环境对转基因小鼠抑郁样行为的影响

发布时间：2018-02-25 01:34

本文关键词： 母婴分离慢性不可预知轻度应激糖水消耗实验强迫游泳实验抑郁症　出处：《中国神经精神疾病杂志》2014年09期 　论文类型：期刊论文

【摘要】：目的探讨环境因素对精神分裂症断裂基因1(disruptedinschizophrenia1,DISC1)转基因小鼠抑郁样行为的影响。方法 C57BL/6野生型新生小鼠及C57BL/6 LBD-DISC1转基因新生小鼠各16只,分别随机分为野生实验组、野生对照组和DISC1实验组、DISC1对照组,每组8只。实验组小鼠在哺乳期进行6 d母婴分离,成年早期给予21 d慢性不可预知轻度应激,采用糖水消耗实验及强迫游泳实验测定四组小鼠行为,并比较各组小鼠的体质量改变。结果应激和DISC1缺陷对小鼠糖水偏好度没有交互作用(P0.05),DISC1实验组糖水偏好度低于其余三组(均P0.01);应激和DISC1缺陷对小鼠强迫游泳实验的静止时间有交互作用(P=0.010),DISC1实验组小鼠强迫游泳的静止时间长于野生对照组和DISC1对照组(均P0.01);应激和DISC1缺陷对体质量改变亦没有交互作用(P0.05),但DISC1实验组低于其余三组(均P0.01)。结论应激和DISC1缺陷共同作用导致小鼠出现抑郁样行为。
[Abstract]:Objective to investigate the effects of environmental factors on depressive behavior in transgenic mice with schizophrenia breakage gene 1 disruptedinschizophrenia 1 (DISC1). Methods 16 C57BL / 6 wild-type newborn mice and 16 C57BL / 6 LBD-DISC1 transgenic newborn mice were randomly divided into wild experimental groups. There were 8 mice in each group in the wild control group and the DISC1 experimental group. The mice in the experimental group were separated from each other for 6 days during lactation and were given 21 days of chronic unpredictable mild stress in the early adulthood. The behavior of four groups of mice was measured by sugar water consumption test and forced swimming test. Results there was no interaction between stress and DISC1 deficiency on the preference degree of sugar water in mice. Results the degree of sugar water preference of experimental group was lower than that of the other three groups (all P 0.01), and the effect of stress and DISC1 deficiency on forced swimming of mice was lower than that of other three groups. The static time of forced swimming in the experimental group was longer than that in the wild control group and the DISC1 control group (all P 0.01), and there was no interaction between stress and DISC1 deficiency on the body mass, but the DISC1 group was lower than the other three groups. Conclusion stress and DISC1 deficiency lead to depressive behavior in mice.
【作者单位】：上海交通大学医学院附属精神卫生中心;上海市重性精神病重点实验室;上海交通大学精神疾病转化医学研究重点实验室;
【基金】：上海市科技发展基金实验动物研究项目(编号:12140904200) 国家自然科学基金(编号:81171266;81271481) 上海市重性精神病重点实验室(编号:13dz2260500)
【分类号】：R749.4

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