松果菊苷对阿尔采末病大鼠海马、皮质内神经递质水平的影响

发布时间：2018-02-02 07:46

本文关键词： 松果菊苷阿尔采末病水迷宫微透析 HPLC-ECD 单胺类神经递质　出处：《中国药理学通报》2014年11期 　论文类型：期刊论文

【摘要】：目的研究松果菊苷(ECH)对阿尔采末病(AD)模型大鼠海马、大脑皮质细胞外液中单胺类神经递质水平的影响,为ECH改善学习记忆力提供理论依据。方法 60只SD大鼠随机分为模型组、ECH低、中、高剂量组、阳性药石杉碱甲组以及假手术组。采用腹腔内注射D-半乳糖,并在右侧海马内注射Aβ25-35复制AD模型。Morris水迷宫评价各组大鼠学习记忆能力,应用脑双位点双通道同步微透析采样技术,联合运用高效液相-电化学法测定大鼠海马和皮质细胞外液中NA、DA、5-HT的含量。结果水迷宫结果显示,与假手术组相比,AD模型组大鼠逃避潜伏期明显延长(P0.05),停留在原平台象限时间明显缩短(P0.05);与模型组相比,ECH低、中、高剂量组均使大鼠学习记忆能力有不同程度的提高,ECH组逃避潜伏期明显缩短(P0.05),停留在原平台象限时间明显延长(P0.05)。同时,模型组较假手术组大脑皮质、海马中的NA、DA、5-HT明显降低(P0.05);ECH组较模型组脑内单胺类神经递质水平恢复至接近正常水平。结论 ECH可以改善AD大鼠学习记忆力,并且明显升高海马、皮质细胞外液中单胺类神经递质的含量,具有一定的抗AD作用。并且ECH中、高剂量组、阳性药石杉碱甲组在提高学习记忆能力方面作用优于ECH低剂量组。
[Abstract]:Objective to study the effect of ECH on the level of monoamine neurotransmitters in extracellular fluid of hippocampus and cerebral cortex of rats with Alzheimer's disease (ADD). Methods 60 Sprague-Dawley rats were randomly divided into three groups: model group, low dose group, middle dose group and high dose group. Positive drug Huperzine A group and sham operation group were injected intraperitoneally with D-galactose. The AD model was induced by right hippocampal injection of A 尾 25-35. Morris water maze was used to evaluate the learning and memory ability of rats in each group. The content of NAVDA 5-HT in rat hippocampal and cortical extracellular fluid was determined by high performance liquid-electrochemical method. Results the results of water maze showed that compared with sham operation group. In AD model group, the latency of escape was significantly prolonged and the time of staying in the original platform quadrant was significantly shortened. Compared with the model group, the ECH in the middle and high dose groups increased the learning and memory ability of the rats in varying degrees. The escape latency of the ECH group was significantly shortened (P 0.05). The time of staying in the original platform quadrant was significantly prolonged (P 0.05). At the same time, the cerebral cortex in the model group was significantly lower than that in the sham operation group, and the 5-HT in the hippocampus of the model group was significantly lower than that in the sham operation group (P 0.05). Compared with the model group, the level of monoamine neurotransmitters in the ECH group returned to the normal level. Conclusion ECH can improve the learning and memory of AD rats and increase the hippocampus significantly. The content of monoamine neurotransmitters in extracellular fluid of cortex has a certain anti-AD effect, and in ECH, high dose group. The effect of positive Huperzine A group on improving learning and memory ability was better than that of ECH low dose group.
【作者单位】：石河子大学药学院教育部新疆特种植物药资源重点实验室;北京大学中医药现代研究中心;
【基金】：国家自然科学基金资助项目(No 30860334,30560171)
【分类号】：R749.16
【正文快照】： R745.7;R977.1阿尔采末病(Alzheimer’s disease,AD)是一种不可逆转的、进行性的慢性神经退行性疾病,由多种原因引发,其中与海马和皮质区的神经元异常密切相关。研究表明,AD病变物质基础与人体某些对学习和记忆等认知功能有特殊作用的神经递质,如乙酰胆碱、单胺类、氨基酸类及

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