不同程度间歇性低氧大鼠学习记忆及皮质和海马区神经细胞凋亡的变化

发布时间：2018-01-09 21:58

本文关键词：不同程度间歇性低氧大鼠学习记忆及皮质和海马区神经细胞凋亡的变化　出处：《中国老年学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的复制不同程度间歇性低氧动物模型,观察神经细胞形态结构及神经细胞凋亡变化,探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)导致认知障碍的机制。方法 60只雄性SD大鼠随机分成对照组(n=20)、轻度间歇低氧组(n=20)、重度间歇低氧组(n=20)。对照组暴露于空气中,间歇低氧组分别暴露于不同低氧条件下(100 ml/L和50 ml/L,暴露时间每天8 h,持续时间2、4、6、8 w)。Morris水迷宫检测学习记忆功能,光镜和电镜观察神经细胞超微结构,原位缺口末端标记法(TUNEL)检测凋亡细胞。结果与对照组比较,随低氧时间的延长,两间歇性低氧组大鼠神经元结构损伤;TUNEL阳性细胞增多(P0.05);水迷宫检测动物逃避潜伏期时间延长、穿台次数减少(P0.05);上述变化在重度间歇性低氧组最为显著(P0.05);轻度间歇性低氧组TUNEL阳性细胞6 w达高峰,组内各时间差异显著(P0.05);重度间歇性低氧组TUNEL阳性细胞8 w达高峰,组内各时间差异显著(P0.05)。结论间歇性低氧可导致大鼠认知功能障碍,其机制与神经细胞凋亡有关,且认知损伤程度和神经细胞凋亡与间歇性低氧的时间和程度有关。
[Abstract]:Objective to make intermittent hypoxic animal models to observe the morphological structure and apoptosis of nerve cells. To investigate the mechanism of cognitive impairment induced by obstructive sleep apnea hypopnea syndrome (OSAHS). Methods 60 male Sprague-Dawley rats were randomly divided into control group (n = 20). The control group was exposed to air. The intermittent hypoxia group was exposed to 100 ml/L and 50 ml / L hypoxia, respectively. The exposure time was 8 hours per day and lasted for 2 minutes and 4 minutes. The learning and memory function was measured by Morris water maze, and the ultrastructure of nerve cells was observed by light microscope and electron microscope. The apoptotic cells were detected by Tunel in situ Nick end labeling. Results compared with the control group, with the prolongation of hypoxic time, the neuronal structure damage was observed in the two intermittent hypoxia groups. The number of TUNEL positive cells increased (P 0.05). In water maze test, the time of escape latency was prolonged, and the frequency of platform penetration was decreased (P 0.05). These changes were most significant in severe intermittent hypoxia group (P 0.05). In the mild intermittent hypoxia group, the TUNEL positive cells reached the peak at 6 weeks, and the difference in each time was significant (P 0.05). The TUNEL positive cells in severe intermittent hypoxia group reached the peak at 8 weeks, and the difference in each time was significant (P 0.05). Conclusion intermittent hypoxia can lead to cognitive dysfunction in rats. The mechanism is related to apoptosis of nerve cells, and the degree of cognitive damage and apoptosis of neurons is related to the time and degree of intermittent hypoxia.
【作者单位】：河北联合大学康复医学院;河北联合大学附属医院;
【基金】：河北省科技厅资助项目(No.09276103D-11)
【分类号】：R766;R749
【正文快照】： 阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者睡眠时反复出现不同程度的低氧血症和(或)高碳酸血症,最终引起全身多系统、多器官的渐进性损害。临床资料显示OSAHS可造成患者警觉、执行能力和运动协调方面的认知障碍〔1,2〕。皮质和海马是脑内与认知和记忆等高级功能密切相关的重

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