不同亚型阿尔茨海默病患者血脂、胰岛素代谢和对氧磷酶-2基因311多态性

发布时间：2018-02-25 22:21

本文关键词： 阿尔茨海默病对氧磷酶-基因基因多态性胰岛素血脂　出处：《中国老年学杂志》2014年19期 　论文类型：期刊论文

【摘要】：目的探讨根据中国精神障碍分类与诊断标准第3版(CCMD-3)诊断的老年型、老年前期型和混合型阿尔茨海默病(AD)患者间血脂、胰岛素代谢、对氧磷酶-2(PON2)311基因多态性的差异和特点。方法研究对象包括84例AD患者,符合美国国立神经病学、语言障碍和卒中研究所-AD和相关疾病协会(NINCDS-ADRDA)"可能AD"的诊断标准,无痴呆家族史。根据CCMD-3分为老年型、老年前期型和混合型三组,用聚合酶链反应-限制性片段长度多态性分析法检测患者PON2基因311位点多态性,检测血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、空腹血糖(FBP)。利用化学发光法测空腹血清胰岛素。利用基础状态法评定胰岛素抵抗性,临床上可使用胰岛素敏感指数(ISI)表达。应用简明智能评定量表(MMSE)、日常生活能力量表(ADL)、Hachinski缺血量表等评定三组患者的认知等临床特征。分析三组间PON2 C311S基因多态性、血清胰岛素、血糖、血脂和神经心理学量表得分的差异。结果老年前期型AD组的血清TG和TC高于老年型和混合型AD组(P0.01)。老年型AD组的HDL水平高于混合型AD组(P0.01)。老年前期型AD组的血清胰岛素水平高于老年型和混合型AD组(P0.01)。老年型、老年前期型和混合型AD三组间的PON2 311基因型C/C、C/S和S/S分布差异无统计学意义,3组间C和S等位基因频率也无统计学差异。结论老年前期型AD患者应加强血脂和胰岛素代谢的重视和干预。不同亚型的AD患者PON2基因311位点基因型及等位基因频率未发现显著差异。
[Abstract]:Objective to investigate the serum lipids and insulin metabolism in patients with senile, presenile and mixed Alzheimer's disease (AD) diagnosed by CCMD-3 according to the Chinese Classification and Diagnostic criteria for Mental Disorders (CCMD-3). Methods Eighty-four patients with AD were included in the study, which met the diagnostic criteria of NINCDS-ADRDA-NINCDS-ADRDA-NINCDS-ADRDA.Methods 84 patients with AD were included in the study, which met the diagnostic criteria of National Institute of Neurology, speech Disorder and Stroke (NINCDS-ADRDAA). There was no family history of dementia. CCMD-3 was divided into three groups: senile type, presenile type and mixed type. Polymerase chain reaction-restriction fragment length polymorphism (PCR) was used to detect the polymorphism of PON2 gene. Serum cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), fasting blood glucose (FBPN), fasting serum insulin (fasting insulin) were measured by chemiluminescence, and insulin resistance was assessed by basic state method. Insulin sensitivity index (ISI) expression was used in clinic. The cognitive characteristics of the three groups were evaluated with the brief Intelligent Assessment scale (MMSE) and the activity of Daily living (ADL) / Hachinski Ischemia scale. The polymorphism of PON2 C311S gene and serum insulin were analyzed among the three groups. Blood sugar, Results the levels of serum TG and TC in AD group were higher than those in AD group of senile type and mixed type AD group (P 0.01). The level of HDL in senile type AD group was higher than that in mixed AD group (P 0.01). The serum level in pre-senile AD group was higher than that in senile AD group. The insulin level was higher than that in the aged and mixed AD groups (P 0.01). There was no significant difference in the distribution of C / C / C / S and S / S alleles between the three groups. Conclusion the serum lipids and pancreatic islets should be strengthened in AD patients with pregeriatric type AD, and there is no significant difference in the frequency of C and S alleles among the three groups. There was no significant difference in genotype and allele frequency of PON2 gene 311 locus in AD patients with different subtypes of AD.
【作者单位】：广州市脑科医院老年精神科;
【基金】：广东省自然科学基金资助项目(No.8151037001000006)
【分类号】：R749.16

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