青春期亚慢性地卓西平暴露对小鼠成年期前脉冲抑制和糖耐量的影响

发布时间：2018-02-28 01:27

本文关键词： 地卓西平精神分裂症前脉冲抑制糖耐量　出处：《上海交通大学学报(医学版)》2014年06期 　论文类型：期刊论文

【摘要】：目的探讨青春期亚慢性地卓西平(MK-801)注射对小鼠成年期前脉冲抑制(PPI)和糖耐量的影响。方法 20只青春期小鼠随机分为对照组(n=10)和模型组(n=10),两组小鼠出生后30~43 d分别接受0.9%生理盐水(4 mL/kg)和MK-801(0.6 mg/kg)注射,1次/d,持续2周。每周记录摄食和体质量,并于小鼠成年期(出生后70~80 d之间)进行旷场试验、PPI测试和葡萄糖耐量试验(GTT)。结果模型组小鼠在79 dB强度的前脉冲刺激时,PPI显著低于对照组(P0.05),表明MK-801成功诱导小鼠精神分裂样行为。模型组小鼠体质量也显著低于对照组(P0.05)。GTT结果显示:模型组小鼠注射葡萄糖后90 min和120 min的血糖水平显著高于对照组(P0.001和P0.01),表明MK-801诱导的精神分裂样小鼠模型出现糖耐量异常。两组小鼠的摄食量和旷场试验中的自发活动量差异均无统计学意义(P0.05)。结论青春期亚慢性注射MK-801可诱导小鼠成年期PPI损害和糖耐量异常。
[Abstract]:Objective to investigate the effects of subchronic Dizoxipine MK-801) injection on preadulthood pulse inhibition of PPI) and glucose tolerance in mice. Methods Twenty puberty mice were randomly divided into two groups: control group (n = 10) and model group (n = 30, 43 d after birth). Don't take 0.9% saline (4 mL / kg) and MK-801(0.6 mg / kg once a day for 2 weeks. Record your food intake and body mass weekly. Open field test (PPI) and glucose tolerance test (GTT) were performed in adult mice (between 70 and 80 days after birth). Results the PPI of the model group was significantly lower than that of the control group at 79 dB intensity prepulse stimulation, indicating that MK-801 was successfully induced small. The body mass of mice in the model group was significantly lower than that in the control group (P 0.05). The results showed that the blood glucose levels in the model group at 90 min and 120 min after glucose injection were significantly higher than those in the control group (P 0.001 and P 0.01), indicating that the psychosis induced by MK-801 in the model group was significantly higher than that in the control group (P 0.001 and P 0.01). There was no significant difference between the two groups in food intake and spontaneous activity in open field test (P 0.05). Conclusion Subchronic injection of MK-801 in puberty can induce PPI damage and impaired glucose tolerance in adult mice.
【作者单位】：上海交通大学医学院附属精神卫生中心上海市重性精神病重点实验室;美国耶鲁大学医学院内科;
【基金】：国家自然科学基金面上项目(81171266,81271481)~~
【分类号】：R749.3

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