CUS致大鼠抑郁行为涉及TNF-α及IDO-HAAO通路激活

发布时间：2018-01-02 03:34

本文关键词：CUS致大鼠抑郁行为涉及TNF-α及IDO-HAAO通路激活　出处：《中国药理学通报》2016年05期 　论文类型：期刊论文

【摘要】：目的研究慢性不可预见刺激(chronically unpredictable stress,CUS)致大鼠抑郁行为与肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)、吲哚胺2,3-双加氧酶(indole-2,3-dioxygenase,IDO)及3-羟氨苯甲酸双加氧酶(3-hydroxyl amino acid oxygenase,HAAO)蛋白表达的关系。方法♂Sprague Dawley(SD)大鼠30只,随机分为对照组和模型组,采用孤养结合CUS连续刺激28 d建立大鼠抑郁症模型;以旷场实验和被动游泳实验评价大鼠抑郁行为,以Western blot法检测大鼠前脑皮层和海马TNF-α、IDO及HAAO蛋白表达。结果与对照组大鼠相比,模型组被动游泳实验不动时间明显延长(P0.01)、旷场实验得分明显降低(P0.01)、前脑皮层和海马的TNF-α、IDO及HAAO蛋白表达均明显升高(P0.05)。结论 CUS诱导大鼠产生抑郁行为,其机制可能与激活脑内TNF-α及下游IDO-HAAO通路有关。
[Abstract]:Objective to study chronic unpredictable stimuli chronically unpredictable stress. CUS-induced depression behavior and tumor necrosis factor 伪 -tumor necrosis factor alpha-TNF- 伪, indoleamine 2 in rats. 3-dioxygenase. Ido) and 3-hydroxyl amino acid oxygenase 3-hydroxyl acid oxygenase 3-hydroxyl acid of 3-hydroxyaminobenzoate. 鈾俆hirty SD rats of Sprague Dawley's disease were randomly divided into two groups: control group and model group. The depression model was established by the combination of solitary nourishment and continuous stimulation of CUS for 28 days. Open field test and passive swimming test were used to evaluate the depressive behavior of rats, and Western blot method was used to detect TNF- 伪 in the forebrain cortex and hippocampus of rats. Results compared with the control group, the immobility time of the passive swimming test in the model group was significantly longer than that in the control group, and the score of the open field test was significantly lower than that of the control group (P 0.01). The expression of TNF- 伪 nido and HAAO protein in forebrain cortex and hippocampus increased significantly (P 0.05). Conclusion CUS induces depression behavior in rats. The mechanism may be related to activation of TNF- 伪 and downstream IDO-HAAO pathway.
【作者单位】：重庆医科大学药理学教研室重庆市生物化学与分子药理学重点实验室;
【基金】：国家自然科学基金资助项目(No 31400881) 重庆市教委科学技术项目(No KJ1400208)
【分类号】：R749.4
【正文快照】： 抑郁症是一种临床常见的精神障碍性疾病,以少动、思维缓慢、兴趣缺失、记忆力减退等为主要特征,严重者甚至出现自杀倾向[1]。抑郁症发病机制复杂,涉及遗传、环境、表观遗传学等众多因素。研究显示,单胺类神经递质系统功能紊乱、下丘脑-垂体-肾上腺轴功能失调、脑源性神经营养

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