染料木素对阿尔茨海默病模型大鼠海马区神经递质含量的影响

发布时间：2018-02-24 17:33

本文关键词： 神经递质卵巢切除 β-雌二醇染料木素　出处：《中国老年学杂志》2014年17期 　论文类型：期刊论文

【摘要】：目的探讨染料木素对阿尔茨海默病模型大鼠学习记忆能力及海马区神经递质含量的影响。方法健康雌性SD大鼠,随机分为5组:假手术组(Sham)、卵巢切除组(Ovx)、卵巢切除+17β-雌二醇组(Ovx+Est)、卵巢切除+17β-雌二醇+染料木素1.0组(Ovx+Est+Gen1.0)、卵巢切除+17β-雌二醇+染料木素10.0组(Ovx+Est+Gen10.0)各6只,给药21 d后取大鼠脑海马分别测定乙酰胆碱(Ach)、去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)的含量。结果 Ovx组Ach、NE、DA及5-HT递质含量与Sham组比较显著下降(P0.05);Ovx+Est组Ach、NE、DA及5-HT递质含量与Ovx组比较显著上升(P0.05);Ovx+Est+Gen1.0组Ach、NE、DA及5-HT递质含量与Ovx+Est组相比明显下降(P0.05),Ovx+Est+Gen10.0组变化与Ovx+Est相似,两组相比无统计学意义。结论雌激素去除降低了脑部主要中枢神经递质的含量,外源性17β-雌二醇可升高中枢神经递质的含量,小剂量染料木素可以拮抗17β-雌二醇的这种作用,高剂量的染料木素对17β-雌二醇的作用无影响。
[Abstract]:Objective to investigate the effects of genistein on learning and memory ability and hippocampal neurotransmitter content in Alzheimer's disease model rats. They were randomly divided into 5 groups: sham-operated group, ovariectomized group, ovariectomized 17 尾 -estradiol group, ovx Est gen1.0 group, ovariectomized 17 尾 -estradiol gen1.0 group and ovariectomized 17 尾 -estradiol gen10.0 group (Ovx Est Gen10.0). The contents of acetylcholine acetylcholine acetylcholine (ache), norepinephrine (NE), dopamine (DA) 5-hydroxytryptamine (5-HT) were measured 21 days later. Results the contents of ache NEDA and 5-HT transmitters in the Ovx group were significantly lower than those in the Sham group, and the contents of AchNEDA and 5-HT transmitters in the Est group were significantly lower than those in the Sham group. Compared with Ovx group, the contents of ACTA and 5-HT transmitters in Ovx Est Gen1.0 group were significantly lower than those in Ovx Est group. The changes of Est Gen10.0 group were similar to those of Ovx Est group. Conclusion estrogen removal can decrease the content of major central neurotransmitters in brain, exogenous 17 尾 -estradiol can increase the content of neurotransmitters, and low dose genistein can antagonize the effect of 17 尾 -estradiol. High dose genistein had no effect on 17 尾 -estradiol.
【作者单位】：青海大学医学院人体解剖学教研室;
【基金】：国家自然科学基金项目(No.30960107) 青海大学医学院中青年科技项目(2013-KY-6) 青海大学中青年科技项目(2007-ky-003)
【分类号】：R749.16

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