性大鼠24只,随机分为假手术组、VD组及GABAB受体阻断剂沙克洛芬组,双侧颈总动脉永久性结扎法制备VD大鼠模型。利用Morris水迷宫检测大鼠的空间学习记忆能力;脑部微量透析法和高效液相色谱法测定DG区γ-氨基丁酸(GABA)含量;免疫组织化学法观察DG区GABAB受体表达;用脑部微量注射法往DG区注射GABAB受体阻断剂沙克洛芬,观察其对VD模型大鼠空间学习记忆的影响。结果 VD大鼠海马DG区GABA含量明显增加,而GABAB受体表达减少(均为P0.05)。VD大鼠的空间学习记忆能力显著下降,而训练前向DG区微量注射GABAB受体阻断剂可改善VD大鼠的空间学习记忆损害。结论 VD模型大鼠海马DG内增加的GABA通过GABAB受体参与其空间学习记忆的损害。
[Abstract]:Objective to investigate the dentate gyrus (DG) whether the GABAB receptor is involved in vascular dementia (VD) rats learning and memory impairment. Methods 24 male SD rats were randomly divided into sham operation group, VD group and GABAB receptor antagonist group Shah Chloe Finn, permanent bilateral common carotid artery ligation model was prepared by the method of VD the ability of learning and memory in rats. Morris water maze test in rats; Determination of DG gamma aminobutyric acid microdialysis and high performance liquid chromatography (GABA) content; immunohistochemical method to observe the expression of DG GABAB receptor in the brain; microinjection into DG microinjection of GABAB receptor antagonist Shah Chloe Finn, observation the changes of learning and memory in VD rats. The content of GABA in hippocampus DG area of VD rats increased significantly, and reduce the expression of GABAB receptor (P0.05).VD spatial learning and memory ability in rats decreased significantly, while training the DG prior to microinjection GABAB receptor antagonist can improve the spatial learning and memory impairment of VD rats. Conclusion GABA increased in hippocampus DG of VD rats is involved in spatial learning and memory impairment through GABAB receptor.

【作者单位】：延边大学附属医院外科;延边大学医学院生理学与病理生理学教研室;
【基金】：国家自然科学基金(31160211) 延边大学自然科学基金(602013110)
【分类号】：R749.1
【正文快照】： 血管性痴呆(VD)是本文常见的与年龄相关的神经功能障碍〔1〕,海马是学习记忆的关键部位,尤其在空间学习记忆中起重要作用〔2〕。哺乳类海马齿状回(DG)作为信息进入海马的传入点,对空间信息进行处理和编码,并在空间学习记忆中起着至关重要的作用〔3〕。研究表明,海马DG在VD空间

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