S100A16在抑郁症中的作用及其机制研究

发布时间：2018-01-09 10:20

本文关键词：S100A16在抑郁症中的作用及其机制研究　出处：《南京医科大学》2015年硕士论文　论文类型：学位论文

更多相关文章： S100A16 抑郁症 机制 生物学功能

【摘要】：目的：探讨S100A16在抑郁症中的作用,进一步揭示抑郁症发病的分子机制及S100A16的生物学功能。方法：选用成年雄性B6小鼠30只,随机分成正常对照组和抑郁症组,每组各15只。采用孤养和慢性不可预知的温和应激(chronic unpredictable mild stress, CMS)方法制备抑郁小鼠模型,每周观察小鼠体重、皮毛评分。抑郁模型制备5周后评价模型的有效性。在确定模型建立成功后,采用酶联免疫吸附试验观察S100A16蛋白在小鼠血清中的变化。用蛋白质印记(WB)法检测S100A16及相关蛋白在小鼠海马组织中的表达情况。同时收集42例抑郁症患者及55例健康人的血清,观察血脂情况和血清中S100A16的表达情况,分析抑郁症患者血脂和血清中S100A16的变化。结果：(1)在孤养和慢性不可预知的温和刺激5周后,抑郁组小鼠体重由初始21±0.69g降至17.488±1.08g;皮毛污秽,皮毛评分为1分；强迫游泳实验中抑郁症小鼠的游泳不动时间为113.1±28.47sec.较正常对照组小鼠不动时间为59.4±25.65sec.明显延长；蔗糖偏爱试验正常对照组的蔗糖偏爱率为0.905±0.117,模型组小鼠为0.187±0.167,二者差异明显具有统计学意义。抑郁症组小鼠血清中的S100A16水平为2891.578±822.972ng/ml低于正常对照组8775.6524±1919.809 ng/ml并具有明显统计学意义(p0.001)。(2)采用汉密尔顿抑郁量表(HAMD)评定抑郁症患者的抑郁症状。分析比较抑郁症患者与健康人群的血脂,发现抑郁症患者的TC 4.46±0.79mmol/L较正常人群4.86±0.68mmol/L下降；HDL-C为1.23±0.25 mmol/L低于健康人群1.4±0.26 mmol/L,二者差值有统计学意义,p0.05；而抑郁症患者的甘油三酯(TG)的均值1.35±0.80mmol/L显著高于正常对照组0.95±0.33mmol/L,p0.05统计学差异明显；分析血清S100A16蛋白浓度与疾病状况间的关系,发现抑郁症患者血清中S100A16浓度3.15±2.19ng/ml较正常对照人群6.13±2.68ng/ml下降,有统计学意义(p0.05)。结论：S100A16与慢性温和应激所诱发的抑郁症密切相关,具体的机制还需进一步的研究。
[Abstract]:Objective: to explore the role of S100A16 in depression and to reveal the molecular mechanism of depression and the biological function of S100A16. Methods: 30 adult male B6 mice were selected. They were randomly divided into normal control group and depression group. 15 rats in each group were treated with chronic unpredictable mild stress and chronic unpredictable mild stress. The weight and fur score of the mice were observed weekly. The effectiveness of the depression model was evaluated after 5 weeks. After the establishment of the model, the model was established successfully. The changes of S100A16 protein in mouse serum were observed by enzyme linked immunosorbent assay (Elisa). Methods the expression of S100A16 and related proteins in the hippocampus of mice was detected, and the serum samples of 42 patients with depression and 55 healthy persons were collected at the same time. The blood lipid and the expression of S100A16 in serum were observed. The changes of blood lipids and serum S100A16 in patients with depression were analyzed. Results: 1) after 5 weeks of solitary and chronic unpredictable mild stimulation. The body weight of depression group decreased from 21 卤0.69 g to 17.488 卤1.08 g; Dirty fur, fur score 1; In forced swimming experiment, the immobility time of depression mice was 113.1 卤28.47 sec.The immobility time of depression mice was 59.4 卤25.65 sec. significantly longer than that of normal control mice. The sucrose preference rate was 0.905 卤0.117 in the normal control group and 0.187 卤0.167 in the model group. The serum S100A16 level in depression group was 2891.578 卤822.972 ng / ml lower than that in normal control group 8775.652. 4 卤1919.809. Ng/ml has significant statistical significance (. The depression symptoms were assessed by Hamilton Depression scale (Hamd). The TC of depression patients was 4.46 卤0.79 mmol / L lower than that of normal subjects (4.86 卤0.68 mmol / L). The HDL-C was 1.23 卤0.25 mmol/L lower than that of healthy subjects (1.4 卤0.26 mmol / L, P < 0.05). The mean value of triglyceride TGin in patients with depression was 1.35 卤0.80 mmol / L, significantly higher than that in normal controls (0.95 卤0.33 mmol / L). P0.05 statistical difference was significant; The relationship between serum S100A16 protein concentration and disease status was analyzed. It was found that the serum S100A16 concentration in depression patients was 3.15 卤2.19ng / ml lower than that in normal controls (6.13 卤2.68ng / ml). Conclusion: S100A16 is closely related to depression induced by chronic mild stress, and the specific mechanism needs further study.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R749.4

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